Goblet Cells Proliferation of Duodenum, Jejunum, and Ileum of Laying Hens Immunized with Protein of Excretory-Secretory of Ascaridia galli

Abstract

This research was conducted in order to examine the goblet cells proliferation in duodenum,
jejunum, and ileum of laying hens due to exposured with protein of excretory/secretory (ES) of Ascaridia
galli adult worm. Thirty heads of laying hens were divided in to two groups. The first group was treated
with 4,000 infective larva (L2) of A. galli and the second group was immunized with 380
µg of ES and
four hours later was challenged with 4000 L2. All treatments were given orally using stainless steel
canule directly to the oesophagus. Data was taken on the 3, 6, 9, 12, and 15 days post immunization
(p.i.). The goblet cells were determined by Periodic Acid Schiff (PAS) staining. The result showed that
immunization was able to increased goblet cells proliferation significantly at 12 and 15 day p.i. on the
duodenum, and at 9, 12, and 15 day p.i. on the jejunum, but goblet cells proliferation did not
significantly on the ileum. From this result we suggested that ES would beneficial in the strengthen the
host’s defence mechanisms in the intestinal mucosa.

Keywords: Ascaridia galli, excretory/secretory, goblet cells