EFEK SITOTOKSIK HAARLEM OIL TERHADAP HL-60 CELL LINE DAN Steinernema feltiae (Cytotoxic Effect of Haarlem Oil on HL-60 Cell Line and Steinernema feltiae)

Muhammad Bahi, Claus Jacob, Khairan Khairan

Abstract


The objective of this research was to investigate cytotoxicity effect of Haarlem oil (HO) on HL-60 cell line and Steinernema feltiae (S. feltiae). The test results using trypan blue method and CellTiter-Glo® assays revealed that HO showed higher cytotoxicity effect against HL-60 cell line especially at concentration of 1:10 and 1:5. Meanwhile, the MTT (3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay revealed that the higher cytotoxicity effect showed at 1:5, with the cell viabilities were around 40-65%. The nematicidal assay discovered that HO at concentration of 1:5 showed higher activities compare to other concentrations with percentage viability of 70%. These studies have shown that HO shows cytotoxic effect against HL-60 cell lines, and moderate activity against S. feltiae as well.

Keywords


Haarlem oil, HL-60 cell line; organosulfur compounds; Steinernema feltiae

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DOI: https://doi.org/10.21157/j.ked.hewan.v10i2.5038

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