POTENSI EKSTRAK ALGA MERAH Gracilaria verrucosa SEBAGAI PENGHAMBAT PERKEMBANGAN PEMBENTUKAN BIOFILM Enterococcus faecalis PADA INFEKSI SALURAN AKAR GIGI

Abstract

Enterococcus faecalis (E. faecalis) is a facultative anaerobic Gram-positive bacteria which becomes
the main bacteria occurrence failure of endodontic treatment. The red algae Gracilaria verrucosa (G.
verrucosa) is one of the herbal plants that contain antibacterial compounds such as alkaloid,
flavonoid, saponin, steroid, and triterpenoid. This study aims to determine the potency red algae G.
verrucosa extract as inhibitor of E. faecalis biofilm formation development. The red algae G.
verrucosa extract with a concentration of 100%, 75%, 25%, 12,5%, and 6,25% was made in
maceration method using ethanol 96%. Microtiter Plate Biofilm Assay method and ELISA reader
with wavelength 595nm were used to test the biofilm. The result of One Way ANOVA showed
p<0,05 which mean there was an effect of the test group on the formation of E. faecalis biofilm
development and Post Hoc Tests-Duncan showed that there was a significant differences between
each group of those concentration. In the treatment group, there was found two concentration that
have significant differences, there were 100% and 6,25%. Based on the result of this study, we can
conclude that the red algae G. verrucosa extract was useful as an inhibitor of E. faecalis biofilm
formation development. The higher concentration of the red algae G. verrucosa, the higher its ability
as an inhibitor of E. faecalis biofilm formation development.

Keywords : Enterococcus faecalis, red algae Gracilaria verrucosa, Microtiter Plate Biofilm Assay
method, ELISA reader.