Production of Quinone Enzyme from Nicotiana tabacum Cell Culture Using Immobilized Dry Plant Foam

Teuku Maimun, Mirna Rahmah Lubis, Komala Pontas, Anwar Thaib, Abubakar Abubakar

Abstract


A study of immobilization of Nicotiana tabacum cell culture to increase the concentration of the quinone enzyme CoQ10 has been carried out. The immobilization medium was in the form of dry plant foam (dry ridge gourd). Cell growth and concentration of quinone enzyme were measured by fresh weight of the cell and High-Performance Liquid Chromatography methods, respectively. Immobilization of the Nicotiana tabacum plant cell culture was effective for cell growth, and increase CoQ10 concentration. CoQ10 concentration from the immobilized cell culture of the Nicotiana tabacum plant was 0.060 mg/g fresh cell weight after seven days of cultivation. The optimum temperature for the concentration of CoQ10 enzyme was 35°C.

Full Text:

PDF

References


Yulia, R., H. Putrizalda, A. Afiah, R. Armiliandi, S.R. Pinta, and L. Advinda, “Propagation of tobacco (Nicotiana tabacum) using a combination of IAA and BAP tissue culture techniques”, Prosiding Semnas Bio 2022, 2022, Jakarta.

Munir, A.A., M. Triatmasari, M.L. Arif, “Response of Madura rajan tobacco plant (Nicotiana tabacum L.) Prancak-N2 variety to NPK fertilizer dosage”, Rekayasa, vol. 3, no. 1, p. 29–35, 2010.

Djumali and S. Mulyaningsih, “Soil moisture effects on agronomic characters, yield and nicotine content of Temanggung tobacco (Nicotiana tabacum L; Solonaceae) at three types of soil”, Berita Biologi, vol. 13, no. 1, p. 1–11, 2014.

Smetanska, I., “Production of secondary metabolites using plant cell cultures”, Adv Biochem Engin/Biotechnol, vol. 2008, no. 111, p. 187–228, 2008.

Rao, S.R. and G.A. Ravishankar, “Plant cell cultures: chemical factories of secondary metabolites”, Biotechnology Advances, vol. 20, no. 2002, p. 101–153, 2002.

Banthorpe, D.V., “Secondary metabolism in plant tissue culture: scope and limitations”, Natural Product Reports, 1994.

Andrew, A. and H. Unno, Cultivation of Tobacco Cell Culture”, 1992.

Ikeda, T., “Selection of cultured tabasco cell strains producing high level of ubiquinone 10 by a cell cloning technique”, Agricultural Biological Chemistry, vol 45, no. 7, p. 1627–1633, 1981.

Clark, William, and Teruya, “Ubiquinone biosynthesis in Saccharomyces cerevisae”, The Journal of Biological Chemistry, vol. 266, no. 25, p. 16636–16644, 1991.

Bolton, J.L. and T. Dunlap, “Formation and biological targets of quinones: cytotoxic versus cytoprotective effects”, Chemical Research in Toxicology, vol. 2017, no. 30, p. 13–37, 2017.

Ho, Kelly, and Gregory, “Cell damage and oxygen mass transfer during cultivation of Nicotiana tabacum in stirred tank bioreactor”, Biotechnology Progress, vol.11, no. 2, 1995.

Halder, M. and Jha, S., “Morphogenesis, genetic stability, and secondary metabolite production in untransformed and transformed cultures”, Plant Cell and Tissue Differentiation and Secondary Metabolites, p. 1–60, 2020.

Huzaimi, A. and Mukhlishien, “Pengaruh dan ultraviolet pada kultivasi kultur sel tumbuhan tembakau dari Dataran Tinggi Gayo”, Proceeding Seminar Science and Technology, 1997, Padang.

Huzaimi, A., X.X. Hui, S. Yamamoto, H. Unno, “Produksi enzim quinone pada kultivasi sel tumbuhan tembakau, Proceeding Seminar Science and Technology, 1996, Medan.

Husin, M.A.H., M. Hasan, R.M. Taha, “Callus induction from tobacco (Nicotiana tabacum) leaf explants for the production of quinone”, Dev. Chem. Eng. Process, vol. 13, no. 5/6, p. 563–572, 2005.

Nurdin, S. and Huzaim, A. “Use of ascorbic acid for production of coenzyme quinone by induction of Nicotiana tabaccum cells”, Reaktor, vol. 5, no. 1, p. 31–34, 2001.

Bottacin, A. and G. Cacco, “Glucose repression and camp effect on enzyme activities in callus tissue of Nicotiana plumbaginifolia”, Plant Cell Biotechnology, Springer-Verlag Berlin Heidelberg, 1988.

Zulfikar, T.M., “Cell induction process of tobacco leaves (Nicotiana tabacum)”, Jurnal Nasional Komputasi dan Teknologi Informasi, vol. 4, no. 1, p. 75–80, 2021.

Sparla, F., G. Tedeschi, and P. Trost, “NAD(P)H: (quinone-acceptor) oxidoreductase of tobacco leaves is a Flavin mononucleotide-containing flavoenzyme”, Plant Physiol, vol. 1996, no. 112, p. 249–258, 1996.

Ikeda, T., “Formation of ubiquinone by tobacco plant cell and suspension culture, Agricultural Biological Chemistry, vol. 15, p. 568–569, 1976.


Article Metrics

Abstract view : 0 times
PDF - 0 times

Refbacks

  • There are currently no refbacks.