Addition of Sodium Hyaluronate to Ringer Lactate-Egg Yolk Diluent on the Quality of Native Chicken Sperm

Khaeruddin Khaeruddin, Abdul Hakim Fattah, Bahri Syamsuryadi, Hermawansyah Hermawansyah

Abstract


This study aims to evaluate the effect of adding varying concentrations of sodium hyaluronate (SH) to a Ringer lactate-egg yolk diluent on the quality of native chicken spermatozoa during 48 hours of storage. A completely randomized design (CRD) was used with four treatment groups: control (diluent without SH), diluent with 63 M SH, 103 M SH, and 143 M SH. The study involved six replications with pooled semen from three native chickens aged approximately 10 months. Semen was collected, diluted, and stored at 5C for 48 hours. Parameters observed after storage included motility, viability, plasma membrane integrity, acrosome integrity, DNA damage, and mitochondrial activity. Data were analyzed using analysis of variance (ANOVA). The results indicated that the treatments had no significant effect (P0.05) on any of the spermatozoa quality parameters. The observed values for motility, viability, plasma membrane integrity, acrosome integrity, DNA damage, and mitochondrial activity were 46.67-50%, 95.11-96.55%, 92.77-95.55%, 4.77-5.44%, and 71.05-75.37%, respectively. In conclusion, the addition of sodium hyaluronate at concentrations of 63-143 M in a Ringer lactate-egg yolk diluent does not significantly affect the quality of native chicken spermatozoa during 48 hours of storage at 5C.

Keywords


Sodium hyaluronate; Pengencer; Penyimpanan; Spermatozoa Ayam

Full Text:

PDF

References


Agarwal, A., Barbăroșie, C., Ambar, R., Finelli, R., 2020. The impact of single-and double-strand DNA breaks in human spermatozoa on assisted reproduction. Int. J. Mol. Sci. 21, 3882.

Agarwal, A., Gupta, S., Sharma, R., 2016. Eosin-nigrosin staining procedure. In: Agarwal, A., Gupta, S., Sharma, R. (Eds.), Andrological Evaluation of Male Infertility: A Laboratory Guide. Switzerland: Springer, pp. 73–77.

Balogun, A.S., 2021. Optimizing liquid storage duration of two poultry species semen with plant based extender: semen preservation in poultry. Lett. Anim. Biol. 1, 1–2.

Barbarestani, S.Y., Samadi, F., Zaghari, M., Khademian, S., Pirsaraei, Z.A., Kastelic, J.P., 2024. A review of antioxidant strategies to improve reproduction in aging male broiler breeders. GeroScience 2024, 1–17.

Bebas, W., Pemayun, T.G.O., Damriyasa, I.M., Manntik-Astawa, I.N.,

Lactose-astaxanthin increases green jungle fowl’s sperm motility and reduces sperm DNA fragmentation during 5 o Celcius storage. Bali Med. J. 3, 152–156.

Budi, J.A., Bebas, W., Laksmi, D.N.D.I., 2020. Daya simpan semen ayam Cemani dalam pengencer susu skim fosfat pada suhu 4 ℃ berdasarkan motilitas dan daya hidup spermatozoa. Indones. Med. Veterinus 9, 705–715.

Costa, J., Braga, P.C., Rebelo, I., Oliveira, P.F., Alves, M.G., 2023. Mitochondria quality control and male fertility. Biology (Basel). 12, 827.

Durairajanayagam, D., Singh, D., Agarwal, A., Henkel, R., 2021. Causes and consequences of sperm mitochondrial dysfunction. Andrologia 53, e13666.

Eslami, M., Ghaniei, A., Rad, H.M., 2016. Effect of the rooster semen enrichment with oleic acid on the quality of semen during chilled storage. Poult. Sci. 95, 1418–1424.

Fattah, A., Sharafi, M., Masoudi, R., Shahverdi, A., Esmaeili, V., 2017. L-Carnitine is a survival factor for chilled storage of rooster semen for a long time. Cryobiology 74, 13–18.

Galvez-Martin, P., Soto-Fernandez, C., Romero-Rueda, J., Cabañas, J., Torrent, A., Castells, G., Martinez-Puig, D., 2023. A novel hyaluronic acid matrix ingredient with regenerative, anti-aging and antioxidant capacity. Int. J. Mol. Sci. 24, 4774.

Gibb, Z., Lambourne, S.R., Quadrelli, J., Smith, N.D., Aitken, R.J., 2015. L-Carnitine and pyruvate are prosurvival factors during the storage of stallion spermatozoa at room temperature. Biol. Reprod. 93, 104.

Gosálvez, J., Fernández, C.L., Johnston, S.D., Bartolomé-Nebreda, J., 2024. Role of DNase activity in human sperm DNA fragmentation. Biomolecules 14, 304.

Jeyendran, R., Lazarevic, A., Fishel, L., Ivanovic, M., Levrant, S., 2021. ATP as indicator of sperm normalcy and reproductive fitness. Fertil. Steril. 116, e351.

Kameni, S.L., Meutchieye, F., Ngoula, F., 2021. Liquid storage of ram semen: associated damages and improvement. Open J. Anim. Sci. 11, 473–500.

Khaeruddin, A.N., Ardi, N., Fattah, A.H., Armayanti, A.K., 2020. Penentuan konsentrasi susu skim terbaik dalam pengencer semen ayam kampung berbahan dasar ringer laktat. J. Vet. 21, 300–308.

Khaeruddin, K., Ciptadi, G., Yusuf, M., Iswati, I., Udrayana, S.B., Wahjuningsih, S., 2024. Effectiveness of butylated hydroxytoluene in maintaining the quality of Gaga chicken sperm in liquid storage for 72 hours. Adv. Anim. Vet. Sci. 12, 371–380.

Khaeruddin, K., Srimaharani, S., 2019. Use of old coconut water with various skim concentrations of milk as a diluent for kampong chicken semen. Chalaza J. Anim. Husb. 4, 24–29.

Kharayat, N.S., Chaudhary, G.R., Katiyar, R., Balmurugan, B., Patel, M., Uniyal, S., Raza, M., Mishra, G.K., 2016. Significance of artificial insemination in poultry. Res. Rev. J. Vet. Sci. Technol. 5, 1–5.

Khunkaew, C., Patchanee, P Panasophonkul, S., Cao, Z., Zhang, Y., Sathanawongs, A., 2021. The sperm longevity and freezability in the modified BHSV extender of Thai Pradu-hangdum chicken. Vet. Integr. Sci. 19, 161–172.

Kim, S.W., Lee, J.Y., Kim, C.L., Ko, Y.G., Kim, B., 2022. Evaluation of rooster semen quality using CBB dye based staining method. J. Anim. Reprod. Biotechnol. 37, 55–61.

Kucera, A.C., Heidinger, B.J., 2018. Avian semen collection by cloacal massage and isolation of DNA from sperm. J. Vis. Exp. 132.

Leão, A.P.A., de Souza, A. V, Mesquita, N.F., Pereira, L.J., Zangeronimo, M.G., 2021. Antioxidant enrichment of rooster semen extenders–a systematic review. Res. Vet. Sci. 136.

Lemoine, M., Mignon-Grasteau, S Grasseau, I., Magistrini, M., Blesbois, E., 2011. Ability of chicken spermatozoa to undergo acrosome reaction after liquid storage or cryopreservation. Theriogenology 75, 122–130.

Lin, Q., Song, B., Zhong, Y., Yin, H., Li, Z., Wang, Z., Cheong, K.., Huang, R., Zhong, S., 2023. Effect of sodium hyaluronate on antioxidant and anti-ageing activities in Caenorhabditis elegans. Foods 12, 1400.

Lotfi, S., Mehri, M., Sharafi, M., Masoudi, R., 2017. Hyaluronic acid improves frozen-thawed sperm quality and fertility potential in rooster. Anim. Reprod. Sci. 184, 204–210.

Masoudi, R., Sharafi, M., Pourazadi, L., 2019. Improvement of rooster semen quality using coenzyme Q10 during cooling storage in the Lake extender. Cryobiology 88, 87–91.

Mavi, G.K., Dubey, P.P., Sahoo, S.K., Grewal, R.S., 2022. Effect of α-tocopherol supplementation in rooster semen on sperm quality parameters during in-vitro storage at 4 °C. Indian J. Anim. Reprod. 43, 43–46.

Najafi, A., Taheri, R.A., Mehdipour, M., Martínez-Pastor, F., Rouhollahi, A.A., Nourani, M.R., 2019. Improvement of post-thawed sperm quality in broiler breeder roosters by ellagic acid-loaded liposomes. Poult. Sci. 98, 440–446.

Pratiwi, N., Yusuf, T.L., Arifiantini, I., Sumantri, C., 2019. Kualitas spermatozoa dalam modifikasi pengencer ringer laktat kuning telur dengan tambahan astaxanthin dan glutathione pada tiga jenis ayam lokal. Acta Vet. Indones. 7, 46–54.

Rad, H.M., Eslami, M., Ghanie, A., 2016. Palmitoleate enhances quality of rooster semen during chilled storage. Anim. Reprod. Sci. 165, 38–45.

Reynolds, S., Ismail, N.F.B., Calvert, S.J., Pacey, A.A., Paley, M.N., 2017. Evidence for rapid oxidative phosphorylation and lactate fermentation in motile human sperm by hyperpolarized 13C magnetic resonance spectroscopy. Sci. Rep. 7, 4322.

Rizkallah, N., Chambers, C.G., de Graaf, S.P., Pickard, J.P., 2022. Factor affecting the survival of ram spermatozoa during liquid storage and options for improvement. Animals 12, 244.

Rui, B.R., Angrimani, D.S., Losano, J.D.A., de Cássia Bicudo, L., Nichi, M., Pereira, R.J., 2017. Validation of simple and cost-effective stains to assess acrosomal status, DNA damage and mitochondrial activity in rooster spermatozoa. Anim. Reprod. Sci. 187.

Santiago-Moreno, J., Blesbois, E., 2020. Functional aspects of seminal plasma in bird reproduction. Int. J. Mol. Sci. 21, 5664.

Silyukova, Y., Fedorova, E., Stanishevskaya, O., 2022. Influence of technological stages of preparation of rooster semen for short-term and long-term storage on its quality characteristics. Curr. Issues Mol. Biol. 44, 5531–5542.

Sun, Y., Xue, F., Li, Y., Fu, L., Bai, H., Ma, H., Xu, S., Chen, J., 2019. Differences in semen quality, testicular histomorphology, fertility, reproductive hormone levels, and expression of candidate genes according to sperm motility in Beijing-You chickens. Poult. Sci. 98, 4182–4189.




DOI: https://doi.org/10.17969/agripet.v25i1.41814

Article Metrics

Abstract view : 0 times
PDF - 0 times

Refbacks

  • There are currently no refbacks.


Copyright (c) Jurnal Agripet

License URL: https://www.jurnal.usk.ac.id/agripet/about/submissions#copyrightNotice

Creative Commons LicenseISSN: 1411-4623E-ISSN: 2460-4534
Copyright© 2000-2025 | ISSN: 1411-4623 | EISSN: 2460-4534 
Jurnal Agripet is licensed under a Creative Commons Attribution 4.0 International License.

 

Published by: 
Animal Husbandry Department, The Faculty of Agriculture, Universitas Syiah Kuala 
associated with Animal Scientist's Society of Indonesia (HILPI). 
Jl. Tgk. Hasan Krueng Kalee No. 3, Kopelma Darussalam,
Banda Aceh, 23111, Indonesia.
Phone: +62-81383736633
Email: jurnalagripet@usk.ac.id

Online Submissions & Guidelines | Editorial Policies | Contact | Statistics | Indexing | Citations