Aspects of the biology and preliminary assessment of DNA quality and quantity of two cichlids from Lagos Lagoon, Nigeria

Olufemi O. Soyinka, Mutiu O. Sifau, Peace I. Adamu

Abstract


Aspects of the growth pattern, reproductive biology, and molecular characteristics of two cichlids, Sarotherodon melanotheron and Coptodon zillii were investigated in Lagos Lagoon. A total of twenty-nine individual species were collected from the Lagos Lagoon. Blood samples were obtained for DNA extraction and analysis from 9 specimens of each species. The morphometric data of S. melanotheron varied from 2.5-4.5cm (head length), 13.5-18.0cm (total length) and 35.00-135.00g (body weight), while that of C. zillii were 2.0-4.5cm, 12.0-21.5cm and 39.00-228.00g respectively. The growth pattern of S. melanotheron showed a positive allometric, b= 3.324, while that of C. zillii showed a negative allometric pattern with b= 2.715. The condition factors of S. melanotheron and C. zillii ranged from 4.15-4.55 and 4.12-5.07 respectively. The sex ratio for S. melanotheron was 1: 0.18, while C. zillii had a sex ratio of 1:0.33, and these were statistically significant (p< 0.05). From the molecular characterization analysis, the DNA concentration ranged from 48-1265ng/µl and DNA purity ranged from 1.49-1.82. These findings form baseline information for conducting further investigations on the taxonomy, ecology, conservation of the genetic purity of these fisheries resources in Lagos Lagoon.


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Aboaba M. A. 1993. Reproduction, larval rearing, and the influence of dietary protein on the growth of the catfish (Chrysichthys nigrodigitatus).Ph.D. Thesis, University of Ibadan, Ibadan, Nigeria.5-25p.

Adeyemi S.O., Bankole, N.O., Adikwu, I.A., Akombo, P.M. 2009. Age, growth and mortality of some commercially important fish species in Gbadikere Lake, Kogi state, Nigeria. Int. J. Lakes Rivers Res. India Pub., 2 (1): 63-69

Ahmed MMM, Ali BA, El-Zaeem, SY. 2004. Application of RAPD markers in fish: Part I- Some genera (Tilapia, Sarotherodon, and Oreochromis) and species (Oreochromis aureus and Oreochromis niloticus) of Tilapia. Int. J. Biotechnol. 6:86-93.

Ali, B.A., Huang, T.H., QinDa N., Wang, X.M. 2004. A review of random amplified polymorphic DNA (RAPD) markers in fish research. Review in Fish Biology and Fisheries 14:443-453

Anene, A. 2005. Condition factor of four cichlid species of a man-made in Imo state, South Eastern Nigeria. Turk. J. Aq. Sc. 5: 43 - 47.

Arawomo, G.A.O. 1982. The growth of Sarotherodon niloticus (L) in Opa reservoir, Proceedings of the 2nd Annual Conference of the Fisheries Society of Nigeria, University of Ife, Ile-Ife, Nigeria pp. 221 - 227.

Avtalion R. R., Duczyminer M., Wodjani A., Pruginin, Y. 1976. Determination of allogeneic and xenogenetic markers in the genus of Tilapia: II. Identification of T. aurea, T. vulcani, and T. nilotica by electrophoretic analysis of their serum proteins. Aquaculture 7:255-265.

Ayoola, S.O., Kuton, M.P. 2009. Seasonal variation in fish abundance and physicochemical parameters of Lagos lagoon, Nigeria. African Journal of Environmental Science and Technology, 3 (5): 149-158.

Bo-young Woo-Jai L., Todd, S. J., Karen, L.C, Aimee E.H., Gideon, H., Audun, S., Justin E.S., Terai Y, Kocher, T.D. 2005. A second-generation genetic linkage map of tilapia (Oreochromis niloticus) Genet. 17:237-244.

Braga, F. M., Gennari, F. 1990. Contribution for the knowledge of the reproduction of Moenkhausia mediates (Characidae, Tetragonopterinae), in the dam of Pretty Bar. Sao Paulo, Brazil. 15: 171-188.

Crosetti, D., Sola, L., Brunner, P, Cataudella, S. 1988. Cytogenetical characterization of Oreochromis niloticus, O. mossambicus, and their hybrid. In: R.S.V. Pullin, T. Bhukaswan, K. Tonguthai and J. L. Maclean (eds.) the Second International Symposium on Tilapia in Aquaculture. ICLARM Conference Proceedings 15. Department of Fisheries, Bangkok, Thailand and International Center for Living Aquatic Resources Management, Manila, Philippines. pp. 143-151.

Emmanuel, B.E., Chukwu, L.O., Azeez, L.O. 2008. Gillnet selectivity and catch rates of pelagic fish in tropical coastal lagoon ecosystem. African Journal of Biotechnology 7(21): 3962-760.

Fagade, S.O. 1982. The food and feeding habits of Sarotherodon galilaeus from a small lake. Archiv. Für Hydro. 97: 60 - 73.

Fagade, S.O. 1983a. The breeding cycle of Sarotherodon galilaeus the IITA Lake, Ibadan, Nigeria. Archiv, für.Hydro.100: 493 - 55.

Fagade, S.O. 1983b. The biology of Chromidotilapia guntheri from a small lake. Arch. Hydrobiol. 97: 60 - 72.

Fagade S.O., Olaniyan C.I.O. 1974. Seasonal distribution of the fish fauna of the Lagos Lagoon. Bull. De IFAN Ser. A, 36(1): 244-254.

Famoofo, O.O., Abdul, W.O. 2020. Biometry, condition factors and length-weight relationships of sixteen fish species in Iwopin fresh-water ecotype of Lekki Lagoon, Ogun State, Southwest Nigeria, Heliyon, 6 (1)

Faunce, C.H., Patterson, H.M., Lorenz, J.J. 2002. Age, growth, and mortality of the Mayan cichlid (Cichlasomau rophthalmus) from the South-Eastern Everglades. Fish. Bull. 100: 42 - 50.

Froese, R. 2006. Cube law, condition factor, and weight–length relationships: history, meta-analysis, and recommendations. J. Appl. Ichthyol. 22, 241–253. doi:10.1111/j.1439-0426.2006.00805.x

Froese, R., Pauly, D. 2007. "Cichlidae" in Fish Base... Nov 2007 version.www.fishbase.org.

Harris, A.S., Bieger, S., Doyle, R.W., Wright, J.M. 1991. DNA fingerprinting of tilapia, Oreochromis niloticus, and its applications to aquaculture genetics. Aquaculture 92:157-163.

Hill, M.B., Webb, J.E. 1958. The Ecology of Lagos Lagoon II.Topography physical features of Lagos Harbour and Lagos Lagoon. Phil Trans. B. 241: 391 -333.

Ikomi, R.B., Jessa, H.O. 2003. Studies on aspects of the biology of Tilapia mariae (Boulenger, 1899) (Osteichthyes Cichlidae) in Ethiope River, Niger Delta, Nigeria. Afri.Zoo. 38(2): 255 - 264.

Khallaf, E., Galal, M., Athuman, M. 2003. The biology of Oreochromis niloticus in a polluted canal. Ecotoxicology, 12: 405-416.

Koetsier, G., Cantor, E. 2019. A practical guide to analyzing nucleic acid concentration and purity with micro-volume spectrophotometers. Technical note. New England Biolabs, 8pp.

.Kullander, S. O. 1998. A phylogeny and classification of the South American Cichlidae (Teleostei: Perciformes). In Phylogeny and classification, of Neotropical fishes Edited by: Malabarba LR, Reis RE, Vari RP, Lucena ZM, Lucena CAS. Porto Alegre: EDIPUCRS; 1998:461-498.

Kuton, M. P., Kusemiju, K. 2010. Species diversity and richness of cichlids in three Southwest lagoons in Ghana. Journal of Scientific Research and Development, 12, 22 – 33.

Lee, W.J., Kocher, T.D. 1996. Microsatellite DNA markers for genetic mapping in Oreochromis niloticus. J. Fish Biol. 49:169-171.

Lee, W.J., Kocher, T.D. 1998. Microsatellite mapping of the prolactin locus in the tilapia genome. Anim. Genet. 29:68.Macaranas MJ, Augstin LQ, Ablan MCA, Pante MJR, Knath AAE, Paulin RSV (1986). Genetic improvement of farmed tilapias: biochemical characterization of strain differences in Nile Tilapia. Aquaculture international 3:43-54.

LI Si-Fa, ZHAO Yan, FAN Wu-Jiang, CAI Wan-Qi, XU Ying-Fang. 2011. Possible genetic reproductive isolation between two tilapia genera and species: Oreochromis niloticus and Sarotherodon melanotheron. Zoological Research, 32(5): 521−527

Marcus, O. 1982. The Biology of the Clupeid, Ilisha africana (Bloch) off the Nigerian Coast. Ph.D. Thesis, University of Lagos, Nigeria. 209 pp

McConnel, S.K.J., Beynon, C., Leamon, J., Skibinski, D.O.F. 2000. Microsatellite marker-based genetic linkage maps of Oreochromis aureus and O. niloticus (Cichlidae): extensive linkage group segment homologies revealed. Anim. Genet. 31:214-218.

Meye, J.A. 2012. Length-weight Relationships and Condition Factor of Tilapia zillii (Gervais, 1848) from River Orogodo, Delta State, Nigeria, Journal of Aquatic Sciences, 27(2)

Olaniyan, C. I. O. 1957. The seasonal variation in plankton variation in Lagos Harbour, Nigeria. Ph.D. Thesis, London University. 173 pp.

Olufeagba, S.O., Aladele, S.E., Okomoda, V.T., Sifau, M.O., Ajayi, D.A., Oduoye, O.T., Bolatito, O.A., Nden, D.S., Fabunmi-tolase, A.S., Hassan, T. 2015. Morphological Variation of Cichlids from Kainji Lake, Nigeria. Journal of FisheriesSciences.com, 9(3): 070-080

Omoniyi, I.T. Agbon, A.O. 2008. Morphometric variations in Sarotherodon melanotheron (Pisces: Cichlidae) from Brackish and Freshwater habitats in South-western Nigeria. West African Journal of Applied Ecology, 12, 1 – 5.

Pante, M.J.R., Lester, L.J., Pullin, R.S.V. 1988. A preliminary analysis on the use of canonical discriminant analysis of morphometric and meristic characters to identify cultured tilapias. In: The Second International Symposium on Tilapia in Aquaculture (Pullin R.S.V., Bhukaswan T., Tonguthai K. and MacClean J.L. (Eds.)). ICLARM Conference Proceedings 15, Manila, Philippines. pp. 251-257.

Poletto A.B., Ferreira I.A., Cabral-de-Mello D.C., Nakajima R.T., Mazzuchelli J., Ribeiro H.B., Venere P.C., Nirchio M., Kocher, T.D., Martins C. 2010. Chromosome differentiation patterns during cichlid fish evolution. BMC Genet. 2010 Jun 15; 11:50. DOI: 10.1186/1471-2156-11-50. Erratum in: BMC Genet. 2012 Jan 19; 13:2. PMID: 20550671; PMCID: PMC2896337.

Prasad, G. 2001. Length-weight relationship of P. monodon reared in semi-intensive culture system of Kerala, India. Naga ICLARM Quart., 24 (1&2): 18-19.

Seyoum, S. 1989. Stock identification and the evolutionary relationship of the genera Oreochromis, Sarotherodon, and Tilapia (Pisces: Cichlidae) using allozyme and analysis and restriction endonuclease analysis of mitochondrial DNA, Ph.D. thesis, University of Waterloo, Waterloo, Ontario, Canada.

Seyoum, S., Kornfield, I. 1992. Taxonomic notes on the Oreochromis niloticus subspecies complex (Pisces: Cichlidae), with a description of a new subspecies. Can. J. Zool. 70:2161-2165.

Soyinka, O.O. 2010. The Bio-ecology and culture potentials of the grey mullet, Mugil cephalus (Linnaeus) from the Lagos Lagoon, Nigeria. Ph.D. Thesis, University of Lagos, 223pp.

Soyinka, O.O. 2011. Growth pattern and condition factor in relation to the salinity of the grey mullet, Mugil cephalus (Linnaeus) from Lagos Lagoon. Nigeria Journal of Fisheries, 8 (2): 339 – 345

Soyinka, O. O. 2017. Reproductive studies of striped mullet (Mugil cephalus) from the high brackish tropical lagoon. Livestock Research for Rural Development, 29(8)

Soyinka, O.O., Kassem, A.O. 2008. Seasonal distribution and fish species diversity of a tropical lagoon in the south-west, Nigeria. Journal of Fisheries and Aquatic Sciences 3(6): 375-383.

Soyinka, O.O., Adewale, T.A., Banjoko, B.L., Okereke, B.O. 2020. Observations on the fish species composition of Lagos Lagoon after four decades of investigation. Journal of Scientific Research and Development, 19(2)

Soyinka O.O., Ojo, O.O. 2015. Growth pattern, condition factor and heavy metal concentration in black chin tilapia, Sarotherodon melanotheron from Lagos Lagoon. Journal of Aquatic Sciences. 30(1B): 119 – 128.

Swain, D.P., Foote, C.J. 1999. Stocks and chameleons the use of phenotypic variation in stock identification. Fisheries Research.43, 113-128

Thomas, J., Venus, S.D., Kurup, B.M. 2003. Length-weight relationship of some deep sea fishes inhabiting the continental slope beyond 250 m depth long west coast of India. Naga LCLARM Quart. 26, 17-21.

Trewavas, E. 1983. Tilapiine fishes of the Genera Sarotherodon, Orechromis, and Danakilia. Brit Muse. Naturl. Hist., 583p.

Turin. C., Erguden, D., Turin, F., Gurlek, M. 2004. Genetic and morphologic structure of Liza abu (Heckel, 1843) populations from the Rivers Orontes, Euphrates, and Tigris. Turkish Journal of Veterinary and Animal Science. 28: 729-734.

Wilfinger, W.W., Mackey, K., Chomczynski, P. 1997. Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity: BioTechniques 22:474-481.

Ugwumba, A. A. A. 1988. Food and feeding habits of the juveniles of some culturable fish species in Nigeria. Nigerian Institute for Oceanography and Marine Research Technical Paper No. 31. 24 pp.

Ugwumba, A. A. A. 1990. Food and feeding ecology of Oreochromis niloticus, Sarotherodon melanotheron, and Heterotis niloticus (Pisces: Osteichthyes) in Awba reservoir Ibadan. Ph.D. Thesis, University of Ibadan, Ibadan, Nigeria, 395 pp.




DOI: https://doi.org/10.13170/ajas.7.1.22913

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