The accuracy of progesterone test kits for early diagnosis in Ettawa cross-goat
Abstract
This research aimed to determine the accuracy of the progesterone test kit for milk and blood for early pregnancy diagnosis in Ettawa cross goats (PE). Five lactating PE goats were used in this study and were synchronized with PGF2α intramuscularly. The result is that all goats showed a sign of estrus. Four goats were mated naturally, while one other was not mated. The determination of day 0 was when the females were standing heat. The early pregnancy diagnosis using dairy cow test strips was performed 18-22 days after mating or post-estrus. The accuracy of the diagnosis was confirmed by ultrasound on day 35 after mating/post estrus. Diagnosis using the milk progesterone kit showed negative results for five days of examination in all goats, whereas using the blood progesterone kit showed positive results in four goats. When confirmed, the ultrasound showed that one goat was not pregnant, and four other goats were pregnant. It can be concluded that the pregnancy test strip kits containing progesterone are effective in diagnosing early pregnancy in PE goats with high accuracy in blood samples (100%) despite low accuracy in milk samples (20%).
Keywords
Full Text:
PDFReferences
Amer, H.A. (2008). Determination of first pregnancy and fetal measurements in Egyptian baladi goats (Capra hircus). Vet. Italiana, 44(2), 429-437.
Boscos, C. M., Samartzi, F. C., Lymberopoulos, A. G., Stefanakis, A., & Belibasaki, S. (2003). Assessment of progesterone concentration using enzyme immunoassay for early pregnancy diagnosis in sheep and goats. Reproduction in domestic animals = Zuchthygiene, 38, 170-174. https://doi.or g/10.1046/j.1439-0531.2003.00407.x.
Bretzlaff, K. N., Elmore, R. G., & Nutti, L. C. (1989). Use of an enzyme immunoassay to determine concentrations of progesterone in caprine plasma and milk. Journal of the American Veterinary Medical Association, 195(4), 664-668.
Broaddus, B. & De Vries, A. (2005). A comparison of methods for early pregnancy diagnosis. Proceedings. 2nd Florida Dairy Road Show, Florida.
Dionysius, D. A. (1991). Pregnancy diagnosis in dairy goats and cows using progesterone assay kits. Australian Veterinary Journal, 68(1),14-16. https:// doi.org/10.1111/j.1751-0813.1991.tb098 32.x.
Engeland, I. V., Ropstad, E., Andresen, O., & Eik, L. O. (1997). Pregnancy diagnosis in dairy goats using progesterone assay. Animal reproduction science, 47(3), 237–243. https://doi.org/10.1016/s0378-4320( 96)01632-6.
Fleming, S. A., Camp, S. D. V., & Chapin, H. M. (1990). Serum progesterone determination as an aid for pregnancy diagnosis in goats bred out of season. The Canadian veterinary journal = La revue veterinaire canadienne, 31(2), 104-107.
Hafez, E. S. E. (2000). Sheep and Goats in Hafez. Reproduction in Farm Animals. 7th ed. Lea and Febiger, Philadelphia, USA.
Heap, R. B., Gwyn, M., Laxing, J. A., & Waltrex, D. E. (1973). Pregnancy diagnosis in cows; changes in milk progesterone concentration measured during estrous cyclic and pregnancy by radio immune assay. J. Agricultural Research, 81,151.
Holdsworth, R.J. & Davis, J. (1979). Measurement of progesterone in goats' milk: an early pregnancy test. The Veterinary Record, 105(23), 535.
Islam, M.M., Kizaki, K., Takahashi, T., Khanom, J. S., Debnath, S., & Khandoker, M. (2014). Pregnancy diagnosis in black Bengal goat by progesterone assay. Bangladesh Journal of Animal Science, 43(3), 180-184. https://doi.org/10.3329/bjas.v43i3.21645.
Karadaev, M. (2015). Pregnancy diagnosis techniques in goats- a review. Bulgarian Journal of Veterinary Medicine, 18(3), 1–11. https://doi.org/10. 15547/bjvm.837.
Karen, A., Szabadoz, K., Reiczigwl, J., Beckers, J.F., & Szenci, O. (2004). Accuracy of transrectal ultrasonography for determination of pregnancy in sheep: effect of fasting and handling of the animals. Theriogenology, 61(7-8), 1291-1298. https://doi.org/10.1016/j.theriogeno logy.2003.07.018.
Lestari, D. L. (2006). Metode Deteksi Kebuntingan Pada Ternak Sapi. Fakultas Peternakan, Universitas padjadjaran, Bandung.
Maryati, T. & Nuniek, L. (1990). Penentuan kandungan hormon progesteron dalam darah dan susu pada kambing dan sapi. Risalah Pertemuan Ilmiah Pusat Aplikasi Radio Isotop. 645-658.
Melia, J, Fauziah, Hamdan, Panjaitan, B., Syafruddin, Armansyah, T. R, & Siregar, T. N. (2018). The image of the embryo and fetal of Ottawa crossbred goats using ultrasonography at different gestational ages. Jurnal Kedokteran Hewan, 12(2), 47-52. https://doi.org/10.21157/j.ked.hew an.v12i2.3768.
Nebel, R. L. (1998). On Farm progesterone tests. J. Dairy Science, p. 71, 1682–1690. https://doi.org/10.3168/jds.S0022-0302(88)79733-7
Nova, M. E., Ryadi, G., & Melia, J. (2014). Diagnosis kebuntingan dini menggunakan kit progesteron air susu pada kambing peranakan etawah (Capra hircus). Jurnal Medika Veterinaria, 8(2): 120-124. https://doi.org/10.21157/j.med.v et..v8i2.3350.
O’Connor, M .L. (2003). Milk Progesterone Analysis for Determining Reproductive Status. College of Agricultural Sciences, Cooperative Extension Compendium, The Pennsylvania State University.
Siregar, T. N. dan Hamdan. (2008). Teknologi Reproduksi pada Ternak. Fakultas Kedokteran Hewan, Universitas Syiah Kuala, Banda Aceh.
Syafruddin, Rusli, Hamdan, Rozlizawaty, Rianto, S., & Hudaya, S. (2012). akurasi metode observasi tidak kembali berahi (Non-Return to Estrous) dan ultrasonography (USG) untuk diagnosis kebuntingan peranakan etawah. Jurnal Kedokteran Hewan , 6(2), 89-91. https: //doi.org/10.21157/j.ked.hewan.v6i2.308.
Wani, N. A., Wani, G. M., Mufti, A. M., & M.Z. Khan. (1998). Ultrasonic pregnancy diagnosis in gaddi goats. Small Ruminant Research, 29,239-240. https://d oi.org/10.1016/S0921-4488(97)00123-5.
DOI: https://doi.org/10.21157/ijtvbr.v8i1.32045
Article Metrics
Abstract view : 0 timesPDF - 0 times
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Indexed in:
Copyright© 2016 | ISSN: 2503-4715
Published by:
The Faculty of Veterinary Medicine of Syiah Kuala University
In cooperation with:
Center for Tropical Veterinary Studies of Syiah Kuala University
and Indonesian Veterinary Medical Association (PDHI)
Online Submissions & Guidelines | Editorial Policies | Contact | Statistics
IJTVBR is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.