Pengaruh Polimorfisme Gen Heat Shock Protein 70 (HSP70) SacII terhadap Toleransi Panas Itik Lokal Sumatera Barat
Abstract
ABSTRAK. Tujuan dari penelitian ini adalah untuk mengidentifikasi pengaruh polimorfisme gen heat shock protein 70 (HSP70) terhadap sifat toleransi panas. Genotiping dilakukan pada 110 itik lokal dari 4 populasi itik Sumatera Barat (pitalah, bayang, kamang, dan payakumbuh). Enzim restriksi yang digunakan adalah SacII. Cekaman panas dilakukan selama 1 jam dengan sampel itik lokal sebanyak 24 ekor untuk menganalisa status hematologi. PCR-RFLP dan Sanger sequencing assay digunakan untuk mengidentifikasi polimorfisme. Analisis polimorfisme dilakukan dengan menggunakan Software MEGA 7 dan pengaruhnya dianalisis dengan ANOVA menggunakan software SAS 9.4. Produk amplifikasi yang dihasilkan yaitu 466 pasang basa. Lokus HSP70/SacII mengenali satu situs SNP (Single nucleotide Polymorphism) g.1702T>C, menghasilkan dua alel (T dan C) dan tiga genotype (TT, TC, CC). Gen HSP70/SacII bersifat polimorfik pada semua populasi. Hasil penelitian menunjukkan bahwa alel T memiliki frekuensi yang lebih tinggi daripada alel C pada semua populasi. Analisis chi-kuadrat (χ2) menunjukkan bahwa semua populasi itik lokal memenuhi kaidah kesetimbangan Hardy-Weinberg. Pengaruh genotipe gen HSP70 terhadap status hematologi menunjukkan perbedaan (P<0,05) terhadap nilai heterofil dan nilai H/L dengan genotipe CT menunjukkan toleransi panas yang lebih baik dibanding genotipe lainnya. Gen HSP70/SacII dapat dijadikan marka molekuler sifat toleransi panas pada itik lokal berdasarkan keragaman dan pengaruhnya terhadap status hematologi.
(The effect of heat shock protein 70 (HSP70) SacII gene polymorphism on heat tolerance of West Sumatera local duck)
ABSTRACT. The objective of this study was to identify the effect of HSP70/SacII gene polymorphism associated with the thermotolerance trait. Genotyping was performed on 110 local duck from the West Sumatera ducks population (pitalah, bayang, kamang, and payakumbuh). The restriction enzyme used was SacII. Heat stress was done for 1 hour with samples used as much as 24 birds to analyze hematological status. PCR-RFLP and Sanger sequencing assays were used to identify polymorphism. Analysis of polymorphism was conduct by MEGA 7 software and its effect is analyzed with ANOVA using Statistical SAS 9.4 software. The product of amplification was 466 bp. HSP70/SacII recognized one SNP g.1702T>C, producing two alleles (T and C) and three genotypes (TT,TC,CC). The HSP70/SacII locus were polymorphic in all population. T allele had a higher frequency than C allele in all populations. The analysis of chi-square (χ2) showed that all local ducks population were fit with Hardy-Weinberg equilibrium. The effect of HSP70/SacII gene on hematological status showed differences (P<0,05) of heterophil and H/L ratio with CT genotype show better heat tolerance than other genotypes. HSP70/SacII gene can be used as a marker of heat tolerance in local ducks based on polymorphism and its effect on hematological status.
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Ali, M.S., Yang, H.S., Jeong, J.Y., Moon, S.H., Hwang, Y.H., Park, G.B., Joo, S.T., 2008. Effect of chilling temperature of carcass on breast meat quality of duck. Poult. Sci. 87: 1860-1867.
Blecha, F., 2000. Immune system response to stress. In: Moberg, GP, Mench JA (eds). The Biology of Animal Stress: Basic Principle and Implications for Animal Welfare. London (UK): CABI Publishing. pp: 111-146.
Campo, J.L., Gil M.G., Munoz, I., Alonso, M., 2000. Relationship between bilateral asymmetry and tonic immobility reaction or heterofil to limfosit ratio in five breeds of chickens. Poult. Sci. 79: 453-459.
Cetin, E., Guclu B.K., Cetin, N., 2011. Effect of dietary humate and organic acid supplementation on social stress induced by high stocking density in laying hens. J. Animal and Vet. Adv. 10(18): 2402-2407.
Davis, A.K., Maney, D.L., Maerz, J.C., 2008. The use of leukocyte profiles to measure stress in vertebrates: a review for ecologists. Func. Ecol. 22:760-772.
Emadi, M., Kermanshahi, H., Maraoufyan, E., 2007. Effect of turmenic rhizome powder on the activity of some blood enzyme in broiler chicken. Int. J. Poulty. Sci. 6(1): 48-51.
Gong, W.J., Golic, K.G., 2006. Loss of HSP70 in drosophila is pleiotropic, with effects on thermotolerance, recovery from heat shock and neuro degeneration. Genetics: 172: 275-286.
Gudev, D., Popova-Ralcheva, S., Ianchev, I., Moneva, P., 2011. Effect of betaine and air ammonia concentration on broiler performance, plasma corticosterone level, lymphoid organ weights and some haematological indices. Biotech. in Animal Husb. 27(3): 687-70.
Lindquist, S., Craig, E.A., 1988. The heat-shock proteins. Annual Review of Genetics. 22: 631-677.
Mazzi, C.M., Ferro, J.A., Ferro, M.I.T., Savino, V.J.M., Coelho, A.A.D., Macari, M., 2003. Polymorphism analysis of the HSP70 stress gene in broiler chickens (Gallus gallus domesticus) of different breeds. Genetics and Molecular Biology. 26: 275-281.
NCBI, 2018. Primer designing tools. http://www.ncbi.nlm.nih.gov/tools/primer-blast/. [14 Januari 2018].
Nei, M., and Kumar, S., 2000. Molecular Evolution and Phylogenetics. Oxford Univ Pr. New York.
Noor, R.R., 2010. Genetika Ternak. Penebar Swadaya. Jakarta.
Noor, R.R., dan Seminar K.B., 2009. Rahasia dan Hikmah Pewarisan Sifat (Ilmu Genetika dalam Al-Qur’an). Bogor. Penerbit IPB Press. Hal:109.
O’Driscoll, K.K.M., Broom, D.M., 2011. Does access to open water affect the behavior of Peking Ducks (Anas platyrynchos). Poult. Sci. 90: 229-307.
Ristossa, F., 1962. A new puffing pattern induced by heat shock and DNP in drosophila. Experientia. 18: 571-573.
Sambrook, J., and Russell, D., 2001. Molecular Cloning: A Laboratory Manual. 3rd Ed. Cold Spring Harbor Laboratory Press. USA.
Steel, R.G.D., dan Torrie, J.H., 1993. Prinsip dan Prosedur Statistika. Suatu Pendekatan Biometrik. Edisi kedua. Gramedia Pustaka Utama. Jakarta.
Tambasco, D.D., Pas, C.C.P., Tambasco, S.M., Pereira, A.P., Alencar, M.M., Freitas, A.R., Coutinho, L.L., Packer, I.U., Regitano, L.C.A., 2003. Candidate genes for growth traits in beef cattle cross Bos Taurus × Bos Indicus. J. An. Breed. Genet. 120: 51-56.
Tamzil, M.H., Noor, R.R., Hardjosworo, P.S., Manalu, W., Sumantri, C., 2013. Keragaman gen heat shock protein 70 ayam Kampung, ayam Arab dan ayam Ras. J. Vet. 14:317-326.
Vasconcellos, L.P.M.K., Talhari, D.T., Pereira, A.P., Coutinho, L.L., Regitano, L.C.A., 2003. Genetic characterization of aberdeen angus cattle using molecular markers. Genetic. Mol. Biol. 26: 133-137.
Weir, B.S., 1996. Genetic Data Analysis II : Method for Discrete Population Genetic Data. 2nd Ed. Sinauer Associates. Sunderland.
Xia, M., Gan, J., Luo, Q., Zhang, X., Yang, G., 2013. Identification of duck HSP70 gene, polymorphism analysis and tissue expression under control and heat stress conditions. British Poult. Sci: 54(5): 562-566.
Zhen, F.S., Du, H.L., Xu, H.P., Luo, Q.B., Zhang, X.Q., 2006. Tissue and allelic-specific expression of HSP70 gene in chickens: basal and heat-stress-induced mRNA level quantified with real-time reverse transcriptase polymerase chain reaction. British Poult. Sci. 47: 449-455.
DOI: https://doi.org/10.17969/agripet.v19i2.14876
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