PENGARUH AIR PERASAN JERUK NIPIS (Citrus aurantifolia) TERHADAP PERTUMBUHAN BAKTERI Lactobacillus acidophilus

Abstract

Lactobacillus acidophilus is bacteria that has a role play in the caries process. Efforts to control
Lactobacillus acidophilus are using antibacterial agents from nature, one of these is lime juice (Citrus
aurantifolia). Lime juice has citric acid, flavonoids, saponins, fenols, tannins, alkaloids, and vitamin C that
have different antimicrobial action. The purpose of this study was to determine the effect of lime juice on
growth of Lactobacillus acidophilus. The research is a laboratory experimental. Antibacterial activity test
was performed using disc diffusion method consisted of 6 treatments and 3 times repetition. MRS-A is
poured into a petri dish and inoculated suspension of Lactobacillus acidophilus, then 6 paper disc that has
soaked into the lime juice concentration of 12.5, 25, 50, 100%, positive control, and negative control
placed on an agar medium has cooled. Then the plates were incubated at 37°C for 24 hours. Inhibition
zone of microbial growth were measured and recorded after incubation period. These results showed that
lime juice producing growth inhibition zone against Lactobacillus acidophilus on concentration 12,5% is
2,2 mm, concentration 25% is 2,3 mm, concentration 50% is 2,4 mm, and concentration 100% is 3,3
mm.The statistic calculation result by using One Way ANOVA is p=0,000 with significance p<0,05 The
conclusion of this study is the lime juice has weak inhibitory effect on the growth of Lactobacillus
acidophilus.

Keywords: Lime juice, caries, Lactobacillus acidophilus, disc diffusion method.