Biodegradation of Diethyl Phthalate (DEP) Compound Using Fungi Isolated from Leachate Water of Tpa Sarimukti Bandung

Suci Keiva Mulyana*, Emenda Sembiring


Industrial development in Indonesia increases every year. Industries that grow significantly every year are the food, beverage and household furniture industries, which almost all of their packaging uses plastic materials. One of the dangerous and toxic materials in plastic manufacturing is phthalate. One phthalate compound that is commonly found in everyday life is diethyl phthalate (DEP). This compound is dangerous because it is carcinogenic, xenoestrogenic and its effect on the endocrine disrupts the binding and action of natural hormones, thus disrupting physiological processes. The accumulation of plastic in the landfill causes this compound to be identified in leachate with an existing concentration of 2.4 mg/L. Biological treatment is one of the appropriate technologies in DEP removal. The treatment is carried out by removing DEP using fungus isolated in the leachate water sampled. The mechanism of phthalate degradation by fungi can be explained simply where fungi use phthalates as the only source of carbon and energy for their growth. Degradation tests were carried out on Malt Extract Broth (MEB) liquid media to which phthalate was added at existing concentrations and using selected efficient fungi over a period of 6 days. Concentration testing was carried out by analysis using High Performance Liquid Chromatography (HPLC). Analysis of fungal cell count using spectrophotometric method by measuring OD value. Tests were carried out on days 0, 2, 4 and 6; Fungi from leachate water that have the potential to degrade DEP compounds include Aspergillus Niger, Penicillium sp and Trichoderma sp


Isolat Jamur, Biodegradasi, Phthalate, Diethyl Phthalate, Air lindi

Full Text:



Afdal, A., & Sari, R. N. (2017). Karakteristik Lindi dari Tempat Pembuangan Sampah Akhir (TPA) Air Dingin, Kota Padang, Sumatera Barat. In Prosiding SNFA (Seminar Nasional Fisika dan Aplikasinya) (Vol. 1, p. 8).

Aguilar-Alvarado, Y., Báez-Sánchez, M. del R., Martínez-Carrera, D. C., Ahuactzin-Pérez, M., Cuamatzi-Muñoz, M., & Sánchez, C. (2015). Mycelial growth and enzymatic activities of fungi isolated from recycled paper wastes grown on Di (2-ethylhexyl) phthalate. Polish Journal of Environmental Studies, 24(5), 1897–1902.

Ahmadi, E., Yousefzadeh, S., Ansari, M., Ghaffari, H. R., Azari, A., Miri, M., Mesdaghinia, A., Nabizadeh, R., Kakavandi, B., Ahmadi, P., Badi, M. Y., Gholami, M., Sharafi, K., Karimaei, M., Ghoochani, M., Brahmand, M. B., Mohseni, S. M., Sarkhosh, M., Rezaei, S., … Taghipour, N. (2017). Performance, kinetic, and biodegradation pathway evaluation of anaerobic fixed film fixed bed reactor in removing phthalic acid esters from wastewater. Scientific Reports, 7(December 2016), 1–14.

Ahuactzin-Pérez, M., Tlecuitl-Beristain, S., García-Dávila, J., González-Pérez, M., Gutiérrez-Ruíz, M. C., & Sánchez, C. (2016). Degradation of di(2-ethyl hexyl) phthalate by Fusarium culmorum: Kinetics, enzymatic activities and biodegradation pathway based on quantum chemical modelingpathway based on quantum chemical modeling. Science of the Total Environment, 566–567(May), 1186–1193.

Ayeni, T. O., Arotupin, D. J., & Ayo, O. E. (2022). Biodegradation of polyethylene by indigenous fungi from waste recycling site, South West, Nigeria. Bulletin of the National Research Centre, 46(1).

Begum, A., Katsumata, H., Kaneco, S., Suzuki, T., & Ohta, K. (2003). Biodegradation of phthalic acid esters by bakery yeast Saccharomyces cerevisiae. Bulletin of Environmental Contamination and Toxicology, 70(2), 255–261.

Esfandian, H., Yousefi, E., & Sharifzadeh Baei, M. (2016). Removal of dimethyl phthalate from aqueous solution by synthetic modified nano zeolite using Cu2O nanoparticles. International Journal of Engineering, Transactions A: Basics, 29(9), 1198–1207.

González-Márquez, A., Loera-Corral, O., Santacruz-Juárez, E., Tlécuitl-Beristain, S., García-Dávila, J., Viniegra-González, G., & Sánchez, C. (2019a). Biodegradation patterns of the endocrine disrupting pollutant di(2-ethyl hexyl) phthalate by Fusarium culmorum. Ecotoxicology and Environmental Safety, 170(December 2018), 293–299.

González-Márquez, A., Loera-Corral, O., Santacruz-Juárez, E., Tlécuitl-Beristain, S., García-Dávila, J., Viniegra-González, G., & Sánchez, C. (2019b). Biodegradation patterns of the endocrine disrupting pollutant di(2-ethyl hexyl) phthalate by Fusarium culmorum. Ecotoxicology and Environmental Safety, 170(November 2018), 293–299.

Gyung Yoon, M., Jeong Jeon, H., & Nam Kim, M. (2012). Biodegradation of Polyethylene by a Soil Bacterium and AlkB Cloned Recombinant Cell. Journal of Bioremediation & Biodegradation, 03(04).

Kim, Y. H., Lee, J., & Moon, S. H. (2003). Degradation of an endocrine disrupting chemical, DEHP [di-(2-ethylhexyl)- phthalate], by Fusarium oxysporum f. sp. pisi cutinase. Applied Microbiology and Biotechnology, 63(1), 75–80.

Kumar Sen, S., & Raut, S. (2015). Microbial degradation of low density polyethylene (LDPE): A review. Journal of Environmental Chemical Engineering, 3(1), 462–473.

Lee, Soo-Min, Jae-Won Lee, Bon-Wook Koo, Myung-Kil Kim, Don-Ha Choi, I.-G. C. (2007). Dibutyl Phthalate Biodegradation by the White Rot Fungus, Polyporus brumalis. Journal of Bioengineering, 97.

Okoli, C. P., Adewuyi, G. O., Zhang, Q., Diagboya, P. N., & Guo, Q. (2014). Mechanism of dialkyl phthalates removal from aqueous solution using γ-cyclodextrin and starch based polyurethane polymer adsorbents. Carbohydrate Polymers, 114, 440–449.

Robert, B., & Brown, E. B. (2004). Final toxicity review for diethyl phthalate (DEP). 1, 1–14.

Sivan, A. (2011). New perspectives in plastic biodegradation. Current Opinion in Biotechnology, 22(3), 422–426.

Usha, R., Sangeetha, T., & Palaniswamy, M. (2011). Screening of polyethylene degrading microorganisms from garbage soil. Libyan Agriculture Research Center Journal International, 2(4), 200–204.

Wang, H., Sun, D. Z., & Bian, Z. Y. (2010). Degradation mechanism of diethyl phthalate with electrogenerated hydroxyl radical on a Pd/C gas-diffusion electrode. Journal of Hazardous Materials, 180(1–3), 710–715.

Weaver, J. A., Beverly, B. E. J., Keshava, N., Mudipalli, A., Arzuaga, X., Cai, C., Hotchkiss, A. K., Makris, S. L., & Yost, E. E. (2020). Hazards of diethyl phthalate (DEP) exposure: A systematic review of animal toxicology studies. Environment International, 145(September), 105848.

Westerhoff, P., Yoon, Y., Snyder, S., & Wert, E. (2005). Fate of endocrine-disruptor, pharmaceutical, and personal care product chemicals during simulated drinking water treatment processes. Environmental Science and Technology, 39(17), 6649–6663.

Wicaksana, A., & Rachman, T. (2018). BIODEGRADASI LIMBAH PLASTIK OLEH MIKROORGANISME. Angewandte Chemie International Edition, 6(11), 951–952., 3(1), 10–27.

Wijaya, B. A., & Trihadiningrum, Y. (2019). Meso- and microplastics pollution in Surabaya River on Driyorejo to Karang Pilang Segment (in Bahasa). Jurnal Teknik ITS, 8(2), 211–216.


Article Metrics

Abstract view : 0 times
PDF - 0 times


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Riwayat: Educational of History and Humanities indexed by


Riwayat: Educational of History and Humanities

E-ISSN 2775-5037
P-ISSN 2614-3917

Published by History Education Department, Faculty of Teacher Training and Education, Universitas Syiah Kuala, Province Aceh. Indonesia
W :
E :

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.