Purifikasi Imunoglobulin Yolk Pada Ayam yang Divaksin terhadap Ekskretori/Sekretori Stadium L3 Ascaridia galli
Abstract
Purification yolk immunoglobulin of hens vaccinated against excretory/secretory Ascaridia galli L3 larvae stage
ABSTRACT. The main immunoglobulin fraction of poultry is called IgY, in order to distinguish it from the mammalian IgG. This article focus on purification yolk immunoglobulin of hens vaccinated against excretory/secretory Ascaridia galli larvae to obtained purity IgY. Active vaccinations with excretory/secretory antigen were applied intra muscularly of chickens with an initial dose of 80 μg. The vaccinations were repeated three times with dose of each 60 μg with an interval of one week. The first vaccinations were excretory/secretory antigen mixed with Fruend Adjuvant Complete and subsequently mixed with Freund Adjuvant Incomplete. Antibody response in yolk was detected at weekly intervals by agar gel precipitation test (AGPT). The chicken’s eggs were collected from 49 day after vaccinations. IgY was extracted from egg yolks by means of ammonium sulphate and purified using fast performance liquid chromatography (FPLC). The purity of anti-ekscretory/secretory IgY protein was determined by Bradford method (λ = 280 nm). The result showed that antibody in yolk was begun detect with AGPT at four weeks after vaccination. IgY concentration after purification was 0,875 ± 0.251 mg/ml. This study has shown that the product released in vitro by L3 stage A. galli is capable of stimulating humoral immunity by mean of producing antibody through yolk as biologic manufacturer could be a good choice.
Keywords
Full Text:
PDFReferences
Akita, E.M., Nakai, S., 1992. Immunoglobulins from Egg Yolk: Isolation and Purification. J. of Food Sci.57: 629 – 634. Amersham. 2003.
Amersham Biosciences, BioDirectory. Amersham Biosciences Ltd.
Camenisch, G., Tini, M., Chilov, D., Kvietikova, I., Srinivas, V., Caro, J., Spielmann,P., Wenger, R.H., Gassmann, M., 1999. General Applicability of Chicken Egg Yolk Antibodies: the Performance of IgY Immunoglobulins Raised Against the Hypoxia-inducible Factor 1. J. FASEB. 13: 81-88.
Carlander, D., 2002. Avian IgY Antibody: in vitro and in vivo. Dissertation, Acta Universitatis Upsaliensis, Uppsala.
Chio, V., 2002. Ducks Antibodies for IVD Applications. IVD Technology. http://www. decifelink.com/ivdt/archive/02/04/003.html. (20-02-2003).
Darmawi, Balqis, U., Tiuria, R., Hambal, M. dan Samadi. 2008. Kajian Titer Antibodi Pada Yolk Dari Ayam Yang Diimunisasi Dengan Antigen Ekskretori/Sekretori Stadium L3 Ascaridia galli. Jurnal Agripet 8(2): 21–26, Tahun 2008
Darmawi, Balqis, U., Tiuria, R., Soejoedono R.D., Pasaribu, F.H., Hambal. 2009. Konsentrasi Protein dan Penentuan Berat Molekul Ekskretori/Sekretori L3 Ascaridia galli. J. Ked. Hewan 3(1): 197 – 202.
Davalos-Patoja, L., Ortego-Vinuesa, J.L., Bastos-Gonzales, D., Hidalgo-Alvarez, R., 2000. Colloidal Stability of IgG and IgY-coated Latex Microspheres, Colloids and Surfaces B: Biointerfaces. 20(2): 165-175.
Haak-Frendscho, M., 1994. Why IgY? Chicken Polyclonal Antibody, an Appealing Alternative. Promega Notes Magazine (46): 11.
Hau, J. and Hendriksen, C.F.M., 2005. Refinement of Polyclonal Antibody Production by Combining Oral Immunization of Chickens with Harvest of Antibodies from the Egg Yolk. J. ILAR. 46(3) (online issues).
Hintz, M., Schares, G., Taubert, A., Geyer, R., Zahner, H., Stirm, S., Conraths, F.J., 1998. Juvenile Female Listomosoides sigmodontis Produce an Excretory-Secretory Antigen (Juv-p120) Highly Modified with Dimethylaminoethanol. J. of Parasitol. 171: 265-271.
Lanyi, B. and Bergan, T., 2003. Bacterial Aglutination. Method in Microbiology, 10: 93 – 168.
Mine, Y., Kovacs-Nolan, J., 2002. Chicken Egg Yolk Antibodies as Therapeutics in Enteric Infectious Disease: A Review. J. Med. Food 5: 159 – 169.
Paryati, S.P.Y., 2006. Antibodi Anti-idiotipe Sebagai Kandidat Vaksin Rabies. Disertasi. Sekolah Pascasarjana Institut Pertanian Bogor.
Rollier, C., Charollois, C., Jamrd, C., Trepo, C., and Cova, L., 2000. Maternally Transferred Antibodies from DNA-Immunized Avians Protect Offspring Against Hepadnavirus Infection. J. of Virol. 74(10): 4908 – 4911.
Schade, R., Henklein, P. and Hlinak, A., 1999. The Production of Avian (Egg Yolk) Antibodies: IgY. The Report and Recommendations of ECVAM Workshop 211,2. Reprinted with Minor Amendments from ATLA 24: 925 - 934.
Schmidt, P., Wiedemann, V., Kühlmann, R., Wanke, R., Linckh, E. and Lösch, U., 1989. Production of Antibodies to Canine Distemper Virus in Chicken Eggs for Immunochemistry. J. of Vet. Med. B 36: 661 – 668.
Soejoedono, R.D., Wibawan, I.W.T, dan Hayati, Z., 2005. Pemanfaatan Telur Ayam Sebagai Pabrik Biologis: Produksi ”Yolk Immunoglobulin” (IgY) Anti Plaque dan Diare dengan Titik Berat pada Anti Streptococcus mutans, Escherichia coli dan Salmonella enterotidis. Laporan Riset Unggulan Terpadu, Kementerian Negara Riset dan Teknologi Republik Indonesia.
Szabo, C.S., Bardos, L., Losonczy, S. and Kachesz, K., 1998. Isolation of Antibodies from Chicken and Quail Eggs. Presented at INABIS ’98 – 5th Internet World Congress on Biomedical Sciences at McMaster University, Canada, December 7 – 16th. http://www.mcmaster.ca/inabis98/immunology/-szabo0509/index.html (20-11-06)
Yoshihara, S., Oya, T., Furuya, T., Goto, N.. 1993. Use of Body Fluid of Adult Female Ascaris suum as an Antigen in the Enzyme-Linked Immunosorbent Assay (ELISA) for Diagnosis of Swine Ascariosis. J. of Helminthol. 67: 279 – 286.
DOI: https://doi.org/10.17969/agripet.v10i2.638
Article Metrics
Abstract view : 0 timesPDF - 0 times
Refbacks
- There are currently no refbacks.
Copyright (c)
Copyright© 2000-2024 | ISSN: 1411-4623 | EISSN: 2460-4534
Jurnal Agripet is licensed under a Creative Commons Attribution 4.0 International License.
Published by:
Animal Husbandry Department, The Faculty of Agriculture, Universitas Syiah Kuala
associated with Animal Scientist's Society of Indonesia (HILPI).
Jl. Tgk. Hasan Krueng Kalee No. 3, Kopelma Darussalam,
Banda Aceh, 23111, Indonesia.
Phone: +62-81383736633
Email: jurnalagripet@usk.ac.id
Online Submissions & Guidelines | Editorial Policies | Contact | Statistics | Indexing | Citations