Detection Of Antibiotic Residues in Eggs of Layer Chickens and Knowledge of Animal Officers About Antibiotics

Jefrianda Jefrianda, Nurliana Nurliana, Darmawi Darmawi, Teuku Reza Ferasyi, Sugito Sugito


The chicken farming industry in Indonesia cannot be separated from the use of antibiotics as a curative effort in controlling the disease. Its use is either via injection, soaking, or as a feed additive (growth promoter) mixed in feed. Inappropriate use of antibiotics causes allergic reactions or resistance and the possibility of causing poisoning, residues in livestock tissues or organs that are harmful to human health when consuming them. This study aimed to determine the antibiotic residues in eggs and the level of knowledge of livestock workers on the use of antibiotics in livestock. The detection of antibiotic residues was conducted using the agar plate diffusion method by Kirby Bauer with the disk diffusion technique using Escherichia coli and Salmonella sp. As indicator bacteria. The results were analysed using a qualitative descriptive approach.

The samples were 76 eggs taken from the Regional Technical Implementation Unit of the Non-Ruminant Livestock Center of the Aceh Livestock Service in Blang Bintang and Saree Aceh Besar. Chloramphenicol antibiotic was used as a test medium and as control by calculating the diameter of the inhibition zone formed on MHA media. furthermore, a cross-sectional study design was used and questionnaires were distributed to 14 livestock officers to understand the knowledge of officers on the use of antibiotics in livestock by using google form. The data were analyzed using the chi-square test through the SPSS 22.0 program.

The results showed that the eggs and feed samples were negative from antibiotic residue. There was no relationship between feeding and antibiotic residues in eggs and laying hens feed. However, only 35.7% of officers had good knowledge about proper antibiotic usage. This means continuing awareness to the officers regarding the use of antibiotics either for treatment of diseases or as feed promoter are essential. 


Laying hens; antibiotic residue; antibiotic resistance

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Ariyani, N., Nurhidayah, Istianingsih, Ambarwati, and R.A. Sari. 2018. Doxycycline and ciprofloxacin resistance in Escherichia coli Isolated from Layer feces. Proceeding: Musyawarah Nasional ke III Asosiasi Farmakologi dan Farmasi Veteriner Indonesia = 3rd National Conference of Indonesia Veterinary Pharmacy and Pharmacology Association. 6–11.

Asredie, T. and Engdaw, T.A. 2015. Antimicrobial Residues in Cow Milk and its Public Health Significance, World Journal of Dairy & Food Sciences, 10(2), p.147–153. Central Bureau of Statistics (BPS). 2019. Livestock and Animal Health Statistics: Livestock and Animal Health Statistics 2019. Jakarta: Directorate General of PKH, Ministry of Agriculture of the Republic of Indonesia. Jakarta.

Davis, J.L, Foster, D.M and Papich, M.G 2007. Pharmacokinetics and tissue distribution of enrofloxacin and its active metabolite ciprofloxacin in calves. J Vet Pharmacol Therap, 30, p. 564–571.

Dewi, A.A.S., N.P. Widdhiasmoro, I. Nurlatifah, N. Riti, and D. Purnawati. 2014. Residues of Antibiotics in Foods of Animal Origin, Impacts and Management Efforts. Veterinary Bulletin, BBVet Denpasar. 26(85).

Donkor, E.S., M.J. Newman, S.C.K. Tay, N.T.K.D. Dayie, E. Bannerman, M. Olu-Taiwo. 2011. Investigation into the risk of exposure to antibiotic residues contaminating meat and egg in Ghana. Food Control. 22(6): 869-873.

Etikaningrum, E. and Iwantoro S. 2017. Study of Antibiotic Residues in Poultry Livestock Products in Indonesia. Journal of Animal Production Science and Technology. 5(1): 29-33.

Insany A.N., D.P. Destiani, A. Sani, L. Sabdaningtyas, I.S. Pradipta. 2015. Association between Perceived Value and Self-Medication with Antibiotics: An Observational Study Based on Health Belief Model Theory. Indonesian Journal of Clinical Pharmacy. 4(2): 77–86.

Ministry of Agriculture. 2019. The current situation and government policies regarding Antimicrobial Resistance (AMR) in the Livestock and Animal Health Sector,” said the AMR Studio Generale at Menara 165 Jakarta.

Kusumawardani, A. 2016. Description of the sensitivity of various antibiotics and the profile of Escherichia coli plasmid isolates from dug well water in Demak Regency. Thesis. Muhamadiyah Semarang University, Semarang.

Meutia, N. T. Rizalsyah, S. Ridha, and M. K. Sari1. 2016. Antibiotic Residuesin Water Fresh Milk Derived from Farms in The Territory of Aceh Besar. Journal Animal Science Padjadjaran University. 16(1): 1-5.

Nghiem, M.N., V.T. Nguyen, T.T.H. Nguyen, T.D. Nguyen, T.T.B. Vo. 2017. Antimicrobial resistance gene expression associated with multidrug resistant Salmonella spp. isolated from retail meat in Hanoi, Vietnam. International Microbiology. 20(2): 85-93.

Noviana, H. 2004 “Patterns of Antibiotic Sensitivity to Escherichia coli Isolated from Various Clinical Specimens. Trisakti Medical Journal. 23:1-5.

Nurhasnawati, H., S. Jubaidah, and N. Elfia. 2016. Freshwater Circulating in Segiri Market Using Ultra Spectrophotometric Method. Manuntung Scientific Journal, 2(2): 173–178.

Palupi, M.F., M.I.N. Rahminiwati, and U. Patriana. 2009. Pharmacokinetics of paracetamol in chicken plasma (Gallus domesticus). BBPMSOH. 16: 10-14.

Priyanka, S. Panigrahi, M.S. Sheoran, and S. Ganguly. 2017. Antibiotic residues in milk-a serious public health hazard. Journal of Environment and Life Sciences J Environ Life Sci. 2(4): 99-102.

Purnawarman, T. and Efendi, R. 2020. Knowledge, Attitudes, and Practices of Breeders in Using Antibiotics in Broiler Chickens in Subang Regency. Acta VETERINARIA Indonesiana. (3): 48–55.

Rahayu I. 2014. Principles of husbandry corner treatment. Available at: http://im [Accessed April 8, 2021].

Sholih, MG, Muhtadi, A. and Saidah, S. 2015. Rationality of Antibiotic Use in a General Hospital in Bandung in 2010. Indonesian Journal of Clinical Pharmacy. 4(1): 63–70.

Indonesian National Standard (SNI). 2008. SNI 3926: 2008 Chicken Eggs Consumption, Indonesian National Standard. 1–8.

Suharsono. 2010. Probiotics Scientific Base, Application and Practical Aspects. Bandung: Publisher Widya Padjajaran.

Sumambang, A., A.M. Ariyanto, A. Kompudu, D.M. Pangaribuan, E. Nugroho, R.M. Puspita, and D. Ulfa. 2019. Farmers' Perceptions of Antibiotic Use in Commercial Broiler Farms in West Kalimantan Province. Proceedings of the Investigation of Animal Diseases Technical Meeting and Scientific Meeting (RATEKPIL) and Animal Health Surveillance 2019. 482–488.

Thongpalad, K., J.K.M. Kuwornu, A. Datta, S. Chulakasian, and A.K. Anal. 2019. On-farm food safety knowledge, attitudes and self-reported practices of layer hen farmers. Br Food J. 121(8): 1912–192.

Walyani, S., T. Purnawarman, and E. Sudarnika. 2019. Prevalence of Salmonella Spp. Bacteria Antibiotic Resistency Indigestion Tract in the Broiler Farms of Subang District. Bulletin of Animal Science. 43(1): 474-481.

Wijaya, M.R. 2011. Residues of Antibiotics in Chicken and Beef from Traditional Markets in West Java Province, Faculty of Veterinary Medicine. Thesis. Bogor Agricultural University.

Yulianti NT et al. 2016. Study of antibiotic residues of the tetracycline and penicillin groups in the meat, liver, intestines and lungs of broiler chickens in 13 provinces in Indonesia. Gunung Sindur Center for Quality Testing and Certification of Veterinary Drugs. 25: 20–25.

Yuningsih. 2005. Presence of Antibiotic Residues in Livestock Products. 48–55.


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