Polimorfisme Protein Darah Itik Pegagan dengan Metode PAGE

Meisji Liana Sari, Ronny Rachman Noor, Peni S. Hardjosworo, Chairun Nisa

Abstract


polymorphism of blood protein pegagan duck by PAGE method

ABSTRACT. Pegagan duck is one of local duck species which comes from Ogan Ilir (OI) Regency, South Sumatera. This species is not popular, yet. But, it has spread out along Ogan river which comprises of three districts e.g Tanjung Raja, Inderalaya and Pemulutan. The ecosystem in this three districts is dominated by swampy area. As far, there is relatively limited information of Pegagan duck compared to another local duck. So, for the first step was done a reseach to identify genetic characteristic from blood protein analyses by electrophoresis method. It is expected that this information will become the bases in determining of the policy of Pegagan duck development.
Polymorphism analyses of blood protein was done with 9 duck samples which taken randomly from 400 pegagan ducks. Protein locus observed were albumin (Alb), post albumin (Pa), transferrine (Tf), post transferrine-1 (Ptf-1), and post transferrine-2 (Ptf-2). Polymorphism analyses of blood protein of pegagan duck was characterized by allele A in locus Transferrine (TfA), Post transferrine-1 (Ptf-1A) and Post transferrine-2 (Ptf-2A) . Genetic diversity based on blood protein of Pegagan duck showed that gen frequency value in a range of 0,11 – 0,94 and high heterozygosis value that was 0,62 %.


Keywords


Pegagan duck, blood protein polymorphism, PAGE

Full Text:

PDF

References


Avise, J.C. 1994. Molecular Markers, Natural History and Evolution. Chapman and Hall, Inc., New York.

Azmi, Gunawan, dan S Edward. 2006. Karakteristik Morfologis dan Genetik Itik Talang Benih di Bengkulu. Seminar Nasional Peternakan dan Veteriner. Balai Penelitian Ternak. Pusat Penelitian dan Pengembangan Peternakan. Badan Penelitian dan Pengembangan Pertanian. Bogor. Hal 716-721.

Gahne B, Juneja RK, Gromlus J. 1977. Horizontal polyacrilamide gradient gel electrophoresis for the silmotanous phenotyping of transferin, post transferin, albumin and post albumin in blood plasma of cattle. J Anim Blood Biochem genet 7:59-64

Gunawan B et al. 1994. Korelasi Fenotipik dan Genetik Beberapa Sifat Produksi Telur Itik Alabio, Khaki Campbell, Tegal dan Persilangannya. Prosiding Pengolahan dan Komunikasi Hasil-Hasil penelitian Unggas dan Aneka Ternak. Ciawi, 20-22 Februari 1994. Balai Penelitian Ternak. Pusat Penelitian dan Pengembangan Peternakan. Badan Penelitian dan Pengembangan Pertanian. Bogor. Hal 49-54.

Harris M. 1994. Dasar-Dasar Genetika Manusia. Edisi ke 3. Jogjakarta. Gadjah Mada University Press.

Lestari. 2002. Pengkajian polimorfisme protein plasma darah ayam kampong dan ayam ras menggunakan analisis poliacrrilame gel electrophoresis (PAGE). J. Anim Sci and Tech 1(1): 18-25.

Marson, E.P., J.B.S. Ferraz, F.V. Meirelles, J.C.D.C. Balieiro, J.P.Eler, L.G.G.Figueiredo,& G.B. Mourau. 2005. Genetic characterization of European Zebu composite bivine using RFLP markers. Genet.Mol.Res.4:496-505.

Martojo H. 1992. Peningkatan Mutu Genetik Ternak. Departemen Pendidikan dan Kebudayaan. Direktorat Jendral Pendidikan Tinggi. Pusat Antar Universitas dan Bioteknologi Institut Pertanian Bogor.

Moiloli B. Napalitano F, Cattilo G. 2004. Genetic diversity between Piedmontase Marremana and Podolica cattle breeds. J. Hered 95:250-265.

Nei M. 1987. Molecular Evolutionary Genetics. Columbia University Press. New York.

Noor RR. 2008. Genetika Ternak. Jakarta. Penerbit PT. Penebar Swadaya.

Pramudyati YS. 2003. Pengkajian Teknologi Pemeliharaan Itik di Sumatera Selatan. Loka Pengkajian Teknologi Pertanian (LPTP) Puntikayu Sumatera Selatan.

Suryana. 2011. Karakterisasi Fenotipik dan Genetik Itik Alabio (Anas platyrhynchos Borneo) di Kalimantan Selatan Dalam Rangka Pemanfaatan dan Pelestarian Secara Berkelanjutan. Disertasi. Sekolah Pascasarjana, Institut Pertanian Bogor.

Wahyuni, A. Kajian Karakteristik Biologis Itik Cihateup dari Kabupaten Tasikmalaya dan Garut. Tesis. Pascasarjana Institut Pertanian Bogor.

Weir BS. 1996. Genetic Data Analysis: method for Discrete Population Genetic Data. Second ed. Sinauer Associates. Sunderland, MA USA.

Winaya A. 2010. Variasi Genetik Dan Hubungan Filogenik Populasi Sapi Lokal Di Indonesia Berdasarkan Molekuler DNA Mikrosatelit Kromosom Y dan Gen Cytochrome B. Disertasi. Bogor. Sekolah pascasarjana, Institut Pertanian Bogor.




DOI: https://doi.org/10.17969/agripet.v11i2.376

Article Metrics

Abstract view : 0 times
PDF - 0 times

Refbacks

  • There are currently no refbacks.


Copyright (c)




Creative Commons LicenseISSN: 1411-4623E-ISSN: 2460-4534
Copyright© 2000-2024 | ISSN: 1411-4623 | EISSN: 2460-4534 
Jurnal Agripet is licensed under a Creative Commons Attribution 4.0 International License.

 

Published by: 
Animal Husbandry DepartmentThe Faculty of Agriculture, Universitas Syiah Kuala 
associated with Animal Scientist's Society of Indonesia (HILPI)
Jl. Tgk. Hasan Krueng Kalee No. 3, Kopelma Darussalam,
Banda Aceh, 23111, Indonesia.
Phone: +62-81383736633
Email: jurnalagripet@usk.ac.id


Online Submissions & Guidelines Editorial Policies | Contact Statistics Indexing | Citations