IDENTIFICATION OF COPPER-INDUCIBLE GENES IN SWORDTAIL FISH (Xiphophorus spp.) USING DIFFERENTIAL DISPLAY

Abstract

Differential display technique was used to identify differentially expressed gene of

swordtail fish (Xiphophorus spp.) that induced by 1 µg/ml copper for 24 hours. From eight
primers that have been used for differential display, seven of them showed the differential
genes which were not present in the control. The reamplification of these fragments ranging
from below 100 to 200 bp. But only two fragments were successfully cloned and sequenced,
those are H-AP10 and H-AP11. Sequence analysis showed that both of the sequences revealed
high similarity with Homo sapiens stress-associated endoplasmic reticulum protein, a signal
generated that induces apoptosis.

Keywords: differential display, swordtail fish, copper pollution, mRNA expression, biomarker