7. Effect of Soya Wastes Probiotic and Palm Kernel Addition Fermented by Aspergillus niger to the Cellulase Activity in Broilers Digestive Tract

Nurliana Nurliana, Anna Farida, Sugito Sugito, Al Azhar, Razali Razali, Cut Nila Thasmi

Abstract


This study aimed to determine the cellulase activity in broilers digestive tract after the treatment of soya wastes and palm kernel fermented by A.niger (AKBIS Prob) in the ration. This study used a complete randomized factorial design, which consists of two factors, namely the AKBIS Prob composition factor and the duration of AKBIS Prob. Twenty four broilers were divided into four groups by randomized and three replications of each. The feed supplement combinations were P0 (commercial), P1 (commercial and AKBIS Prob 2%), P2 (commercial and AKBIS Prob 4%), and P3 (commercial and AKBIS Prob 6%). The intestine preparation was taken at the 22 and 36 days to extraction and detection the cellulase activity concentration. Data were analyzed using variance analysis of factorial patterns. AKBIS Prob 2,4 and 6% was no effect (P> 0.05) to the cellulase activity in the small and large intestine, while the treatment duration was significant (P<0,05) in the small intestine but neither in the large intestine (P>0,05) to the cellulase activity. The addition of AKBIS Prob 2.4 and 6% had no effect (P> 0.05) on the concentration of cellulase activity in the small intestine and large intestine, while the duration the treatment was very significant (P <0.05) in the small intestine and neither in the large intestine (P> 0.05) on the concentration of cellulase activity. The AKBIS administration in the ration for 22 days can increase the activity and concentration of cellulase activity in the small intestine of the digestive tract of broiler chickens.

Keywords


broilers; fermentation; A.niger probiotic; enzyme activity; cellulase

Full Text:

PDF

References


Andhika, KS., Sukamto B. dan Dwiloka B. 2014. Efisiensi penggunaan protein pada ayam broiler dengan pemberian pakan mengandung tepung daun kayambang (Salvinia molesta). Agripet. 14(2) : 23-27.

Anon, D. 2004. Pengaruh penambahan probiotik Bacillus sp pada pakan komersil terhadap konversi pakan dan pertumbuhan ikan patin. Jurnal Akuakultur Indonesia. 3(1) :15-18.

Anonimous. 2010. Fermentasi(http:jajo66.files.wordpress.com/2008/03/06 fermentasi.pdf). (10 Mei 2016).

Arnata, I W. 2009. Teknologi Bioproses Pembuatan Bioetanol dari Ubi Kayu Menggunakan Trichoderma viride, Aspergillus niger dan Saccharomyces cerevisiae. Tesis. Institut Pertanian Bogor. Bogor.

Astutik, R.P., Kuswytasari, N.D., Shovitri, M. 2011.Uji Aktivitas Enzim Selulase dan Xilanase Isolat Kapang Tanah Wonorejo Surabaya. Laporan

Penelitian. Institut Teknologi Sepuluh Nopember Surabaya. Surabaya.

Bouliane, M. 2003. Can We Farm Poultry Without Antimicrobials.Faculty of Veterinary Medicine, University of Montreal.

Chasanah E., Dini IR. dan Nisa RM. 2013. Karakterisasi enzim selulase PMP 0126Y dari limbah pengolahan agar. JPB Perikanan. 8(2) : 103–114.

Fardiaz S. 1989. Mikrobiologi Pangan. Pusat Antar Universitas. Institut Pertanian Bogor. Bogor.

Fitasari, E. 2009.Pengaruh penggunaan probiotik dan enzim papain dalam pakan terhadap karakteristik dan mikroflora usus, serta penampilan produksi ayam pedaging. Tesis. Program Pascasarjana Universitas Brawijaya. Malang.

Harimurti, S., E.S.Rahayu, Nasroedin dan Kurniasih. 2014. Bakteri asamlaktat dari intestin ayam sebagai agensia probiotik. Animal Production. 9 (2) : 82-91.

Ichwan, W.M.W. 2005.Membuat Pakan Ayam Ras Pedaging. Cetakan Kedua. Agromedia Pustaka. Jakarta.

Inggrid, M dan Suharto. 2012. Fermentasi Glukosa oleh Aspergillus nigerMenjadi Asam Glukonat. Laporan Penelitian. Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Katolik Parahayangan. Bandung.

Mabelebele, M., Alabi, O.J., Ngambi, J.W., Norris, D. and Ginindza, M.M. 2013. Comparison of gastrointestinal tract and pH value od digestive organs of ross 308 broiler and indigenous venda chicken feed the same diet. Asian Journal of Animal and Veterinary Advance. 1(7):1-6.

Masfufatun. 2013. Isolasi dan karakterisasi enzim selulase. JurnalBiologi.15(2):29-33.

Matin, M., Herlinasari dan Hermiati. 2013. Pengaruh penggunaan probiotik dalam ransum terhadap performans produksi susu peternakan sapi perah rakyat. Jurnal Ilmu Ternak.17(2):42-47.

Mirzaie, S., Zaghari, M., Aminzadeh, S and Shivazad, M. 2012. The effect of non starch polysaccharides content of wheat and xylanase supplementation on the intestinal amylase, aminopeptidase and lipase activities, ileal viscosity and fat digestibility in layer diet. Iranian Journal of Biotechnology.10(3): 208-214.

Mitchell dan Lemme. 2008. Treatment of commercial

broiler chickens with a characterized culture of caecal bacteria to reduce salmonella colonization. Poultry Sci. 74:1093-1101.

Nurliana, Sitti W. dan D. Masyitha. 2015. Pemberian ampas kedelai dan serat buah sawit yang difermentasi Aspergillus nigerdalamuakan Untuk peningkatan produktivitas ayam petelur. Laporan Penelitian . Universitas Syiah Kuala. Banda Aceh.

Puspa, R.W., W. Busono dan R. Indrati. 2011. Pengaruh suhu kandang yang berbeda terhadap performans ayam pedaging periode starter. Jurnal Ilmu Ternak. 12(5) : 125-128.

Ramadhan, N. 2012.Isolasi dan Seleksi Jamur Selulolitk dari Tanah Gambut di Perkebunan Karet Desa Rimbo Panjang Kabupaten Kampar Riau.Disertasi. Universitas Riau. Pekanbaru.

Rasyaf, M. 1994. Bahan Makanan Unggas di Indonesia. Catakan Ketiga. Kanisius.Yogyakarta.

Sulistyawan, I.H. 2015.Perbaikan kualitas pakan ayam broiler melalui fermentasi dua tahap menggunakan Trichoderma reseei dan Saccaromyces cerevisiae.Agripet.15( 1) : 66-71.

Supriyadi, T. P., H. Hamid dan A. Sinurat. 1998. Fermentasi ampas inti sawit secara substrat padat dengan menggunakan Aspergillus niger. Jurnal Ilmu Ternak dan Veteriner. 3 (3) :165-170.

Teather, R.M. and P.J.Wood. 1981. Use of congo redpolysaccharide interactions in enumeration and characterization of cellulolytic bacteria fromthe bovine rumen. Applied and Environmental Microbiology.4:777-780.

Wahyuningtyas, P., B.D.Argo dan W.A Nugroho. 2013. Studi pembuatan enzim selulase dari mikrofungi Trichoderma reeseidengan substrat jerami padi sebagai katalis hidrolisis enzimatik pada produksi bioetanol. Jurnal Bioproses Komoditas Tropis. 1(1):23-28.

Wido, E.Gandara dan I.Mokoginta. 2015. Pengaruh penambahan probiotik Bacillus sp. pada pakan komersil terhadap konversi pakan dan pertumbuhan ikan patin. Jurnal Penelitian Perikanan Indonesia. 6(2):62-67.

Zhang, Y.H.P, M.E.Himmel dan J.R.Mielenz. 2006. Outlook for cellulase improvement: screening and selection strategies. Biotechnol Adv. 24: 452–481.




DOI: https://doi.org/10.21157/j.med.vet..v13i1.4207

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 by author and J. Med. Vet.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Creative Commons License
J. Med. Vet. is licensed under a Creative Commons Attribution 4.0 International License.