Screening of Pathogenic and Non-Pathogenic Bacteria Isolated from Maggot Digestive System
Abstract
Maggots are one of the fly larvae with a high protein content of up to 30-45%, so maggots can be used as an alternative animal feed ingredient. As one of the sources of animal feed raw materials, insects-based feed must also be safe from contaminants of microorganisms, including pathogenic bacteria. Therefore, this study aims to isolate and determine the screening of pathogenic and non-pathogenic bacteria from maggots' digestive systems. Maggots are cultivated on media based on animal faecal and organic waste for ± 2 months. Then, the bacteria are isolated and purified using nutrient agar media, and hemolysis tests are carried out on blood agar media and Gram staining. Eighty-seven bacterial colonies with various morphological characteristics were successfully isolated from the maggot digestive system. Most isolated bacteria are classified as Gram-negative bacteria with a bacilli form. Based on the results of the hemolysis test, as many as 16% of bacterial isolates are indicated by pathogenic bacteria because of their ability to hemolyze blood. However, only about 2% showed b-hemolysis. Thus, it can be concluded that the screening results of non-pathogenic bacteria are still more numerous compared to pathogenic bacteria present in the maggot digestive system.
Keywords
Full Text:
PDFReferences
Afkar, K., Masrufah, A., Fawaid, AS, Alvarizi, DW, Khoiriyah, L.,Khoiriyah, M., Kafi, MA,
Faradilla, RS, Amsah, R., Hidayah, NN, Salsabella, A., Nazwa, DAR, Fadila, SN, Sari, UEK, Naim, FI, Itsnaini, SNR and Ramadhan, MN. (2020). BSF maggot cultivation (Black Soldier Fly) as an alternative feed for catfish (Clarias batracus) in Candipari Village, Sidoarjo on a holistic development and empowerment program for dea (PHP2D). J. Sci. Soc. Develop. 3,10-16.
Amandanisa, A. and Suryadarma, P. (2020). Study of nutrition and cultivation of maggot
(Hermentia illuciens L.) as an alternative fish feed in RT 02 Purwasari Village, Dramaga District, Bogor Regency. J. Comm. Innov. Center, 2(5),796‒804.
Zhang, S., Dennis, GABO and Wouter, HH (2014). Protein quality of insects as potential ingredients for dog and cat foods. J. Nutr. Sci., 3, 1-4.
Erickson, MC, Islam, M., Sheppard, C., Liao, J. and Doyle, MP. (2004). Reduction of Escherichia coli O157:H7 and Salmonella enterica serovar Enteritidisin chicken manure by larvae of the Black Soldier Fly. J. Food Prot. 67, 685-690.
Fahmi, MR, Hem, S. and Subamia, IW. (2009). Maggot potential for upgrade growth and
health status of fish. J. Ris. Aquac 4(2), 221-232.
Fahmi, MR. (2010). Management of maggot development towards the independent mina feed area. In: Proceedings of the Aquaculture Technology Innovation Forum. Jakarta (Indonesia): Center for Fisheries Research and Development. p. 763-767.
Fatmasari, L. (2017). The level of population density, weight, and maggot length (Hermetia
illucens) on different media.Thesis. Raden Intan State Islamic University, Lampung. Gulaydin,
O., Ekin, IH, Ozturk, C., Ilhan, Z. and Orgun, E. (2019). comparison of some bacterial identification methods.Turkish J. Vet. Res. 3(1), 9-12.
Indramawan. (2014). Invertebrate Animals as Catfish Feed.Thesis. Faculty of Biology General
Sudirman University, Purwokerto.
Kresnawaty, I., Wahyu, R. and Sasongko, A. (2018). Amylase activity of amylolytic bacteria from Larvae black soldier fly (Hermetia illucens). Plantation Tower. 87(2), 140-146.
Madigan, MT, Martinko, JM, Dunlap, PV & Clark, DP 2006. Brock Biology of Microorganisms. 12th ed. San Francisco: Pearson Education.
Mangindaan, REP & Losung, F. (1991). Hemolytic activity of sea cucumbers (Bohadschia graeffei) from Malalayang coast, North Sulawesi at various temperatures and pHs. Sci. J. Sci. 13(1), 27–33.
Pamaya, D., Muchlissin, SI, Maharani, ETW, Darmawati, S. and Ethica, SN. (2018). Isolation
bacteria producing the protease enzyme Bacillus amyloliquefa irod2 on red oncom after 48 hours of fermentation. Edusaintek National Seminar. Semarang, Indonesia.
Permatasari, DA, Witaningrum, AM, Wibisono, FJ and Effendi, MH. (2020). Detection and
prevalence of multidrug-resistant Klebsiella pneumonia strains isolated from poultry farms in Blitar, Indonesia. Biodiversity, 21(10), 4642-4647.
Suardana, IW, Dinarini, NMAA and Sukrama, IDM. (2021). Species identification Streptococci –
Hemolysis isolated from nasal and tonsillar pigs by bacitracin test. Udayana Vet. Bull. 13(1), 27-33.
DOI: https://doi.org/10.21157/ijtvbr.v9i2.44058
Article Metrics
Abstract view : 0 timesPDF - 0 times
Refbacks
- There are currently no refbacks.

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Indexed in:
Copyright© 2016 | ISSN: 2503-4715
Published by:
The Faculty of Veterinary Medicine of Syiah Kuala University
In cooperation with:
Center for Tropical Veterinary Studies of Syiah Kuala University
and Indonesian Veterinary Medical Association (PDHI)
Online Submissions & Guidelines | Editorial Policies | Contact | Statistics
IJTVBR is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.