Scabies is an infectious disease which is caused by Sarcoptes scabiei mites and considered as an emerging/re-emerging parasitic disease. Recent study using the Polymerase Chain Reaction (PCR) method has a higher sensitivity for detecting mites than using the skin scraping method. This study aims to detect the Second Internal Transcribed Spacer (ITS-2) encoding gene of S. scabiei. ITS-2 is a highly conserved area and always used to identify and differentiate host species infected by S. scabiei. S. scabiei isolates were obtained from skin scrapings from stray cat suspected of being infected with Scabies from Pasar Induk Bondowoso, Pasar Tamanan and Pasar Pujer. Detection on the presence of S. scabiei was carried out microscopically and then confirmed by molecular examination using the PCR method and visualized by electrophoresis on 2% agarose gel. The Polymerase Chain Reaction (PCR) results showed DNA bands at 304 bp Based on the result it can be concluded that samples are positively infected by Scabies can be detected by the ITS-2 encoding gene of Sarcoptes scabiei.

Aprilianti, Y., Rahmiati, D.U., Setyowati, E.Y., Dahlan, A. (2020). Potensi Anastetik Sediaan jadi Kombinasi Ketamin Hidroklorida, Atropin Sulfat dan Xylazin Hidroklorida pada Kucing Jantan Lokal. Indonesia Medicus Veterinus,9(3): 475-487.

Desiandura, K., Lastuti, N.D.R., Suwanti, L.T., Handijatno, D. (2017). Molecular identification of Sarcoptes scabiei var. cuniculi from Surabaya and Malang regions of East Java. Indonesian Journal of Tropical and Infectious Disease, 6(6): 150-153.

Fraser, T. A., Martin, A., Polkinghorne, A., Carver, S. (2018). Comparative diagnostic reveals PCR assays on skin scrapings is the most reliable method to detect Sarcoptes scabiei infestations. Vet Parasitol,251: 119-124.

Hengge, U. R., Currie, B. J., Jager, G., Lupi, O., Schwartz, R. A. (2006). Scabies: a Ubiquitous Negleted Skin Disease. The Lancet Infectious Disease, 6(12): 769-779.

Hermawan, I. P., Sari, D. A. K., Rahman, M. N. (2021). Deteksi Parasit Darah pada Kucing Liar (Stray Cats) dengan Metode Pewarnaan MDT di Pasar Tradisional Surabaya. Jurnal Kajian Veteriner, 9(3): 142-147.

Lastuti, N. D. R., Rohman, A., Handiyatno, D., Chrismanto, D., Desiandura K. (2019). Sequence analysis of the Cytochrome C oxidase subunit 1 Gene of Sarcoptes scabiei isolate from goats and rabbits in East Java, Indonesia. Vet World, 12(7): 959-964.

Ljunggren, E. L. (2005). Molecular analysis of Sarcoptes scabiei. Faculty of Veterinary Medicine and Animal Science, Uppsala.

Loeffelholz, M., & Deng, H. (2006). PCR and its variations. Advanced Techniques in Diagnostic Microbiology (pp.166-183Springer US.

Makouloutou, P., Suzuki, K., Yokoyama, M., Takeuchi, M., Yanagida, T., Sato, H. (2015). Involvement of two genetic lineages of Sarcoptes scabiei mites in a local mange epizootic of wild mammals in Japan. J Wildl Dis, 51(1): 69-78

Niedringhaus, K. D., Brown, J. D., Sweely, K. M., Yabsley, M. J. (2019). A Review of Sarcoptic Mange in North American Wildlife. The International Journal for Parasitology: Parasites and Wildlife. 9: 285-297.

Ningrum, N. M. A. A., Soma, I. G., Batan, I. W. (2023). Treatment of Scabiosis in Domestic Cat with Combination Therapy of Ivermectin and Sulphur Soap : A Case Report. Indonesia Medicus Veterinus,12(5): 657-667.

Peltier, S. K., Brown, J. D., Ternent, M., Niedringhaus, K.D., Schuler, K., Bunting, E. M. (2017). Genetic characterization of Sarcoptes scabiei from black bears (Ursus americanus) and other hosts in the Eastern United States. J Parasitol,103(5): 593-597.

Siagian, T. B. (2022). Infestasi Ektoparasit pada Kucing Liar di Kampus IPB Gunung Gede. Jurnal Sains Terapan, 12(2): 15-25.

Tarigan, S. (2007). Vaksin skabies dibutuhkan namun sulit diwujudkan. Balai Besar Penelitian Veteriner. Wartazoa,17(1): 38-45.

Yudaniayanti, I.S., Maulana, E., Ma’ruf, A. (2010). Profil penggunaan kombinasi Ketamin-Xylazine dan Ketamin-Midazolane sebagai anastesi umum terhadap gambaran fisiologis tubuh pada kelinci jantan. Veterinaria Medika,3(1): 23-30