BODY WEIGHT, MUSCLE WIGHT, PROTEIN, DNA AND RNA CONTENTS IN BREAST MUSCLE (M. Pectoralis Major) OF SELECTED LOCAL MEAT CHICKEN FED ON A DIFFERENT LEVEL OF KIAPU (Pistia stratiotes L.) IN FERMENTED DIET

Abstract

The study to evaluate changes in body weight, muscle weight, muscle protein content and total DNA and RNA on breast muscle of selected
local chicken due to different levels of fermented feed containing Kiapu (Pistia stratiotes L.) was conducted using a factorial randomized design
for 7 weeks. This study used 72 selected local chickens, which were separated into 2 groups (male and female) and fed on 3 types of feed
treatment, namely P0 (control treatment with 100% commercial feed), P1 (80% commercial feed + 20% fermented Kiapu feed), and P2 (70%
commercial feed + 30% fermented Kiapu feed). Parameters were body weight, muscle weight, muscle protein content and total DNA and RNA
on breast muscle of chicken. Total protein, DNA and RNA content were analysed by enzymatic reactions of prokaryotic cell and measured with a
UV-Spectrophotometer according to the specified OD (optical density). The results showed that feeding Kiapu fermented 20-30% increased body
weight and protein content of breast muscle of female chicken by change in DNA content. However, the feeding treatment had no changes in
muscle weight and RNA content of both in male and female chicken. On the other hand, substitution of 20-30% Kiapu fermented feed caused
negative changes in DNA level of breast muscle. From this study, it was concluded that substitution of fermented feed containing 20-30% Kiapu
associated with the growth process of breast muscle protein is suitable for female but not suitable for male chicken.