Effectiveness of Kersen Leaf Extract Cream (Muntingia calabura L.) on The 3rd Day of Incision Healing Process on The Skin of Streptozotocin Induced  White Rats (Rattus norvegicus)

M. Nur Salim; Nurul Sakinah Azhar; Dian Masyitha; Henni Vanda; Rosmaidar Rosmaidar; Zainuddin Zainuddin

doi:10.21157/j.med.vet..v18i2.40208

Effectiveness of Kersen Leaf Extract Cream (Muntingia calabura L.) on The 3rd Day of Incision Healing Process on The Skin of Streptozotocin Induced White Rats (Rattus norvegicus)

M. Nur Salim, Nurul Sakinah Azhar, Dian Masyitha, Henni Vanda, Rosmaidar Rosmaidar, Zainuddin Zainuddin

Abstract

A wound is a break in the continuity of tissue, which physiologically undergoes several phases of healing: hemostasis, inflammation, proliferation, and maturation. The wound healing phase can be prolonged if one experiences metabolic disorders such as diabetes mellitus (DM). Treatment is necessary to prevent the prolongation of the wound healing phase in DM patients, one of which involves using traditional medicine, specifically cherry plants. This study aimed to determine the effectiveness of cherry leaf extract cream in the healing process of incision wounds on day 3 on the skin of white rats induced by streptozotocin. The samples used were 12 white rats, aged approximately 3 months, with a body weight of 179.25 ± 12.64 g. The rats were injected intraperitoneally with streptozotocin at a dose of 45 mg/kg BW. After 72 hours, an incision was made in the paravertebral section with a length of 2 cm and a depth reaching the subcutaneous layer. Therapy was administered for 3 days in several groups. Group KI served as the negative control (cream base), KII as the positive control (metformin 4.5 mg/kg BW and silver sulfadiazine 0.1% cream), KIII (2 ml cherry leaf extract and topical therapy with 5% cherry leaf extract cream), and KIV (2 ml cherry leaf extract and 15% cherry leaf extract cream). The results of the average number of inflammatory cells in KI, KII, KIII, and KIV were 117.40±3.17; 87.87±8.97; 104.40±5.97; and 91.40±5.39, respectively, with P<0.05. The average number of new blood vessels in KI, KII, KIII, and KIV were 1.07±0.64; 4.87±0.64; 5.80±0.80; and 6.00±1.31, respectively, with P<0.05. The conclusion of this study is that the administration of cherry leaf extract and 15% cherry leaf extract cream can reduce inflammatory cell infiltration and accelerate the growth of new blood vessels

Keywords

Angiogenesis; cherry leaf extract; inflammation; wound healing; white rat

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References

Ajie, R.B. (2015). White dragon fruit (Hylocereus undatus) potential as diabetes mellitus treatment. Jurnal Majority, 4(1), 69-72.

Alouw, G.E.C., Fatimawali, & Lebang, J.S. (2022). Uji aktivitas antibakteri ekstrak etanol daun kersen (Muntingia calabura L.) terhadap bakteri Staphylococcus aureus dan Pseudomonas aeruginosa dengan metode difusi sumuran. Jurnal Farmasi Medica, 5(1), 36-44.

Anief, M. (2010). Ilmu Meracik Obat. Yogyakarta: Gadjah Mada University Press.

Anisa, N. & Najib, S.Z. (2022). Skrining fitokimia dan penetapan kadar total fenol flavonoid dan tanin pada ekstrak etanol daun kersen (Muntingia calabura L.). Indonesian Journal Pharmaceutical and Herbal Medicine, 1(2), 96-104.

Balqis, U., Masyitha, D., & Febrina, F. (2014). Proses penyembuhan luka bakar dengan gerusan daun kedondong (Spondias dulcis F.) dan vaselin pada tikus putih (Rattus norvegicus) secara histopatologis. Jurnal Medika Veterinaria, 8(1): 9-14.

David, A.V.A., Arulmoli, R., & Parasuraman, S. (2016). Overviews of biological importance of quercetin: A bioactive flavonoid. Pharmacognosy reviews, 10(20), 84-89.

Enoch, S. & Leaper, D.J. (2008). Basic science of wound healing. Surgery (Oxford), 26(2): 31-37.

Fish, R.E., Brown, M.J., Danneman, P.J., & Karas, A.Z. (2008). Anasthesia and Analgesia in Laboratory Animals, 2 Edition. Academic Press, AS.

Han, M.C., Durmus, A.S., Karabulut, E., & Yaman, I. (2005). Effects of turkish propolis and silver sulfadiazine on burn wound healing in rats. Revue de Medecine Veterinaire, 156(12), 624-727.

Hidayat, A.R. & Nurhayati, I. (2014). Perawatan kaki pada penderita diabetes militus di rumah. Jurnal Permata Indonesia, 5(2), 49-54.

Hidayati, H., Salim, M.N., Balqis, U., Masyitha, D., Iskandar, C.D., Darmawi, D., & Harris, A. (2019). Efficacy of Jatropha (Jatrophacurcas L.) sap cream in inflammation phase of wound healing on mice skin. Jurnal Medika Veterinaria, 13(2): 232-238.

Khotimah, S.N. & Muhtadi, A. (2016). Beberapa tumbuhan yang mengandung senyawa aktif antiinflamasi. Farmaka, 14(2), 28-40.

Kiernan, J.A. (1990). Histological and Histochemical Methods: Theory and Practice. Edisi 2. Oxford: Pergamon Press.

Kimura, Y., Sumiyoshi, M., Kawahira, K., & Sakanaka, M. (2006). Effects of ginseng saponins isolated from red ginseng roots on burn wound healing in mice. British Journal of Pharmacology, 148(6), 860-870.

Li, K., Diao, Y., Zhang, H., Wang, S., Zhang, Z., Yu, B., Huang, S., & Yang, H. (2011). Tannin extracts from immature fruits of Terminalia chebula Fructus Retz. promote cutaneous wound healing in rats. BMC Complementary and Alternative Medicine, 11(1): 1-9.

Luandayanti, D., Rifa’i, M., & Aulanni’am. (2013). Studi ekstrak metanol biji mahoni (Swietenia mahagoni) terhadap SOD serum tikus putih (Rattus norvegicus) pasca induksi MLD-STZ. Jurnal Biotropika, 1(6), 247-251.

Maigoda, T.C. (2022). Gel Ekstrak Daun Jambu Biji dan Daun Senduduk: Dampaknya terhadap Penanda Inflamasi, Bakteriologis, Diameter Luka, dan Kolagen pada Luka Kaki dengan Diabetes. Jawa Tengah: Penerbit NEM.

Mardiyantoro, F., Munika, K., Sutanti, V., Cahyati, M., & Pratiwi, A.R. (2018). Penyembuhan Luka Rongga Mulut. Malang: UB Press.

Nadira, L.A., Jayawardhita, A.A.G., & Adi, A.A.A.M. (2021). Pemberian salep ekstrak daun kersen, efektif meningkatkan proses angiogenesis pada kesembuhan luka insisi kulit mencit hiperglikemia. Indonesia Medicus Veterinus, 10(6), 851-860.

Ningrum, L.P., Salim, M.N., & Balqis, U. (2017). Pengaruh ekstrak daun jamblang (Syzygium cumini L.) terhadap histopatologi hepar tikus putih (Rattus norvegicus) diabetes mellitus. Jurnal Ilmiah Mahasiswa Veteriner, 1(4), 695-701.

Rosyidulibad, M., Nasution, T.H., & Andarini, S. (2013). Pengaruh ekstrak daun kersen (Muntingia calabura) terhadap derajat eritema pada proses inflamasi marmut (Cavia porcellus) dengan luka bakar derajat II dangkal. Jurnal Ilmu Keperawatan, 1(2), 157-161.

Salim, M.N., Masyitha, D., Harris, A., Balqis, U., Iskandar, C.D., Hambal, M., & Darmawi. (2018). Anti-inflammatory activity of Jatropha curcas Linn. latex in cream formulation on CD68 expression in mice skin wound. Veterinary World, 11(2), 99-103.

Salim, M.N., Silvia, M., Aliza, D., Masyitha, D., Iskandar, C.D., Rusli., Sugito., Hasan, A., Sabri, M., & Harris, A. (2020). Efficacy of Jatropha curcas latex cream in the epithelialization phase of wound healing in mice skin. E3S Web of Conferences, 151(1), 1-6.

Saputra, N.T., Suartha, I.N., & Dharmayudha, A.A.G.O. (2018). Agen diabetagonik streptozotocin untuk membuat tikus putih jantan diabetes mellitus. Buletin Veteriner Udayana, 10(2), 116-121.

Sutrisna, E.M. (2016). Herbal Medicine: Suatu Tinjauan Farmakologis. Surakarta: Muhammadiyah University Press.

Taher, M., Zakaria, T.M.F.S., Susanti, D., & Zakaria, Z.A. (2016). Hypoglycaemic activity of ethanolic extract of Garcinia mangostana Linn. in normoglycaemic and streptozotocin-induced diabetic rats. BMC Complementary and Alternative medicine, 16(1), 1-12.

Widayoko, A. (2021). Invensi Integritas Trensains. Deepublish Publisher, Yogyakarta.

Zhang, M., Lv, X.Y., Li, J., Xu, Z.G., & Chen, L. (2008). The characterization of high-fat diet and multiple low-dose streptozotocin induced type 2 diabetes rat model. Experimental Diabetes Research, 2008(1): 1-9.

DOI: https://doi.org/10.21157/j.med.vet..v18i2.40208