Determination Of Total Flavonoids Extract of White (Magnolia Alba (Dc.) Figlar) Using Spectrophotometry UV-Vis Method

Mastura Mastura, Ulil Amna, Sarah Niaci, Tia Pebiola

Abstract


Flavonoids are a group of secondary metabolites produced by plants that have the potential to influence pharmacological activity. This study aims to determine the total flavonoid content of the ethanol crude extract of white cempaka flowers (Magnollia alba(DC.) Figlar) using the UV-Vis spectrophotometry method. This study used colorimetric and spectrophotometric methods using quercetin as a comparison. The qualitative test research results proved that cempaka putih (Magnolia alba (DC.) Figlar) flowers contain flavonoid coumpound. Then a maximum wavelength of 432 nm is obtained with an absorbance of 0.1236, the absorbance calculation for each quercetin concentration and the sample is calculated using a maximum wavelength of 432 nm. Based on the linear regression equation obtained from the standard quercetin curve, the total flavonoid content of the crude ethanol extract of cempaka putih flowers with 3 different concentration variations, at a concentration of 100 ppm, the results obtained for the total flavonoid content were 276,83 ± 17,7 mgQE/g, 500 ppm. total flavonoid levels of 105,189 ± 8,16 mgQE/g, 1000 ppm obtained total flavonoid levels of 79,115 ± 8,33 mgQE/g.

Keywords


Flavonoids; Exstract; Ethanol; Spectrophotometry UV-Vis; Magnolia alba (DC.) Figlar.

Full Text:

PDF

References


Aksara, R., Weny, J. A. M., dan La, A. Identifikasi Senyawa Alkaloid Dari Ekstrak Metanol Kulit Batang Mangga (Mangifera Indica L). Jurnal Entropi. 2013. Vol 3 (1): 1-6.

Ismawan, B. 2010. Herbal Indonesia Berkhasiat Bukti Ilmiah dan Cara Racik Volume 8..Trubus Swadaya: Bogor.

Ipandi, I., Triyasmono, L., dan Prayitno, B.. Penentuan Kadar Flavonoid Total dan Aktivitas Antioksidan Ekstrak Etanol Daun Kajajahi (Leucosyke capitellata wedd). Jurnal Pharmascience. 2016. Vol. 3, No.1: 99-107.

Pensuk, W., Tanit, P., Chayan, P. Comparison of The Chemical Consistuents in Michelia alba Flower Oil Extracted by Steam Distillation, Hexane Extraction and Enflourage Method. Journal of That Traditional and Alternative Medicinel. 2017. Vol 5. No.1.

Robby, F. A., Iman, S. P., Galuh T. Pediculidical Activity of White Cempaka Flower (Michelia alba DC.). Againts Pediculus Humanus Capitis. Ad-Dawa Journal of Pharmaceutical Sciences. 2021. Vol. 4. No 1: 9-16.

Lan-yu, X., Jia Li, Yanqing, Y., Jiayu, Z., Yongwen, J. Comprehensive Investigation on Flavonoids Metabolites of Longjin Tea in Different Cultivas, Geographical Origins, an Strongetime. Heliyon, 2023, Vol 9.

Harbone, J.B., Metode Fitokimia; Penuntun Cara Modern Menganalisa Tumbuhan. Terbitan Kedua, Terjemahan Kokasih Padmawinata dan Iwang Soeidiro. 1987. Bandung: ITB.

Isnaini, L. Ekstraksi Pewarna Merah Cair Alami Berantioksidan Dari Kelopak Bunga Rosella (Hibicus Sabdriffa L) dan Aplikasinya Pada Produk Pangan. Jurnal Teknologi Pertanian. 2010. Vol 11 (1).

Utami, T. S., Arbianti, R., Hermansyah, H., dan Reza, A. Perbandingan Aktivitas Antioksidan Ekstrak Etanol Daun Simpur (Dillenia indica) dari Berbagai Metode Ekstraksi dengan Uji ANOVA. Prosiding Seminar Nasional Teknik Kimia Indonesia. Bandung: 19-20 Oktober 2009. Hal: 1-6.

Synder, C. R., J.J. Kirkland, and J. L. Glajach. Pratical HPLC Method Develoment, Second Edition. New York: John wiley and Sons. Lnc. 1997. Pp. 722-723.

Oktavia, D. F., Suyatno, S. Skrining Fitokimia, Kandungan Flavonoid Total, Dan Aktivitas Antioksidan Ekstrak Etanol Tumbuhan. Jurnal Kimia Riset. 2021. Vol 6. No 2: 141-153.

Azizah, D. N., Kumolowati, E., Faramayuda, F. Penetapan Kadar Flavonoid Metode AlCl3 Pada Ekstrak Metanol Kulit Buah Kakao (Theobroma cacao L.). Kartika Jurnal Ilmiah Farmasi. 2014. Vol 2 (2): 47.

Chang, C. C., Yang, M. H., Wen, H. M., dan Chern, J.C. Estimation of Total Flavonoid Content in Propolis by Two Complementary Colometric Methods. Journal of Food and Drug Analysis. 2002. Vol 10 (3): 178-182

Saraswati, N. F. Uji aktivitas Antibakteri Ekstrak Etanol 96% Limbah Kulit Pisang Kepok Kuning (Musa balbisiana) Terhadap Bakteri Penyebab Jerawat (Stapylococcus epidermidis, Staphylococcus aureus, dan Propionibacterium acne). Skripsi, 2015.




DOI: https://doi.org/10.24815/jocarbazon.v2i1.37890

Article Metrics

Abstract view : 0 times
PDF - 0 times

Refbacks

  • There are currently no refbacks.


Copyright (c) 2024 Journal of Carbazon

E-ISSN: 2988-5744

 

This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).