Effect of Ethyl Acetate Extract of Malacca Leaf (Phyllanthus emblica) on the Growth of Plasmodium falciparum in Vitro

Habibah Annum Sir; Nuzul Asmilia; T. Armansyah TR

doi:10.21157/j.med.vet..v18i2.42732

Effect of Ethyl Acetate Extract of Malacca Leaf (Phyllanthus emblica) on the Growth of Plasmodium falciparum in Vitro

Habibah Annum Sir, Nuzul Asmilia, T. Armansyah TR

Abstract

This study aimed to determine the antiplasmodium activity of ethyl acetate extract of malacca leaf (Phyllanthus emblica L.) on Plasmodium falciparum in vitro. In this study, five treatment groups with extract dosage of 100, 75, 50, 25, and 5 μg / ml were used. For positive control, antimalarial drug artesdiaquine was given. Plasmodium culture used the candle-jar method and the antiplasmodium activity test was carried out by microculture method in vitro. This research used a complete randomized design (RAL), data was analyzed using analysis of variance (ANOVA) and continued with Duncan's tests. The inhibition concentration (IC₅₀) of malacca leaf extract was analyzed using PROBIT SPSS. Antiplasmodium activity was expressed by IC₅₀ value which is the ability to inhibit 50% of Plasmodium growth. The results showed that the IC50 value of the extract was 17,849 μg/ml. It can be concluded that the ethyl acetate extract of malacca leaf had good activity as antiplasmodium and the best concentration was 25 μg / ml.

Keywords

Phyllanthus emblica L.; Plasmodium falciparum; antiplasmodium; malaria

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References

Akbar, H.R. 2010. Isolation and Identification of Flavonoid Groups of Dandang Gendis Leaves (Clinacanthus nutans) Potentially As Antioxidants. Thesis. Bogor: Faculty of Mathematics and Natural Sciences. Bogor Agricultural Institute.

Alkandahri, M. Y., Berbudi, A., & Subarnas, A. (2018). Active compounds and antimalaria properties of some medicinal plants in Indonesia–a review. Systematic Reviews in Pharmacy, 9(1), 64-69.

Arora, A., Ceccagnoli, M., & Cohen, W. M. (2008). R&D and the patent premium. International journal of industrial organization, 26(5), 1153-1179.

Arsin, A.A. 2012. Malaria in Indonesia. Epidemiological Review and Aspects.

Barcus, M. J., Basri, H., Picarima, H., Manyakori, C., Elyazar, I., Bangs, M. J., ... & Baird, J. K. (2007). Demographic risk factors for severe and fatal vivax and falciparum malaria among hospital admissions in northeastern Indonesian Papua.

Bhandari, P. R., & Kamdod, M. A. (2012). Emblica officinalis (Amla): A review of potential therapeutic applications. International Journal of Green Pharmacy (IJGP), 6(4).

Dubar, F., Egan, T. J., Pradines, B., Kuter, D., Ncokazi, K. K., Forge, D., ... & Biot, C. (2011). The antimalarial ferroquine: role of the metal and intramolecular hydrogen bond in activity and resistance. ACS chemical biology, 6(3), 275-287.

Gaspersz, V. (1991). Experimental design method. Bandung, Indonesia, CV. Armico.

Gessler, M. C., Nkunya, M. H. H., Mwasumbi, L. B., Heinrich, M., & Tanner, M. (1994). Screening Tanzanian medicinal plants for antimalarial activity. Acta tropica, 56(1), 65-77.

Hilou, A., Nacoulma, O. G., & Guiguemde, T. R. (2006). In vivo antimalarial activities of extracts from Amaranthus spinosus L. and Boerhaavia erecta L. in mice. Journal of ethnopharmacology, 103(2), 236-240.

Isaac. 2005. Bibliometric Analysis of Malaria Disease Research Articles in Indonesia 1970-April 2004 Using PubMed Online Database. Journal of Library and Information Studies. 1(2): 17-25.

Juliana, A., Vina, S. Aisyah, and I. Mustapha. 2010. Isolation of characterization of terpenoid-derived compounds from the n-hexane Fraction momordica charantia L. Journal of Science and Technology. Kim. 1: 88-93.

Kamdod, M. A. 2012. Emblica officinalis (Amla): A review of potential applications. International Journal of Green Pharmacy 6(4): 257-269. 12 March 2015.

Kayser, O., Kiderlen, A. F., & Croft, S. L. (2002). Natural products as potential antiparasitic drugs. Studies in natural products chemistry, 26, 779-848. Masagena Press: Makassar.

Minister of Health of the Republic of Indonesia. (2011). Health Data and Information Window Bulletin. Malaria Epidemiology in Indonesia. First Quarter. Jakarta: Ministry of Health of the Republic of Indonesia.

Nguyen-Pouplin, J., Tran, H., Tran, H., Phan, T. A., Dolecek, C., Farrar, J., ... & Grellier, P. (2007). Antimalarial and cytotoxic activities of ethnopharmacologically selected medicinal plants from South Vietnam. Journal of ethnopharmacology, 109(3), 417-427.

Novita, L. (2009). Malaria Diagnosis and Management. Doctor's Files. Faculty of Medicine. Riau University. http://www.Doctors-Filez.tk. December 2, 2014.

Olliaro, P. L., & Bloland, P. B. (2001). Clinical and public health implications of antimalarial drug resistance. In Antimalarial chemotherapy: mechanisms of action, resistance, and new directions in drug discovery (pp. 65-83). Totowa, NJ: Humana Press.

Panggabean, W. (2010). Characteristics of malaria sufferers in Dumai City in 2005-2009. Thesis. Faculty of Public Health. University of North Sumatra.

Pinmai, K., Hiriote, W., Soonthornchareonnon, N., Jongsakul, K., Sireeratawong, S., & Tor-Udom, S. (2011). In vitro and in vivo antiplasmodial activity and cytotoxicity of water extracts of Phyllanthus emblica, Terminalia chebula, and Terminalia bellerica. Journal of the Medical Association of Thailand, 93(12), 120.

Ramadhani, D. and Nurhayati, S. (2012). Automation of the calculation of the percentage of parasitemia Plasmodium falciparum using software Nish Element D 2.3. Computational Workshop in Nuclear Science and Technology, 173-186.

Ramdja, M. (1997). Mechanism of Resistance of Plasmodium falciparum To Chloroquine. MEDIKA, 23(11): 873.

Ravikumar, S., Inbaneson, S. J., & Suganthi, P. (2012). In vitro antiplasmodial activity of chosen terrestrial medicinal plants against Plasmodium falciparum. Asian Pacific Journal of Tropical Biomedicine, 2(1), S252-S256

Rompas, R. A., Edy, H. J., & Yudistira, A. (2012). Isolasi dan identifikasi flavonoid dalam daun lamun (Syringodium isoetifolium). Pharmacon, 1(2).

Saxena, S., Pant, N., Jain, D. C., & Bhakuni, R. S. (2003). Antimalarial agents from plant sources. Current science, 1314-1329.

Sio, S. W., Sun, W., Kumar, S., Bin, W. Z., Tan, S. S., Ong, S. H., ... & Tan, K. S. (2007). MalariaCount: an image analysis-based program for the accurate determination of parasitemia. Journal of microbiological methods, 68(1), 11-18.

DOI: https://doi.org/10.21157/j.med.vet..v18i2.42732