Clinical application of acellular matrix derived from the bubaline diaphragm and caprine rumen for the repair of abdominal wall defects in animals
Abstract
The abdominal wall hernias resulting due to trauma or other clinical conditions are common in animals. Large hernias required the use of synthetic mesh, which is costly and may result in infection, fistula formation, and pain. Application of biomaterials in hernia repair causes a reduction in pain, reduced recovery time, and rate of recurrence. The study was undertaken to test the acellular bubaline diaphragm matrix (BDiaM) and acellular caprine rumen matrix (CRuM) for the repaired hernia in clinical cases. Fresh bubaline diaphragm and caprine rumen were decellularized using sodium deoxycholate (1% for CRuM and 2% for BDiaM) for 48h. Acellularity was ascertained histologically and by DNA quantification. Histologically, both the matrices showed complete acellularity and orderly arranged collagen fibers after 48 h. The DNA contents were significantly (P<0.05) reduced in both the matrices in comparison to the native matrices. The BDiaM and CRuM matrices were applied in eight and nine clinical cases of abdominal wall defects, respectively. Animals with BDiaM and CRuM matrices recovered uneventfully and remained sound at least up to 3 months. Hematological and immunological findings were unremarkable. BDiaM and CRuM matrices showed good results without complications.
Keywords:Biocompatibility, Bubaline diaphragm matrix, Caprine rumen matrix, DNA quantification, ELISA, SDS-PAGE
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Abouelnasr, K.S., A.E. Zaghloul, G.I. Karrouf. 2014. Comparative evaluation of glycerolized bovine pericardium implant with prolene mesh for closure of large abdominal wall defects in dogs. Iranian Journal of Veterinary Research, 15(3): 211-217.
Amid, K.P. 1997. Classification of biomaterials and their related complications in abdominal wall hernia surgery. Hernia, 1: 15.
Badylak, S.F. 2004. Xenogeneic extracellular matrix as a scaffold for tissue reconstruction. Transplant Immunology, 12: 367–77.
Badylak, S. F. 2007. The extracellular matrix as a biologic scaffold material. Biomaterials,28: 3587-3593.
Bigbie, R.B., J. Schumacher, S.F. Swain, K.C. Purohit, J.S. Wright. 1991. Effect of amnion and yeast cell derivatives on second intention healing in horses. American Journal of Veterinary Research, 52: 1376.
Borschel, G.H., R.G. Dennis, J.W.M. Kuzon. 2004. Contractile skeletal muscle tissueengineered on an acellular scaffold. Plastic and Reconstructive Surgery,113: 595–602.
Butler, C.E. 2006. The role of bioprosthetics in abdominal wall reconstruction. Clinics in Plastic Surgery, 33: 199-211.
Clarke, K.M., G.C. Lantz, S.K. Salisbury, S.F. Badylak, M.C. Hilesand, S.L. Voytik. 1996. Intestine submucosa and polypropylene mesh for abdominal wall repair in dogs. Journal of Surgical Research, 60: 107-114.
Courtman, D.W., B.F. Errett, C.A. Pereira, V. Kashef, D. McComb, J.M. Lee, G.J. Wilson. 1994. Development of pericardial acellular matrix biomaterial: Biochemical and mechanical effects of cell extraction. Journal of Biomedical Materials Research, 28: 655–666.
Devarathnam, J., A.K. Sharma, R.B. Rai, S.K. Maiti, S.S. Sonal, N. Kumar. 2014. In vitro biocompatibility determination of acellular aortic matrix of buffalo origin. Trends in Biomaterials and Artificial Organs, 28(3): 92-98.
Dewangan, R., A.K. Sharma, N. Kumar, A. Kumar, H. Singh, V. Kumar, S. K. Maiti, S.S. Sonal, R. Singh. 2013. Efficacy of bladder acellular matrix graft with and without cell seeding for cystoplasty in rabbits. Trends in Biomaterials and Artificial Organs, 27(2): 47-57.
Dewangan, R., A.K. Sharma, N. Kumar, S.K. Maiti, H. Singh, A. Kumar, S.S. Sonal, R. Singh. 2012. In-vivo determination of biocompatibility of bladder acellular matrix in a rabbit model. Trends in Biomaterials and Artificial Organs, 26: 43-55.
Eid, G.M., J.M. Prince, S.G. Mattar, G. Hamad, S. Ikrammudinand, P.R. Schauer. 2003. Medium-term follow-up confirms the safety and durability of laparoscopic ventral hernia repair with PTFE. Surgery, 134: 599-603.
Farquharson, J. 1946. Hernia in horses and cows. Journal of American Veterinary Medical Association, 108: 409-411.
Gangwar, A.K., A.K. Sharma, S.K. Maiti, N. Kumar. 2004. Xenogenicacellular dermal graft for the repair of ventral hernia in a non-descript goat. The Indian Veterinary Medical Journal, 28: 95-96.
Gilbert, T.W., T.L. Sellaroand, S.F. Badylak. 2006. Decellularization of tissues and organs. Biomaterials, 27: 3675-3683.
Gulati, A.K., G.P. Cole. 1994. Immunogenicity and regenerative potential of acellular nerve allograft to repair a peripheral nerve in rats and rabbits. Acta Neurochirurgica, 126: 158-164.
Iqbal, M., I. Isma, M.A. Khanand, I.G. Azim, F. Ahmed. 1994. Reconstruction of abdominal wall defect with a jejunal graft in dogs. International Journal of Animal Sciences, 9:147.
Kaarthick, D.T., A.K. Sharma, N. Kumar, V. Kumar, A.K. Gangwar, S.K. Maiti, M. Negi, V.Remya, D.D. Mathew, J. Devarathnam, S.S. Sonal, R. Singh. 2017. Accelerating full-thickness dermal wound healing using primary mouse embryonic fibroblasts seeded bubaline acellular diaphragm matrix. Trends in Biomaterials and Artificial Organs, 31(1):16-23.
Khan, M.A., T. Hamid, A.K. Mahmood, U. Farooq, S.S. Ahmadand M.K. Shahzad. 2008. Comparative efficacy of polypropylene mesh and jejunal graft for the repair of abdominal wall defect in dogs. Journal of Animal and Plant Sciences, 18(2-3): 67–71.
Kropp, B.P., B.L. Eppley, C.D. Prevel, M.K. Rippy, R.C. Harruff, S.F. Badylak. 1995. Experimental assessment of small intestinal submucosa as a bladder wall substitute. Urology, 46: 396–400.
Kumar, V., A.K. Gangwar, N. Kumar. 2016. Evaluation of the Murine Dermal Matrix as a Biological Mesh in Dogs. Proceedings of the National Academy of Sciences India, Section B Biological Science, 86:953-960.
Kumar, V., A.K. Gangwar, N. Kumar, H. Singh. 2015. Use of the bubaline acellular diaphragm matrix for umbilical hernioplasty in pigs. Veterinarski Arhiv, 85: 49-58.
Kumar, V., J. Devarathnam, A.K. Gangwar, N. Kumar, A.K. Sharma, A.M. Pawde, H. Singh. 2012. Use of acellular aortic matrix for reconstruction of abdominal hernias in buffaloes. Veterinary Record, 170: 392.
Kumar, V., N. Kumar, H. Singh, A.K. Gangwar, R. Dewangan, A. Kumar, R. B. Rai. 2013a. In vitro evaluation of bubaline acellular small intestinal matrix. International Journal of Bioassays, 2(3): 581-587.
Kumar, V., N. Kumar, H. Singh, A.K. Gangwar, R. Dewangan, A. Kumar, R.B. Rai. 2013b. Effects of crosslinking treatments on the physical properties of acellular fish swim bladder. Trends in Biomaterials and Artificial Organs, 27(3): 93-101.
Kumar,V., N. Kumar, A.K. Gangwar, A.C. Saxena. 2013. Using the acellular aortic matrix to repair umbilical hernias of calves. Australian Veterinary Journal, 91(6): 252-253
Kumar,V., N. Kumar, A.K. Gangwar, H. Singh, R. Singh. 2015. Comparative histologic and immunologic evaluation of 1,4-butanediol diglycidyl ether crosslinked versus noncrosslinkedacellular swim bladdermatrix for healing of full-thickness skin wounds in rabbits. Journal of Surgical Research, 197(2): 436-446.
Ladurner, R., A. Trupka, S. Schmidbauerand K. Hallfedt. 2001. The uses of an underlay polypropylene mesh in complicated incisional hernias: successful French surgical technique. Minerva Chirurgica, 56: 111.
Laschke, M., W.B. Vollmarand, M. D. Menger. 2009. Inosculation: connecting the life sustaining pipelines. Tissue Engineering Part B: Reviews, 15: 455-465.
Lee, C. H., A. Singlaand, Y. Lee. 2001. Biomedical application of collagen. International Journal of Pharmacology, 221: 21-22.
Mathew, D.D., V. Remya, S.K. Maiti, A.K. Sharma, N. Kumar. 2017. Perinealhernioplasty using murine acellular dermal matrix as a biological mesh in a dog. Indian Journal of Canine Practice, 9(2): 155-157
Mohsina, A., A.K. Gangwar, D.D. Mathew, V. Remya, S.K. Maiti, A.K. Sharma, N. Kumar. 2013. Umbilical hernioplasty using decellularised rabbit skin in a buffalo calf. XXXVII annual congress of ISVS, 6-8 November, 2013, Mannuthy, Thrissur, Kerala, Abstract in Large Animal Surgery (Poster), Page 235.
Ober, C., A. Muste, L. Oana, N. Mates, F. Beteg, S. Veres. 2008. Using of prosthetic biomaterials in large animals: modern concepts about abdominal wall defects approach. Journal of Central European Agriculture, 9: 575-580.
Pellegata, A.F., M. Adelaide Asnaghi, I. Stefani, A. Maestroni, S.Maestroni T. Dominioni, S. Zonta, G. Zerbini, S. Sara Mantero. 2013. Detergent-enzymatic decellularization of swine blood vessels: insight on mechanical properties for vascular tissue engineering. BioMed Research International, 2013: 918753.
Perme, H., A.K. Sharma, N. Kumar, H. Singh, S.K. Maiti, R. Singh. 2009. In-vivo biocompatibility evaluation of crosslinked cellular and acellular bovine pericardium. Indian Journal of Animal Sciences, 79: 658-661.
Raghuvanshi, P.D.S., D. Mohan, D. Gautam, S. Shivaraju, S.K. Maiti, N. Kumar. 2018. Clinical application of animal based extracellular matrix in hernioplasty. MOJ Imunology, 6(4):115-118.
Rathore, H.S., P.D.S. Raghuvansi, D. Gautam, D. Mohan, A.K. Singh, A.P. Singh, S.K. Maitiand, N. Kumar. 2018. Use of the bubaline acellular diaphragm matrix (ADM) for repair of abdominal wall defects in four different species of animals. International Journal of Chemical Studies, 6(4): 157-161.
Remya V., N. Kumar, A.K. Sharma, Sonal, M. Negi, S.K. Maiti, D.D. Mathew, S. Shrivastava, N.P. Kurade. 2014. Acellular Fish Swim Bladder Biomaterial Construct Seeded with Mesenchymal Stem Cells for Full Thickness Skin Wound Healing in Rats. Trends in Biomaterials and Artificial Organs, 28(4): 127-135.
Remya V., P. Shakya, T.B. Sivanarayanan, P. Dubey, A.M. Pawde, N. Kumar. 2013. Umbilical hernioplasty using acellular bovine diaphragm matrix in a calf. Ruminant Science, 2(2): 229-231.
Remya, V., N. Kumar, A.K. Sharma, D.D. Mathew, M. Negi, S.K. Maiti, S.S.Sonal, N.P. Kurade. 2014. Bone marrow derived cell-seeded extracellular matrix: A novel biomaterial in the field of wound management. Veterinary World, 7: 1019-1025.
Schmidt, C.E., J.M. Baier. 2000. Acellular vascular tissue: natural biomaterials for tissue repair and tissue engineering. Biomaterials, 21: 2215–31.
Schumpelick, V., U. Kling, K. Junge, M. Stumpf. 2004. Incisional abdominal hernia: the open mesh repair. Langenbecks Archives of Surgery, 389: 1-9.
Shakya, P., D.D. Mathew, V. Remya, A. Mohsina, A.K. Sharma, S.K. Maiti, N. Kumar. 2013. Acellular diaphragm matrix for the repair of umbilical hernia in two cow calves. XXXVII annual congress of ISVS, 6-8 November, 2013, Mannuthy, Thrissur, Kerala, Abstract in Ruminant Surgery, Page 189.
Singh, A.K., N. Kumar, S. Shrivastava, K.P. Singh, S. Dey, P.D.S. Raghuwansi, A.P. Singh. 2018. Evaluation of the efficacy of bubaline rumen derived extracellular matrix in umbilical hernioplasty in buffalo calves. International Journal of Fauna and Biological Studies, 5(1): 29-33.
Snedecor, G.W., W.G. Cochran. 1990. Statistical Methods, edn 7th Iowa State University Press, Iowa, USA.
Vilar, J.M., F. Doreste, G. Spinella, S. Valentin. 2009. Double layer mesh hernioplasty for repair of incisional hernias in 15 horses. Journal of Equine Veterinary Science, 29: 131–76.
Ward, B.R., K.D. Johnson, B.C.H. May. 2014. Tissue scaffolds derived from forestomach extracellular matrix. U.S. Patent 8,758,781.
DOI: https://doi.org/10.13170/ajas.4.2.13071
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