A MEDIUM CONTAINING COMMERCIAL FOLIAR FERTILIZER AND SOME ORGANIC ADDITIVES COULD SUBSTITUTE MS MEDIUM FOR IN VITRO GROWTH OF DENDROBIUM HYBRID SEEDLINGS
Abstract
In an attempt to obtain a cost-effective and a simpler way of growing Dendrobium seedling in vitro, this study was conducted to investigate effects of basal media and organic additives on in vitro growth of Dendrobium seedlings. The basal media used were that of half strength Murashige and Skoog (½ MS medium) and that containing foliar fertililizer 32:10:10 and trace elements (FT medium). Treatments were combinations of basal media (1/2 MS medium and FT medium) and different kinds of organic additive (tomato, potato, and mungbean sprout juice and banana homogenate). The experiment was conducted in a completely randomized design with three replicates, 5 culture vessels per replicate and 4 seedlings ca. 1.5 cm per vessel. The experiment showed that FT medium resulted in significantly higher growth than ½ MS medium as indicated by seedling fresh weight. The media containing tomato juice led to highest growth of seedling as shown by seedling fresh weight and seedling height. Since FT medium caused better growth than ½ MS medium, the FT medium could be used as a substitute for ½ MS medium for growing Dendrobium hybrid seedlings. The seedlings were successfully acclimatized in a shaded green house environment with 100% survival rate.
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Abrahamian,P., A. Kantharajah. 2011. Effects of vitamins on in vitro organogenesis of plants. Am. J. Plant Sci. 2(5): 669-674.
Aktar, S., K.M. Nasiruddin, and K. Hossain. 2008. Effects of Different Media and Organic Additives Interaction on In Vitro Regeneration of Dendrobium Orchid. J Agric Rural Dev 6: 69-74.
Al-Khayri, J,M. 2001. Optimization of biotin and thiamine requirements for somatic embryogenesis of date palm (Phoenix dactylifera L.).In Vitro Cell Dev. Biol. Plant. 37 (4):453-456.
David, E., R. Jawan, H. Marbawi, J.A. Gansau. 2015. Organic Additives Improves the in Vitro Growth of Native Orchid Vanda helvola Blume. Not Sci Biol.7(2):192-197.
De Pinto, M.C., D. Francis, L. Gara. 1999. The redox state of the ascorbate-dehydroascorbate pair as a specific sensor of cell division in tobacco By-2 Cells. Protoplasma 209 (1-2):90-97.
Dwiyani, R., H. Yuswanti, I.A.P. Darmawati, K. Suada, and N.N.A. Mayadewi. 2015. In vitro germination and its subsequent growth of an orchid of Vanda tricolor Lindl. var.suavis from Bali on complex additives enriched medium. Agrivita 37(2): 144-150.
Gnasekaran, P., R. Poobathy, M. Mahmood, M. R. Samian, and S. Subramaniam. 2012. Effects of complex organic additives on improving the growth of PLBs of Vanda Kasem’s Delight. AJCS 6(8):1245-1248.
Jawan, R., J.A. Gansau, and J.O. Abdullah. 2010. In Vitro Culture of Borneo’s Endemic Orchid, Vanda dearei. AsPac J. Mol. Biol. Biotechnol 18 (1) : 203-207
Joy, R.W., K.R. Patel, and T.A. Thorpe. 1988. Ascorbic acid enhancement of organogenesis in tobacco callus. Plant Cell, Tissue and Organ Culture 13(3): 219-228.
Kaur, S. and K.K. Bhutani. 2012. Organic growth supplement stimulants for in vitro multiplication of Cymbidium pendulum (Roxb.) Sw. Hort. Sci. (Prague) 39 (1): 47–52
Molnar, Z., E. Virag, and V. Ordog. 2011. Natural substances in tissue culture media of higher plants. Acta Biologica Szegediensis 55(1): 123-127.
Murashige, T. And F. Skoog. 1962. A revised medium for rapid growth and bio assay with tobacco tissue cultures. Physiologia Plantarum. 15(3): 473-497.
Murdad, R., M.A. Latip, Z.A. Aziz, and R. Ripin. 2010. Effects of carbon source and potato homogenate on in vitro growth and development of Sabah’s Endangered orchid: Phalaenopsis gigantea. AsPac J. Mol. Biol. Biotechnol.18 (1) : 199-202.
Muthukrishnan, S., T. S. Kumar, and M. V. Rao. 2013. Effects of different media and organic additives on seed germination of Geodorum densiflorum (Lam) Schltr. – an endangered orchid. IJSR 2(8):23-26.
Parthibhan, S., M. V. Rao, and T.S. Kumar. 2015. In vitro regeneration from protocorms in Dendrobium aqueum Lindley – An imperiled orchid. Journal of Genetic Engineering and Biotechnology 13: 227-233.
Pusat Data dan Sistem Informasi Pertanian. 2015. Outlook Komoditas Pertanian Subsektor Hortikultura: Anggrek. Pusat Data dan Sistem Informasi Pertanian, Sekretariat Jendral Kementerian Pertanian, Republik Indonesia.
Shadang, R., P. Dwivedi, S.N. Hegde, and N. Ahmed. 2007. Effects of different culture media on seed germination and subsequent in vitro development of protocorm of Hygrochilus parishii (Veith&Rchb.f.) Pfitz (Orchidaceae). Indian Journal of Biotechnology 6: 256-261.
Sunitha, S.N. 2014. Regenerative Effect of L-ascorbic acid on the in vitro grown plants. British Biotech. J. 4(12):1238-1252.
The World Healthiest Foods. 2018. Tomatoes: What’s new and beneficial about tomatoes. available at www.whfoods.com , assesed at 27/04/2018.
Thorpe, T.A., C. Stasolla, E.C. Yeung, G.J. de Klerk, A. Roberts, and E.F. George. 2008. The Components of Plant Tissue Culture Media II: Organic Addition, Osmotic and pH Effects, and Support System. In E.F.George, M.A. Hall, and G.J. de Klerk (Eds.) Plant Propagation by Tissue Culture, 3rd Edition, Vol.1. The Background. Springer-Verlag, Dordrect, pp.155-173.
Tomar, S.T., S. Khamba, S. Kaushik, and R.K. Mishra. 2018. Role of vitamins in plant growth and their impact on regeneration of plants under in vitro condition. IJRASET 6: 423-426.
Winarto, B., and J.A.T. da Silva. 2015. Use of coconut water and fertilizer for in vitro proliferation and plantlet production of Dendrobium ‘Gradita 31’. In Vitro Cell Dev.Biol.-Plant 51: 303-314.
Xuan, L.R. 2015. Effect of organic additives on in vitro seed germination of Borneos’s endemic orchid, Vanda dearei. Thesis. Department of Molecular Biology Faculty of Resource Science and Technology Universiti Malaysia Sarawak. 64p.
Zhang, Y. 2013. Biological Role of Ascorbate in Plant in Ascorbic Acid in Plants: Biosynthesis, Regulation and Enhancement. SpringerBriefs in Plant Science. http://www.springer.com/978-1-4614-4126-7. Assesed at July 19, 2018.
DOI: https://doi.org/10.17969/floratek.v13i1.11496
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