PHYLOGENETIC ANALYSIS OF 16S rRNA GENE FRAGMENT OF ANTIBIOTIC-PRODUCING PLS 76 ISOLATE
Abstract
Exploitation of extremophiles as novel bioactive compounds sources has been increasing. The aim of this study was to evaluate the activity and the class of antibiotic produced by a thermo-halophilic isolate PLS 76, as well as to identify the genotype of the isolate. The activity was determined by a disc diffusion method, while the antibiotic class was determined qualitatively by chemical reactions using ninhydrin, iodine vapour and potassium iodine. The genotype was determined by sequencing the 16S rRNA gene fragment and the phylogenetic tree from the sequence data. The results showed that PLS 76 was a Gram-negative bacterium and able to produce polypeptide antibiotic, which showed a slight activity on E. coli and S. aureus. Sequence alignment of the 16S rRNA gene fragment showed that PLS 76 was most related to Geobacillus kaustophilus. These results may be used to utilise the isolated for further antibiotic study.
Keywords
References
Seckbach, J. Oren, A. and Lotter, H.S. 2013. Polyextremophiles Life Under Multiple form of Stress: SpringerLink
Cavicchioli, R. 2002. Extremophiles and The Search for Extraterrestrial Life. Astrobiol. 2 (3): 281-292.
Dalmaso, G.Z.L., Ferreira, D. and Vermelho, A.B. 2015. Review: Marine Extremophiles: A Source of Hydrolases for Biotechnological Applications. Mar. Drugs. 13: 1925-1965.
Widhiastuty, M.P., Febriani, Yohandini, H., Moeis, M.R., Madayanti, F., and Akhmaloka. 2009. Characterization and Identification of Thermostable Alkaline Lipase producing Bacteria From Hot Spring Around West Java, Journal of Pure and Applied Microbiology, 3: (1) 27-40.
Mesbah, N.M., and Wiegel, J., 2012.Life Under Multiple Extreme Conditions : Diversity andPhysiology of The Halophilic Alkalithermophiles, Applied and Environmental Microbiology, 78 (12): 4074-4082.
Ammor, M. S., Rachman, C., Chailou, S., Prevost, H., Xavier, D and Zagorec, M. 2005. Phenotypic and genotypic identification of lactic acid bacteria from a small-scale facility producing traditional dry sausages. Journal Food Microbiology. 22(5).373-382.
Akhmaloka, Suharto,A., Nurbaiti,S., Tika, I.N.,Warganegara, F.M. 2006. Ribotyping identification of Thermophilic Bacterium From Papandayan Creater, Proc ITB Eng Science, 38B (1),1-10.
Iqbalsyah, T.M., Yusniar, Sartika, F., Febriani, 2006. A local Isolate of PLS 80 Capable of Producing a β-Lactams Antibiotic, The 6th Annual International Conference (AIC) , Banda Aceh.
Nabila, J.P., Febriani, Iqbalsyah, T.M., 2014. Screening of α-Amylase Production Ability of Four Thermo-Halophilic Bacteria Isolated From An Underwater Hot Spring In Pria Laot Sabang, ICONES FMIPA Unsyiah .
Atikah,Oesman, F., Iqbalsyah, T.M Febriani, 2014. Screening of Four Thermo-Halophilic Bacteria Isolated From Underwater Hot Spring in Pria Laot Sabang In Producing Proteases, ICONES FMIPA Unsyiah.
Gregoire, P., Bohli, M., Cayol, L. J., Joseph, M and Guasco, S. 2011. Caldilinea tarbalica sp. Nov., a Filamentous, thermophilic, anaerobic bacterium isolated from a deep hot aquifer in the aquitaine basin. Internasional Journal of Systematic and Evolutionary Microbiology, 61,1436-1441.
Park, H. M., Traiwan, J., Jung, Y. M and Kim, W. 2012. Gulosibacter chungangensis sp. Nov., an Actinomycete isolated from a marine sediment and emended description of the genus gulosibacter. Internasional Journal of Systematic and Evolutionary Microbiology. 62, 1055-1060.
He, S., Wang, H., Yan, X., Zhu, P., Chen, J. and Yang, R., 2013, Preparatife isolation and purification of macrolactin antibiotics from marine bacterium Bacillus amyloliquefaciens using high-speed counter-current chromatography in stepwise elution mode, Journal of Chromatography A, China. 1272 – 15-19.
Oku, N., Kawabata, K., Adachi, K., Katsuta, A., Shizuri, Y. 2009. Unnarmicins A and C, new antibacterial depsipeptides produced by marine bacterium photobacterium sp. MBIC06485. The Journal Of Antibiotics. Japan Antibiotics Research Association. 61(1): 11–17.
Bauer AW, Kirby WM, Sherris JC and Turck M 1966 Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45 (4) 493–496.
Hancu G, Simon B, Kelemen H, Rusu A, Mircia E and Gyeresi A 2013. Thin layer chromatographic analysis of Beta-lactam antibiotics. Adv. Pharma. Bull. 3(2) 367-371.
Mohandass, C., Rajasabathy, R., Ravindran, C., Colaco, A., Santos, R. & Meena, R. M.. (2012). Bacterial Diversity and Their Adaptations in The Shallow Water Castro Seamount (DJCS), Azores, Portugal. Cah. Biol. Mar. 53 : 65-76.
Iqbalsyah TM, Saidi N, Maulyna N, Helwati H, Aura N, Febriani. 2016. Skrining antibiotik dari bakteri thermo-halofilik isolat Pria Laot Sabang (PLS A dan 76). Prosiding Seminar Nasional XX Perhimpunan Biokimia Dan Biologi Molekuler Indonesia (PBBMI) 47-55.
Febriani, Ramayanti, Iqbalsyah, T.M, Khairan, Oesman, F., 2014. Antibiotic production From Thermophilic Jaboi Sabang Local Isolate Using TSB Medium, Proceeding of Aceh International Pharmacy Conference. 43-47.
Kumar, A., Saini, P., Shrivastava, J.N., 2009. Production of Peptide antifungal antibiotic and Biocontrol Activity of Bacillus subtilis. Indian Journal of Experimental Biology, 47: 57-62.
Venugopalan, V.,Singh, B., Verma, N., Nahar,P.,Bora, T.C., Das, R.H., Gautam, H.K., 2008. Screening of Thermophilis From Municipal Solid Waste and Their Selective Antimicrobial Profile, Current Research in Bacteriology1 (1): 17-22.
El-Damerdash HA 2012. A Simple and inexpensive procedure for chromosomal DNA extraction from Streptococcus thermophilus Strains. Middle-East J. Sci. Res. 11 (1) : 13-18.
Baker, G.C., Smith, J.J., Cowan, D.A. 2003. Review and Re-analysis of Domain-Spesific 16S Primers (Review Article). J. Microbiol. Meth. 55 (3) : 541-555.
Nei M and Kumar S 2000 Molecular evolution and phylogenetics. Oxford University Press, Inc. New York.
Drancourt M, Bollet C, Carlioz, Martelin R, Gayral JP, Raoult D 2000 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates, J. Clin. Microbiol. 38 3623-3630.
DOI: 10.24815/jn.v18i3.11152
Refbacks
- There are currently no refbacks.
Universitas Syiah Kuala (recognizedly abbreviated as