EFFECT OF ETHANOL EXTRACT FROM MALACCA LEAVES (Phyllanthus emblica) ON THE SPERM QUALITY OF MICE

Cut Nila Thasmi, Nuzul Asmilia, Elfi Satria Suryani, Hafizuddin Hafizuddin, Mulyadi Adam, Arman Sayuti, Nazaruddin Nazaruddin, Budianto Panjaitan

Abstract


This study aims to analyze the effect of administering Malacca leaves (Phyllanthus emblica) extract on the sperm quality of mice. This leaf extract was administered orally using gavage method once daily for 21 days. There were four treatment groups: the control group (P0) received distilled water while the first (P1), second (P2), and third (P3) treatment groups received 300 mg, 600 mg, and 1200 mg of Malacca leaf ethanol extract/kg body weight, respectively. Each groups consisted of four mice and was evaluated for sperrm motility, viability, morphology, and concentration. The results showed that sperm motility, viability, morphology and sperm concentration tended to decrease after administration of Malacca leaf ethanol extract for all parameters, although there was no significant difference (P>0.05). It was concluded that the administration of Malacca leaf ethanol extract tends to reduce the sperm quality of mice. ___________________________________________________________

Keywords


Malacca leaves; mice; Phyllanthus emblica; sperm

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Akmal M, Siregar TN, Wahyuni S, Hamny, Nasution MK, Indriati W, Panjaitan B, Aliza D. 2016. The expression of cyclic adenosine monophosphate responsive element modulator in rat sertoli cells following seminal extract administration. Veterinary World. 9(9):1001-1005.

Alahmar AT, Calogero AE, Sengupta P, Dutta S. 2021. Coenzyme Q10 improves sperm parameters, oxidative stress markers and sperm DNA fragmentation in infertile patients with idiopathic oligoasthenozoospermia. The World Journal of Men's Health. 39(2):346-351.

Barbosa ADP. 2014. An overview on the biological and pharmacological activities of saponins. International Journal of Pharmacy and Pharmaceutical Sciences. 6(8):47-50.

Daud D, Dewa MSAM, Mahbob ENM, Razak WRWA. 2021. Short communication: Phytochemical diversity and bioactivity of Malaysian Premna cordifolia (Lamiaceae). Biodiversitas Journal of Biological Diversity. 22(6):3245-3248.

Elya B, Kusmana D, Krinalawaty N. 2010. Spermatozoa quality from Polyscias guilfoylei. Makara Journal of Science. 14(1):51-56.

Hafizuddin H, Karja NWK, Praharani L, Setiadi MA. 2020. Adiponectin and testosterone levels and its correlation with fertility in Anglo-Nubian x Etawah Grade crossbred bucks. Tropical Animal Science Journal. 43(2):110-116.

Hafizuddin H, Karja NWK, Praharani L, Setiadi MA. 2021. Breed and age effects on concentration of adiponectin and reproductive performance in Anglo Nubian, Etawah grade and its crossbred bucks. Biodiversitas Journal of Biological Diversity. 22(2):1112-1119.

Handayani N, Gofur A. 2017. The potency of pulutan (Urena lobata L.) leaves decoction as antifertility based on its effect on uterine development of balb c mice (Mus musculus). Jurnal Kedokteran Hewan. 11(4):153-155.

Khoiriyah U, Pasaribu N, Hannum S. 2015. Distribusi Phyllanthus emblica L. di Sumatera Utara bagian selatan. Majalah Ilmiah Biologi. 32(2):98-102.

Leeson R, Leeson T, Paparo A. 1996. Buku Ajar Histologi Edisi VII. Terjemahan Tambayong et al, EGC, Jakarta.

Lestari SW, Miati DN, Seoharso P, Sugiyanto R, Pujianto DA. 2017. Sperm Na+, K+-ATPase α4 and plasma membrane Ca2+-ATPase (PMCA) 4 regulation in asthenozoospermia. Systems Biology in Reproductive Medicine. 63(5):294-302.

Magedans YVdS, Phillips MA, Fett-Neto AG. 2021. Production of plant bioactive triterpenoid saponins: from metabolites to genes and back. Phytochemistry Reviews. 20(2):461-482.

Mulyani H, Widyastuti SH, Ekowati VI. 2016. Herbs as traditional medicine jamu of the disease in serat primbon jampi jawi jilid I. Jurnal Penelitian Humaniora. 21(2):73-91.

Murtini JT, Triwibowo R, Indriati N, Ariyani F. 2010. In-vivo subchronic toxicity test of Spirulina platensis. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan. 5(2):123-134.

Purwaningsih E. 1996. Morfologi spermatozoa adakah kaitannya dengan kehamilan. Jurnal Kedokteran YARSI. 4(1):54-65.

Purwoistri RF. 2010. Pengaruh ekstrak biji pepaya (Carica papaya L.) terhadap spermatogenesis dan tebal epitel tubulus seminiferus mencit (Mus musculus) Jantan. Universitas Islam Negeri Maulana Malik Ibrahim.

Robinson T. 2003. Kandungan organik tumbuhan tinggi. ITB, Bandung.

Setyowati E, Ariani D, Ashadi BM, Hidayat A. 2015. Aktivitas antifertilitas kontrasepsi dari kulit Durian (Durio zibethinus Murr.) varietas Petruk. Universitas Sebelas Maret, Surakarta.

Susetyarini E. 2011. Aktivitas dan Keamanan Senyawa Aktif Daun Beluntas Sebagai Antifertilitas Tikus Putih Jantan serta Pemanfaatannya untuk Penyusunan Buku Antifertilitas. Universitas Negeri Malang, Malang.

Susetyarini E. 2013. Beluntas leaf tannin activity against spermatozoa concentration white male rats. Jurnal Gamma. 8(2):14-20.

Sutapa GN, Supartha IW, Wijaya IN, Puja IK, Syaifudin M. 2020. The effectiveness of 60Co gamma-ray exposure to the reproductive systems of rat (Rattus argentiventer) as sterile male technique. Biodiversitas Journal of Biological Diversity. 21(8):3805-3810.

Syafruddin S, Iryandi F, Rahmi RASa, Husnurrizal H, Armansyah T, Panjaitan B, Sayuti A, Sutriana A, Aliza D, Hafizuddin H, et al. . 2020. The effect of gonadotropin-releasing hormone (GnRH) on semen quality and testosterone level of Nubian goats. Veterinarija ir Zootechnika. 77(99):16-21.

von Dippe PJ, Abraham A, Nelson CA, Smith WG. 1972. Kinetic and molecular properties of lysine-sensitive aspartokinase: quaternary structure, catalytic activity, and feedback control. Journal of Biological Chemistry. 247(8):2433-2438.

WHO. 2010. Laboratory manual for the examination and processing of human semen. World Health Organization, Geneva.

Zahrina AD. 2015. Uji Aktivitas Antifertilitas Ekstrak Etanol 96% Daun Sambiloto (Andrographis paniculata Nees.) Pada Tikus Jantan Galur Sprague-Dawley Secara In Vivo. Universitas Islam Negeri Syarif Hidayatullah, Jakarta.




DOI: https://doi.org/10.21157/j.ked.hewan.v16i3.27246

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