Blood profiles of African catfish Clarias gariepinus fingerlings fed diets supplemented with three strains of Lactobacillus plantarum

Daniel Olusegun Diyaolu, Emmanuel A. Fasakin, Olabode T. Adebayo

Abstract


A 10-week feeding trial was carried out to evaluate strain-specific effects of Lactobacillus plantarum on blood profiles of African catfish Clarias gariepinus fingerlings. A catfish diet was formulated and divided into five portions (experimental diets). The first portion served as control (with no probiotic added to it, labeled as NP) while the second portion received 2.5g kg-1 multi-species commercial probiotics (CP). The third, fourth and fifth portions were the experimental diets that contained 2×109cfu/g of L. plantarum strains of ascension numbers LC333558, LC333559 and LC333560, and labeled as LP58, LP59 and LP60, respectively. Fifteen apparently healthy C gariepinus fingerlings (4.86±0.59g) were stocked in an experimental tank of 70 litres capacity, while the experimental feed was fed to the fish in triplicate tanks. Similar rearing conditions were maintained in all experimental tanks. The results showed significant (p<0.05) difference between the fish fed control diet and probiotics supplementation diets in some of the haematological parameters examined. Probiotics supplementations did not affect total and differential leukocytes among different experimental groups. Except for RBC counts, fish groups fed with strains LB59 and LB60 had similar effects and appeared to have better haematological values than groups fed with LB58. Strains-specific effect of L. planatrum was also observed in creatinine levels among the experimental groups. Hence, it can be concluded that strains of L. plantarum affected some haematological parameters of C. gariepinus fingerlings positively, while dietary supplementation of L. plantarum LC333559 strain could be employed to improve haematological parameters of C. gariepinus fingerlings.

Full Text:

PDF

References


Adeniyi, O.V., F.E. Olaifa, B.O. Emikpe, S.T. Ogunbanwo. 2021. Effects of Tamarindus indica (Linnaeus 1753) pulp-fortified diets on the gut microflora and morphometry in African catfish Clarias gariepinus (Burchell 1822). Aceh Journal of Animal Science, 6 (2): 45 – 51.

Aderolu, A.Z., M.O. Lawal, L.I. Okoronkwo, F.O. Awobajo, F.I. Jesuniyi. 2017. Dietary effect of Cissus populnea and Securidaca longepedunculata aqueous leave extracts on reproductive, haematological and biochemical parameters of African catfish, Clarias gariepinus (Burchell, 1822) broodstocks. Aceh Journal of Animal Science, 2(1): 1-11.

Aitken M.M., E. Hall, W. M. Allen, L. Scott, J. L. Devot. 2003. Liver- related biochemical changes in the serum of dogs being treated with phenobarbitone. Veterinary Record, 153: 13-16.

Ajeniyi. S. A., R. J. Solomon. 2014. Urea and creatinine of Clarias gariepinus in three different commercial ponds. Nature and Science 12(10): 124-138.

Akintayo, I.A., S. O. Obasa, W. O. Alegbeleye, A. N. Bangbose. 2008. Evaluation of toasted sunflower (Helianthus annus) seed meal in the diets of African catfish (Clarias gariepinus) fingerlings. Livestock Research and Rural Development, 20: 28-46.

Al-Dohail, M. A., R. Hashim, M. Aliyu-Paiko. 2009. Effects of the probiotic, Lactobacillus acidophilus, on the growth performance, haematology parameters and immunoglobulin concentration in African Catfish (Clarias gariepinus, Burchell 1822) fingerling. Aquaculture Research, 40: 1642-1652

Al-Dohail, M. A., R. Hashim, M. Aliyo-Paiko. 2011. Evaluating the use of Lactobacillus acidophilus as a bio-control agent against common pathogenic bacteria and the effects on the haematology parameters and histopathology in African catfish Clarias gariepinus juveniles Aquaculture Research 42: 196 – 209.

Aureli P., L. Capurso, A. M. Castellazzi, M. Clerici, M. Giovannini, L. Morelli, A. Poli, F. Pregliasco, F. Salvini, G. V. Zuccotti. 2011. Probiotics and health: An evidence-based review. Pharmacology Research 63: 366 – 376.

Bahmani, M., R. Kazemi, P. Donskaya. 2001. A Comparative study of some hematological features in young reared sturgeons (Acipenser persicus and Huso huso). Fish Physiology and Biochemistry, 24: 135-140.

Batista, S., R Ozório, S. Kollias, A. Dhanasiri, J. Lokesh, V. Kiron, V. LMP, J. Fernandes 2016. Changes in intestinal microbiota, immune- and stress-related transcript levels in Senegalese sole (Solea senegalensis) fed plant ingredients diets intercropped with probiotics or imunostimulants. Aquaculture, 458: 149-157

Blaxhall, P.C., K. W. Daisley. 1973. Routine haematological methods for use with fish blood. Journal of Fish Biology, 5: 771–781.

Boyle R. J., R. M. Robins-Browne, M. L. Tang. 2006. Probiotic use in clinical practice: what are the risks? American Journal of Clinical Nutrition, 83: 1256-1264.

Brown, B.A. 1993. Routine hematology procedures. Hematology: Principles and Procedures. Lippincott Williams and Wilkins Publisher, Philadelphia.

Brunt J., R. Hansen, D. J. Jamieson, B. Austin .2008. Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets. Veterinary Immunology and Immunopathology, 121: 199-205.

Dacie, J.V., S.M. Lewis. 2001. Practical haematology. 9th Edition, Churchill Livingstone, London, 633.

Diyaolu, D.O., F. O. Ekundayo, E. A. Fasakin, O. T. Adebayo. 2018. Phenotypic and molecular characterization of different isolates of Lactobacillus plantarum from four Nigerian fermented foods for use as probiotics in aquaculture. Brazilian Journal of Biological Sciences, 5 (10): 329-337.

Douglass, J. W., K. W. Jane. 2010. Schalm’s veterinary haematology. John Wiley and Sons. Blackwell Publishing Ltd., New Jersey.

Edsall, C. C. 1999. A blood chemistry profile for Lake Trout. Journal of Aquatic Animal Health, 11 (1): 81- 86.

Eisler R. 1965. Some effects of a synthetic detergent on estuarine fishes. Transactions of the American Fisheries Society, 94 (1): 26 – 13.

Fagbenro O A, E. O. Adeparusi, W. O. Jimoh 2013. Haematological profile of blood of african catfish (Clarias gariepinus, Burchell, 1822) fed sunflower and sesame meal based diets. Journal of Fisheries and Aquatic Sciences, 8: 80-86.

Gatesoupe, F.J. 1999. The use of probiotics in aquaculture. Aquaculture, 180: 147–165.

Gomez-Gil, B., A. Roque, J. F. Turnbull. 2000. The use and selection of probiotic bacteria for use in the culture of larval organisms. Aquaculture, 191: 259–270.

Hirano, Y., M. Aziz, P. Wang. 2016. Role of reverse transendothelial migration of neutrophils in inflammation. Biological Chemistry, 397(6): 497e506.

Hoseinifar, A, A. Mirvaghefi, M. A. Amoozegar, D. L. Merrifield, E. Ringo. 2017. In vitro selection of a symbiotic and in vivo evaluation on intestinal microbiota, performance and physiological response of rainbow trout (Oncorhynchus mykiss) fingerlings. Aquaculture Nutrition, 23: 111 – 118.

Leblanc, J. G., J. E. Laino, M. J. del Valle, V. Vannini, D. van Sinderen, M. P. Taranto. 2011. B-group vitamin production by lactic acid bacteria–current knowledge and potential applications. Journal of Applied Microbiology, 111 (6):1297–309.

Marimuthu, K., H. Palaniandy, Z.A. Muchlisin. 2019. Effect of different water pH on hatching and survival rates of African catfish Clarias gariepinus (Pisces: Clariidae). Aceh Journal of Animal Science, 4(2): 80-88.

Merrifield, D. L., A. Dimitroglou, A. Foey, S. J. Davies, R. T. M. Baker, J. Bøgwald, M. Castex, E. Ringo 2010. The current status and future focus of probiotic and prebiotic applications for salmonids. Aquaculture, 302: 1–18.

Myburgh, J. G., C. J. Botha, D. G. Booye, F. Ryers. 2008. Provisional clinical chemistry parameters in the African sharptooth catfish (Clarias gariepinus). Journal of South African Veterinary Association 79(4):156–160.

Nayak, S. K. 2010. Role of gastrointestinal microbiota in fish. Aquaculture Research, 41: 1553–1573.

Nwanna, L. C., H. Tope-Jegede. 2017. Effects of dietary Lactobicillus plantarum on the growth, carcass quality, and immune response of African catfish Clarias gariepinus Challenged with Salmonella typhi. Journal of Applied Aquaculture, 29 (1): 62 – 80

Ogunshe, A.O, P. O. Olabode. 2009. Antimicrobial potentials of indigenous Lactobacillus strains on gram-negative indicator bacterial species from Clarias gariepinus (Burchell.): microbial inhibition of fish-borne pathogens. African Journal of Microbiology Research, 3(2): 870 – 876.

Osuigwe, D. I., C. Nwosu, J. O. Ogunji. 2007. Preliminary observations on some haematological parameters of juvenile Heterobranchus longifilis fed different dietary levels of raw and boiled jackbean (Canavalia ensiformis) Seed Meal. Tropentag University of Kassel-Witzenhausen and University of Göttingen, October 9 - 11, 2007 Conference on International Agricultural Research for Development, 1-6.

Panigrahi, A., V. Kiron, T. Kobayashi, J. Puangkaew, S. Satoh, H. Sugita. 2004 Immune responses in rainbow trout Oncorhynchus mykiss induced by a potential probiotic bacteria Lactobacillus rhamnosus JCM 1136. Veterinary Immunology and Immunopathology, 102: 379–388.

Pieters, N., J. Brunt, B. Austin, A. R. Lyndon. 2008.. Efficacy of in-feed probiotics against Aeromonas bestiarum and Ichthyophthirius multifiliis skin infections in rainbow trout (Oncorhynchus mykiss, Walbaum). Journal of Applied Microbiology, 105: 723-732.

Pineiro M., C. Stanton. 2007. Probiotic bacteria: legislative framework - requirements to evidence basis. Journal of Nutrition, 137: 850 - 853.

Ranzani-Paiva, M. J. T., C. M. Ishikawa, A. A das Eiras, N. N. Felizardo. 2000. Haemotological analysis of ‘chara’ Pseudoplatystoma fasciatum in captivity. In: Aqua 2000: Responsible Aquaculture in the New Millennium, Nice, France, 2–6 May 2000. Special publication 28. European Aquaculture Society, 590pp.

Ranzani-Paiva, M. J. T., E. L. Rodrigues, M. L. Veiga, A. C. Eiras, B. E. S. Campos. 2003. Differential Leukocytes Counts in “Dourado” Salminus maxillous Valenciennes, 1840, from the Mogi-Guacu River, Pirassiununga, SP. Brazilian Journal of. Biology, 63: 517-525.

Shah, B., N. Burg, M. H. Pillinger. 2017. Neutrophils. In: Kelley and Firestein's Textbook of Rheumatology (Tenth Edition) Volume 1, 2017, pp.169-188.

Siezen, R. J., J. E. T. van Hylckama Vlieg. 2011. Genomic diversity and versatility of Lactobacillus plantarum, a natural metabolic engineer. Microbial Cell Factories, 10 (1): (S3).

Sinyakov, M. S., M. Dror, H, M. Zhevelev, S. Margel, R. R. Avtalion. 2002. Natural antibodies and their significance in active immunization and protection against a defined pathogen in fish. Vaccination. 20: 3668-3674.

Svobodova, Z., D. Pravda, J. Palackova. 1991. Unified methods of haematological examination of fish. Research Institute of Fish Culture and Hydrobiology, Vodnany, Czech Republic, 31 pp.

Zhang, Q, A. Ding, Q. Yue, W. Li, Y. Zu, 2017. Dynamic interaction of neutrophils and RFP-labelled Vibrio parahaemolyticus in zebrafish (Danio rerio). Aquaculture and Fisheries, 2 (6): 269 – 277.

Zhou X., M. Li, K. Abbas. 2009. Comparison of haematology and serum biochemistry of cultured and wild Dojo loach Misgurnus anguillicaudatus. Fish Physiology and Biochemistry, 35:435–441.




DOI: https://doi.org/10.13170/ajas.7.3.24925

Article Metrics

Abstract view : 0 times
PDF - 0 times

Refbacks

  • There are currently no refbacks.


DETAIL VISITORS STATISTIC COUNTER CLICK HERE

 

This work is licensed under a Creative Commons Attribution - 4.0 International Public  License (CC - BY  4.0).