Pertumbuhan In Vitro dan Ex Vitro Tunas Mikro Tanaman Nilam Aceh (Pogostemon cablin Benth.) dengan Perlakuan Berbagai Konsentrasi Sukrosa dan Arang Aktif

Abstract

In Vitro and Ex Vitro Growth of Plantlet of Patchouli (Pogostemon cablin Bneth.)

ABSTRACT. The objective of this study was to evaluate the effect of sucrose concentration and activated charcoal on the growth shoot and root in vitro and ex vitro of nilam Aceh (Pogostemon cablin Benth.). Explants for this study were ±1 cm in length with 4 leaf, which were derived from sterile shoot culture. The treatment for were cultured on MS basal medium supplemented with sucrose and activated charcoal. The treatment for this study was the concentration of activated charcoal consisting of three levels: 0 (0%), 10 (1%), 20 (2%), 30 (3%), 40 g L^-1 (0.2%). Sucrose concentration of 3% without activated charcoal resulted in the good response based on the number of axillary shoots (2,22 shoots per explants), the length of shoot (5,05 cm), the number of leaf (17,76 leaves per explants), the green leaf level (23,01), the survival shoot (93,33%), the number of root (15,33 root per explants) and the length of root (1,23 cm).