MOLECULAR DETECTION OF GENE RESISTANT TO VARIOUS INSECTICIDES IN Aedes aegypti AT BANYUWANGI EAST JAVA USING POLYMERASE CHAIN REACTION

Aditya Yudhana, Ratih Novita Praja, Maya Nurwartanti Yunita

Abstract


The purpose of this study was to determine the type of insecticides which Aedes aegypti mosquitoes are resistant to, so that other susceptible
insecticides still can be used. The study was a cross sectional epidemiological study with cluster sampling in Sub-district Wongsorejo,
Banyuwangi, Muncar, Tegaldlimo, Kalibaru which is considered sufficient to represent Banyuwangi Regency. Mosquito samples appropriate to
the characteristics were isolated and tested for insecticide-resistance primer namely voltage gated sodium channel (VGSC) to determine the
specific resistance expressed in mosquitoes. The result of resistance test using WHO standard method showed that Aedes aegypti mosquito from
Banyuwangi Regency were resistant to cypermethrin 0.25% and malathion 0.8%. There was a VGSC coding gene with 250 bp band detected
using polymerase chain reaction (PCR) technique which was associated with the resistance of Aedes aegypti mosquitoes in Banyuwangi Regency
to organophosphate insecticides (malathione) and pyrethroid insecticides (cypermethrin).


Keywords


Aedes aegypti; insecticide; polymerase chain reaction; resistance

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References


Banyuwangi Health Office. 2015. Profil Kesehatan Kabupaten

Banyuwangi. Banyuwangi.

Brogdon, W.G. and J.C. McAllister. 1998. Insecticide resistance and

vector control. J. Emerg. Infect. Dis. 4(4):605-613.

David, A.W. and H.M. Gilles. 2002. Essential malariology. J.

Emerg. Infect. Dis. 3:159-166.

Hemingway, J. 1997. Insecticide resistance mechanisms and cross

resistance implications. J. Bio. 64(5):753-758.

Herath, P.R.J. 2012. Insecticide Resistance Status in Disease Vectors

and its Practical Implications. Intercountray Workshop on

Insecticide Resistance of Mosquito. Colombo, Sri Lanka

:751-761.

Kazanidou, A., D. Nikou, M. Gregoriou, J. Vontas, and G. Skavdis. 2009.

A multiplex PCR assay for simulaneous genoyping of kdr and ace-1

Loci in Anopheles gambiae. Am. J. Trop. Med. Hyg. 80(2):236-238.

Komalamisra, N., R. Srisawat, T. Phanbhuwong, and S. Oatwaree.

Insecticide susceptibility of the dengue vector, Aedes

aegypti (L.) in metropolitan Bangkok, Southeast Asian. J. Trop.

Med. Pub. Health. 42(4):814-823.

Najera, J.A. and M. Zaim. 2001. Malaria vector control.insecticide

for indoor residual spraying. J. Emerg. Infect. Dis. 1(3):36-47.

Sayono, D. Syafruddin, and D. Sumanto. 2012. Distribusi resistensi

nyamuk Aedes aegypti terhadap insektisida sipermetrin di

Semarang. J. Eko. Kes. 3(2):73-82.

Sinkins, S. 2010. Genome sequence of Aedes aegypti, a major

arbovirus vector. J. Sci. 3(16):1718-1723.

Soderlund, D.M. 2008. Pyrethroid, knockdown resistance and sodium

channels. Pest. Manag. Sci. 64:610-616.

Widiarti, B. Heriyanti, and D. Boewono. 2011. Peta resistrensi vektor

demam berdarah dengue Aedes aegypti terhadap insektisida

kelompok organo fosfat, karbamat, dan pyretroid di Provinsi

Jawa Tengah dan Daerah Istimewa Yogyakarta. J. Eko. Kes.

(2):93-111.

Wooden, J., S. Kyes, and C.H. Sibley. 1993. PCR and strain

identification in Plasmodium falciparum. J. Parasitol. 9:303-305.




DOI: https://doi.org/10.21157/j.ked.hewan.v12i2.7625

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