PENGARUH PERENDAMAN MODEL GIPSUM TIPE III DALAM LARUTAN DESINFEKTAN TERHADAP JUMLAH Pseudomonas aeruginosa

Abstract

Impression making is the main requirement to do dentures. Dental impressions become contaminated
with the patient’s microorganisms from patient’s saliva, blood, pus and debris which are known as
opportunistic patogens that can cross infect from the dental impressions to the gypsum casts poured
against them. This cross infection of microorganism remains, even after rinsing and desinfecting the
gypsum casts by immersing the gypsum cast in desinfeting solutions, such as glutaraldehyde 2% or
sodium hypoclorite 0,5% solutions, can be one of the way to prevent the potential of cross infection
during dental procedures the purpose of this study is to evaluate the effect of immersing gypsum casts
type III with the amount of Pseudomonas aeruginosa and the value of the surface hardness of gypsum
casts type III. The sample of this study is basedon the spesification ANSI/ADA No. 25, with three groups
of activity, with 9 samples each, which is, rinsing with distilled water (group A), immersion in
glutaraldehyde 2% (group B) and immersion in sodium hypoclorite 0,5% (group C), so that total
samples are 54 samples. Analisys with kruskal wallis and one way Anova. The number and standard
deviation of Pseudomonas aeruginosa in group A (222 ± 74.11 CFU / mL), group B and C (0 CFU /
mL). The value and standard deviation of surface hardness in group A (23.83 ± 0.69 VHN) , group B
(12.60 ± 0.72 VHN) and C (17.24 ± 1.14 VHN). There was an effect of immersion of gypsum type III
with glutaraldehyde 2% and sodium hypochlorite 0.5% on the amount of Pseudomonas aeruginosa and
surface hardness in the fabricating of dentures (p = 0.0001).
Keyword: Glutaraldehyde 2%, Sodium hypochlorite 0,5%, Pseudomonas aeruginosa, Surface
hardness.
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