Antibacterial effect of Peronema canescens jack on the Staphylococcus aureus on the surface of acrylic resin

Okmes Fadriyanti; Yulia Rahmad; Muhammad Alif Gemilang

doi:10.24815/jds.v9i2.37526

Antibacterial effect of Peronema canescens jack on the Staphylococcus aureus on the surface of acrylic resin

Okmes Fadriyanti, Yulia Rahmad, Muhammad Alif Gemilang

Abstract

Background: Removable dentures made from Polymethyl Methacrylate (PMMA) are porous and can absorb saliva, leading to the attachment of microorganisms, including Staphylococcus aureus, which can trigger denture stomatitis. Preventing this condition involves using denture-cleaning solutions. Sodium hypochlorite is commonly used but can damage acrylic resin. An alternative is using natural ingredients, such as P. canescens jack, known for its antibacterial properties. Objective: This study evaluated the antibacterial effects of different P. canescens jack extract fractionation against Staphylococcus aureus on hot-polymerized acrylic resin. Methods: Extracts were fractionated using ethanol, ethyl acetate, and n-Hexane solvents. Acrylic resin plates (10x10x1 mm) were hot-polymerized and contaminated with Staphylococcus aureus, with 25 samples divided into five treatment groups: ethanol, ethyl acetate, n-Hexane, and a control group. The antibacterial activity was tested using a spectrophotometer and analyzed with One-way ANOVA and LSD tests to identify significant differences. Results: The study found a significant effect of P. canescens jack extract fractionation (p < 0.05) across different solvents. The n-Hexane solvent at 1% concentration exhibited the highest growth inhibition, with a value of 0.015 nm, compared to other solvents. Conclusion: Fractionation of P. canescens jack extract using n-Hexane as a solvent showed superior antibacterial activity against Staphylococcus aureus compared to sodium hypochlorite, making it a better option for preventing denture stomatitis

Keywords

Acrylic resin, fractionation, P. Canescens jack, Staphylococcus aureus

Full Text:

PDF PDF

References

Kesehatan BPdP. Laporan Nasional Riskesdas 2018. Jakarta: Badan Kebijakan Pembangunan Kesehatan; 2018.

Thio TLM. Pola Kehilangan Gigi dan Kebutuhan Perawatan Gigi Tiruan Sebagian Lepasan Dikelurahan Meras Kecamatan. e-GiGi 2014;2(1).

Puspitasari D, Saputera D, Anisyah RN. Perbandingan Kekerasan Resin Akrilik Tipe Heat Cured Pada Perendaman Larutan Desinfektan Alkalin Peroksida Dengan 75% Ekstrak Seledri (Apium Graveolens L.). 2016 2016;3(1):8.

Ajeng Erlinda M, Widaningsih, Anindita A. Effect Of Immersion Resin Acrylic Heat Cured On Sargassum ilicifolium as a Denture Cleanser Towards To Hardness Surface. DENTA 2021;12(1):1-8.

Okmes F, Irza PF, Sang SL. Perbedaan Kekasaran Permukaan Resin Akrilik yang Direndam dalam Larutan Sodium Hipoklorit dan Ekstrak Jamur Endofit Aspergillus Sp (Akar Rhizophora Mucronata). Jurnal Kedokteran Gigi Universitas Baiturrahmah 2018;5(2):153-61.

Laili AZ, Helmin E, Arif Y. Pengaruh Ekstrak Kloroform dan Etil Asetat Salvadora Persica Terhadap Zona Hambat Staphylococcus Aureus dan Streptococcus Mutans. Jurnal Kedokteran Komunitas (Journal of Community Medicine) 2021;9(1).

Indah R, Okmes F, Edrizal E. Efektivitas Pembersih Gigi Tiruan Dengan Rebusan Daun Sirih 25% dan 50% Terhadap Pertumbuhan Candida Albicans Pada Lempeng Resin Akrilik Polimerisasi Panas. Jurnal Kedokteran Gigi Universitas Baiturrahmah 2014;1(2):142-49.

Zulkarnain M, B JD. Pengaruh Perendaman Basis Gigi Tiruan Resin Akrilik Polimerisasi Panas dalam Larutan Sodium Hipoklorit dan Vinegar Cuka Putih Terhadap Kekasaran Permukaan dan Stabilitas Warna. Jurnal Material Kedokteran Gigi 2014(1):22-32%V 3.

Correia-Sousa J, Tabaio AM, Silva A, et al. The effect of water and sodium hypochlorite disinfection on alginate impressions. Revista Portuguesa de Estomatologia, Medicina Dentária e Cirurgia Maxilofacial 2013;54(1):8-12.

Pahuja RK, Garg S, Bansal S, Dang RH. Effect of denture cleansers on surface hardness of resilient denture liners at various time intervals- an in vitro study. J Adv Prosthodont 2013;5(3):270-7.

Liana R, Sri F, Poppy A, Rizki D. PPengaruh Konsentrasi Larutan Sodium Hipoklorit Sebagai Desinfektan Terhadap Kekuatan Impak Basis Gigi Tiruan Resin Akrilik: Effect of Concentration Sodium Hypochlorite Solution As Desinfectan to The Impact Strength of Denture Base Acrylic Resin. Dentika: Dental Journal 2014;18(1):27.

Javed A, Annu Kumari MN, SivaKumar Medam. Evaluation of the Combinational Antimicrobial Effect of Annona Squamosa and Phoenix Dactylifera Seeds Methanolic Extract on Standard Microbial Strains. International Research Journal of Biological Sciences 2013;2:68-73.

Laksmi Aldelina N, Sandra Sari D, Amin MN. Efek pemberian ekstrak daun pepaya muda (Carica papaya) terhadap jumlah sel makrofag pada gingiva tikus Wistar yang diinduksi Porphyromonas gingivalis. Artikel Penelitian Mahasiswa UNEJ 2013.

Latief M, Tarigan IL, Sari PM, Aurora FE. Aktivitas Antihiperurisemia Ekstrak Etanol Daun Sungkai (Peronema canescens Jack) Pada Mencit Putih Jantan. Pharmacon: Jurnal Farmasi Indonesia 2021;18(1):15.

Wahyudi W, Muttaqin Z, Mojiol AR. Analisis Pertumbuhan dan Hasil Tanaman Sungkai (Peronema Canescens Jack.) di Kalimantan. Jurnal Sains Natural 2017;2:116.

Rahman A, Rengganis GP, Prayuni S, et al. The Effect of Sungkai Leaves (Peronema Canescens) Infusion on The Number of Leukocytes in Mice. Journal of Healthcare Technology and Medicine 2022;7(2):614-20.

Pindan NP, - D, Saleh C, Magdaleni AR. Uji Fitokimia dan Uji Aktivitas Antioksidan Ekstrak Fraksi N-Heksana, Etil Asetat dan Etanol Sisa Dari Daun Sungkai (Peronema Canescens Jack.) dengan Metode DPPH. JURNAL ATOMIK 2021(1):22-27.

Okmes F, Valendriyani N, Afnia ZU. The Spray Effect of Peronema Canescens Jack As a Disinfectant Against the Growth of Staphylococcus aureus on the Surface of Alginate Molds. International Journal of Innovative Science and Research Technology 2022;7(7):126-29.

Ibrahim A, Utami IW, Agustina R. Aktivitas Sediaan Gel Antiseptik Tangan Berbahan Aktif Ekstrak Fraksi Etanol Daun Sungkai (Peronema canencens Jack.) terhadap Beberapa Bakteri Patogen. Journal of Tropical Pharmacy and Chemistry 2015;3(2):94-100.

Purba AUC, Naliani S, Sugiaman VK. Efektivitas Antibakteri Fraksi Buah Merah (Pandanus conoideus Lam) sebagai Pembersih Gigi Tiruan Sebagian Lepasan terhadap Staphylococcus aureus. e-GiGi 2023;11(2):143-51.

Dita P, Rikhsan K, Rahmawati R. Uji Aktivitas Antibakteri Fraksi Metanol, Etil Asetat, dan N-Heksana Rimpang Lengkuas Merah (Alpinia purpurata, K. Schum) terhadap Bakteri Propionibacterium acnes. Protobiont: Jurnal Elektronik Biologi 2023;12(1).

Haeria H, Nursyamsi D, Risnawati H. Antibacterial Activity of Bidara Leaf Fractions (Ziziphus mauritiana). Ad-Dawaa Journal of Pharmaceutical Sciences 2018;1(2).

Loizzo MR, Tundis R, Bonesi M, et al. Evaluation of Citrus aurantifolia peel and leaves extracts for their chemical composition, antioxidant and anti-cholinesterase activities. J Sci Food Agric 2012;92(15):2960–67.

Dyah W, Fikria KI, Endang S. Efek Antibakteri Sodium Hypochlorite terhadap Staphylococcus aureus Antibacterial Effect of Sodium Hypochlorite to Staphylococcus aureus. Jurnal Ilmiah Kesehatan Masyarakat : Media Komunikasi Komunitas Kesehatan Masyarakat 2019;11(4):302-07.

Ajithan L, Jayakumar A, Chandrathara, Nair C. The Relevance of Polishing Acrylic Dentures -An Overview. Acta Scientific Dental Sciences 2021;5(5):63-70.

Kusriani RH. Uji Aktivitas Antibakteri Ekstrak Dan Fraksi Kulit Batang Dan Daun Sungkai (Peronema Canescens Jack) Terhadap Staphylococcus Aureus Atcc 25923 Dan Escherichia Coli ATCC 25922. JURNAL FARMASI GALENIKA 2015;2(1):1-8.

Amalia S, Wahdaningsih S, Untari EK. Antibacterial Activity Testing of N-hexane Fraction of Red Dragon (Hylocereus Polyrhizus Britton & Rose) Fruit Peel on Staphylococcus Aureus Atcc 25923. 2015 2015;19(2):89-94.

Sudik WA, Nisa SC, DA RR, ZA BMa. Aktivitas Antibakteri Ekstrak Etanol 96% Buah Blewah (Cucumis melo L. var. cantalupensis) terhadap pertumbuhan bakteri Escherichia coli. Pharmaceutical Journal of Indonesia 2019;5(1):61-66.

Nomer N, Duniaji A, Nocianitri K. Flavonoid and Anthocyanin Analysis of Sappan Wood Extract (Caesalpinia sappan L.) and Antibacterial Activity Against Vibrio cholerae Jurnal Ilmu dan Teknologi Pangan (ITEPA) 2019;8(2):216-25.

Egra S, Mardhiana M, Rofin M, et al. Aktivitas Antimikroba Ekstrak Bakau (Rhizophora mucronata) dalam Menghambat Pertumbuhan Ralstonia solanacearum Penyebab Penyakit Layu. 2019 2019;12(1):6.

DOI: https://doi.org/10.24815/jds.v9i2.37526