LIVABILITY AND RECOVERY RATE OF BALI CATTLE SPERMATOZOA DURING PRESERVATION IN TRIS-BASED EGG YOLK DILUENT WITH DIFFERENT SUCROSE LEVELS

Yendraliza Yendraliza, Tania Tania, Restu Misrianti, Zumarni Zumarni

Abstract


This study aimed to determine the effect of sucrose addition in tris-based egg yolk diluent in maintaining and protecting spermatozoa during preservation. The design of this study was a completely randomized design (CRD) which consisted of five treatments with ten replications. The treatments were tris-based egg yolk diluent without sucrose (P0, control), tris-based egg yolk diluent with 0.2% sucrose (P1), tris-based egg yolk diluent with 0.3% sucrose (P2), tris-based egg yolk diluent with 0.4% sucrose (P3) and tris-based egg yolk diluent with 0.5% sucrose (P4). The parameters measured were motility, livability, abnormality, intact plasma membrane, and recovery rate. Semen was evaluated 2 times, freshly after being collected and post thawed. The results showed that the increase level of sucrose addition from 0.2% to 0.5% increased the motility value from 43.59% to 48.15%, the livability value from 51.24% to 55.45%, the intact plasma membrane value from 44.66% to 48.21%, the recovery rate value from 54.6% to 60.2 %, and reduce the abnormality value from 13.49%-10.24%. It can be concluded that the addition of 0.2- 0.5% sucrose in tris-based egg yolk diluent could increase motility, livability, intact plasma membrane, recovery rate, and could reduce the abnormalities of Bali cattle spermatozoa during preservation

Keywords


abnormalitas, livabilitas, motilitas, membrane plasma utuh.

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DOI: https://doi.org/10.21157/j.ked.hewan.v13i2.13033

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