LIVABILITY AND RECOVERY RATE OF BALI CATTLE SPERMATOZOA DURING PRESERVATION IN TRIS-BASED EGG YOLK DILUENT WITH DIFFERENT SUCROSE LEVELS

Yendraliza Yendraliza, Tania Tania, Restu Misrianti, Zumarni Zumarni

Abstract


This study aimed to determine the effect of sucrose addition in tris-based egg yolk diluent in maintaining and protecting spermatozoa during preservation. The design of this study was a completely randomized design (CRD) which consisted of five treatments with ten replications. The treatments were tris-based egg yolk diluent without sucrose (P0, control), tris-based egg yolk diluent with 0.2% sucrose (P1), tris-based egg yolk diluent with 0.3% sucrose (P2), tris-based egg yolk diluent with 0.4% sucrose (P3) and tris-based egg yolk diluent with 0.5% sucrose (P4). The parameters measured were motility, livability, abnormality, intact plasma membrane, and recovery rate. Semen was evaluated 2 times, freshly after being collected and post thawed. The results showed that the increase level of sucrose addition from 0.2% to 0.5% increased the motility value from 43.59% to 48.15%, the livability value from 51.24% to 55.45%, the intact plasma membrane value from 44.66% to 48.21%, the recovery rate value from 54.6% to 60.2 %, and reduce the abnormality value from 13.49%-10.24%. It can be concluded that the addition of 0.2- 0.5% sucrose in tris-based egg yolk diluent could increase motility, livability, intact plasma membrane, recovery rate, and could reduce the abnormalities of Bali cattle spermatozoa during preservation

Keywords


abnormalitas, livabilitas, motilitas, membrane plasma utuh.

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Akhter, S., B.A. Rakha, M.S. Ansari, A.U. Husna, S. Iqbal, M. Khalid. 2016. Evaluation of quail and turkey egg yolk for cryopreservation of nili-ravi buffalo bull semen. Theriogenology. 1-7.

Aisen. E.G., H.L. Alvarez. and A. Venturino. 2002. Cryopreservation and post thawed fertility of ram semen frozen in different trehalose concentration. Theriogenology. 57: 1801-1808.

Arifiantini, R.I., B. Purwantara. 2010. Motility and viability of friesian Holstein spermatozoa in three different extender stored at 5 °C. J Indonesian Trop Anim Agric. 35(4):222-226.

Anwar, P., Y. S. Ondho Dan D. Samsudewa 2014. Pengaruh Pengencer Ekstrak Air Tebu Dengan Penambahan Kuning Telur Terhadap Kualitas Spermatozoa Sapi Bali. Jurnal Peternakan. 11 (2): 48 – 58.

Bakas, L.S. And E.A. Disalvo. 1991. Effects Of Ca2+ On The Cryoprotective Action Of Trehalose. Cryobiology. 28: 347-353.

Barbonetti, A., M.R.C.Vassallo, D. Fortunato, S. Francavilla, M. Maccarrone, F. Francavilla. 2010. Energetic metabolism and human sperm motility: impact of CB1 receptor activation. Endocrinology. 151: 5882-5892

Bergeron, A., Y. Brindle, P. Blondin, P. Munjanath. 2007. Milk casein decrease the binding of the major bovine seminal plasma protein to sperm and prevent lipid loss from the sperm membrane during sperm storage. Biol Reprod. 77:120-126.

Bearden, J.H., J.W. Fuquay, S.T. Willard. 2004. Applied Animal Reproduction 6th Ed. New Jersey (US): Pearson Education Inc.

Colenbrander, B., A.R. Fazeli, A. van Buiten, J. Parlevliet, B.M. Gadella.

Assesment of sperm cell membrane integrity in the horse. Acta Vet Scand. 88: 49-58.

Direktoral Jenderal Peternakan. 2000. Statistik Peternakan Indonesia. Direktorat Jenderal Peternakan, Jakarta.

El-Sheshtawy, R.I., W.S. El-Nattat. 2018. Effect of tris-extender supplemented with various concentrations of strawberry (Fragaria spp.) on bull semen preservability. Asian Pasifik Journal of Reproduction. 7 (2);93-96

Garner, D.L., and E.S.E. Hafez. 2016. Spermatozoa and Seminal Plasma. In Reproduction in Farm Animal eds. 7th. Lippincott & Williams.Baltimore, Marryland, USA.

Hafez, E. S. 2016. Reproduction In Farm Animals Ed. Lea And Febiger.Philadelpia.

Hammersted, R. 1993. Maintenance of Bioenergetic in Sperm and Prevention of Lipid Peroxidation. M.J.D’occhio. Australia.

Herdis, I. W. A. Darmawan, dan M. Rizal. 2016. Penambahan beberapa jenis gula dapat meningkatkan kualitas spermatozoa beku asal epididimis ternak Domba. Jurnal Kedokteran Hewan. 10 (2):200-204.

Holt, W.V. 2000. Basic aspects of frozen storage of semen. Anim Reprod Sci. 18(62):3-22.

Jeyendran, R.S., H. H. van der Ven, M. Perez-Pelaez, B.G. Crabo, and L.J.D. Zaneveld. 1984. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil., 70:219–228.

Jian, H. H., L. S. Zan., X. L. Zhao., Q. W. Li., Z. L. Jiang., Y. K. Li., and X. Li. 2010. Effects of trehalose supplementation on semen quality and oxidative stress variables in frozen thawed bovine semen. JAnim Sci. 88: 1657-1662.

Komariah, I. Arifiantini dan W. Nugraha. 2013. Kaji Banding Kualitas Spermatozoa Sapi Simmental, Limousin, dan Friesian Holstein Terhadap Proses Pembekuan. Buletin Peternakan. 37 (3): 143- 147

Lehninger, A.L. 1994. Dasar-dasar Biokimia Jilid 1. Alih bahasa: Thenawijaya M. Erlangga, Jakarta. Hlm 358-367.

Maxwell, W.M.C and S.Salamon. 2000. Storage of ram semen. J. of Anim. Reprod. Sci.62: 77-111.

Manjunath, P. 2012 New insight into the understanding of the mechanism of sperm protection by extender components. Anim Reprod. 9:809-815.

Meryman, H.T. 2007. Cryopreservation of living cells: principles and practice. Transfusion. 47:935-945

Murray, R.K., D.K. Gardner, P.A. Mayer And V.W. Rodwell. 1999. Biokimia Harper. Ed-24. Alih Bahasa Oleh Andry Hartono. Penerbit Buku Kedokteran Egc,Jakarta.

Nainga, S.W., H. Wahida., K. M. Azamc., Y. Rosninaa., A.B. Zukib., S. Kazhala., M.M. Bukara., M. Theind, T. and , M.M. S. Kyawe. 2010. Effect of sugars on characteristics of Boer goat semen after Cryopreservation. Animal Reproduction Science.122: 23–28.

Nicollajsen H, A. Hvidt. 1994. Phase behaviour of the system trehalose NaCl water. Cryobiology. 31:199--205.

Parks JE, Graham JK. 1992. Effects of cryopreservation procedures on sperm membranes. Theriogenology. 38: 209-222.

Rizal, M., M. Riyadhi, B. Irawan, A. Wahdi, Habibah, Herdis. 2017. Daya Hidup Spermatozoa Epididimis Sapi Persilangan yang Dipreservasi dengan Air Kelapa Muda pada Suhu 5oC. Jurnal Veteriner. 18 (4) : 571-579.

Rizal, M., M. Riyadhi. 2016. Fertilitas Semen Kerbau Rawa (Bubalus bubalis carabanensis) yang Diencerkan dengan Pengencer Nira Aren. Jurnal Veteriner. 17(3); 457-467

Rosadi, B., T. Sumarsono dan Darmawan. 2015. Motilitas Spermatozoa Kerbau Lumpur pada Penyimpanan Suhu Beku dalam Es. Jurnal-Jurnal Ilmu Peternakan XVIII. 2: 98-101

Shukla, M.K. 2011. Applied Veterinary Andrology and Frozen Semen Technology. Pitam Pura. New Delhi India.

Solihati, N., S. D. Rasad, R. Setiawan, S. Nurjanah. 2018. Pengaruh kadar gliserol terhadap kualitas semen domba lokal. Jurnal Biodjati. 3 (1): 63-71

Susilawati, T., D.Ratnawati, N. Isnaini, Kuswati, and A. P. Yekti, 2018. character of liquid semen motility in various diluents on balinese cattle during cold storage. asian Journal. of Microbiol. Biotech. Env. Sc. 20(1): 166-172

SNI (Standar Nasional Indonesia). (2017). Semen Beku - Bagian 1: Sapi (SNI 4869- 1:2017). BSN (Badan Standarisasi Nasional). Jakarta

Steel RGD, Torrie JH. 1993. Prinsip dan Prosedur Statistika (Pendekatan Biometrik). Sumantri B, penerjemah. Jakarta (ID): Gramedia Pustaka Utama.

Toelihere, M. R. 1993. Inseminasi Buatan Pada Ternak. Angkasa. Bandung.

Uchida, T., M. Nagayama, T. Shibayama and K. Gohara. 2007.

Morphological investigations of disaccharide molecules for growth inhibition of ice crystals. J Cryst Growth. 299 (1):125-135.

Vishwanath, R., P. Shanon. 2000. Storage of bovine in liquid and frozen state. Anim Reprod Sci. 62:23-53.

Woelders, H., A. Matthij, B. Engel. 1997. Effect of trehalose and sucrose, osmolality of the freezing medium, and cooling rate on viability and intactness of bull sperm after freezing and thawing. Cryobiol. 35: 93-105.

Yendraliza, P. Anwar dan Rodiallah. 2015. Bioteknologi Reproduksi. Aswaja Pressindo. Yogyakarta

Yulnawati dan Herdis. 2009. Kualitas semen cair domba garut pada penambahan Sukrosa dalam pengencer tris kuning telur. JITV. 14(1):45-49

Yulnawati, Herdis, H. Maheshwari, M. Rizal. 2008. Kualitas spermatozoa epididymis kerbau belang pada penambahan raffinosa sebagai krioprotektan ekstraseluler. Jurnal Ilmu Ternak dan Veteriner. 13: 30-34.

Yulnawati, M. Gunawan, Herdis, H. Maheshwari dan M. Rizal. 2009. Peranan gula sebagai krioprotektan ekstraseluler dalam mempertahankan kualitas semen beku kerbau lumpur. Prosiding Seminar Nasional Potensi dan Pengembangan Peternakan Maluku dalam Mendukung Ketahanan Pangan Nasional. Ambon : 236-250.

Zelpina, E., B. Rosadi dan T. Sumarsono. 2012. Kualitas Spermatozoa Post Thawing dari Semen Beku Sapi Perah. Jurnal Ilmu-Ilmu Peternakan. 25(2) : 94-102




DOI: https://doi.org/10.21157/j.ked.hewan.v13i2.13033

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