The effect of ethanol extract of kirinyuh leaf (Chromolaena odorata L) on the allergy healing of rabbit (lepus curpaneums)

Abstract

Allergic rhinitis is one of the world's most common diseases and remains alive. The following diseases
are often found in otorhinolaryngology and constitute a significant health problem worldwide. Symptoms of nasal
hyperactivity and hyperresponsiveness are caused by the role of eosinophils, basophils, neutrophils, and other
inflammatory mediators. The development of medicinal plants in other countries is overgrowing due to many
adverse drug reactions. Chromolaena odorata (L) has been used as a traditional medicine because it has
ethnopharmacology effects such as alkaloids, essential oils, phenolics, flavonoids, steroids, phenolic compounds,
glycosides, phytates, saponins and tannins which are suppress allergenic. To determine the effect of ethanol extract
ointment of kirinyuh leaves (Chromolaena odorata (L)) on the levels of eosinophils, basophils, and neutrophils of
allergic that induced in a rabbit nasal mucosa. Experimental research with a pretest-posttest control group design
uses rabbits as a research subject. The extract of ethanol ointment from Chromolaena odorata (L) leaf can reduce the
nasal mucosa's allergen response. One-way ANOVA statistical analysis showed no significant difference in the
number of inflammatory cells between basophils, eosinophils, and neutrophils (p>0.05;1.00). Furthermore, based on
the analysis of the Kruskal Wallis Test, it showed that there were differences in the number of inflammatory cells
based on the concentration of the test material, related to the allergen response (p<0.05; 0.024) with a strong
relationship (r=0.907). Extracting ethanol ointment from Chromolaena odorata (L) leaf, which is applied topically,
can reduce allergic reactions and inflammation.
KEYWORDS: allergic rhinitis, eosinophils, basophils, neutrophils, Chromolaena odorata (L)