Impacts of combination of onion bulb, holy basil and turmeric rhizome on shelf- life, flesh and microbial quality of smoked Clarias gariepinus

Sunday Emmanuel Olusola, Toyosi Elizabeth Martins


The increasing use of synthetic antimicrobials has created a situation leading to a pond ecological imbalance and enrichment of multiple multi-resistant pathogenic microorganisms. This study was conducted to investigate the impact of spices and natural preservatives using a mixture of Onion Bulb (OB), Holy Basil (HB) and Turmeric Rhizome (TR) on shelf–life, flesh and microbial quality of smoked Clarias gariepinus during 56 days storage. Spices were added at the point of processing with five treatments of 8 fresh C. gariepinus (1.2-2kg) were distributed into experimental containers; the control (without Onion Bulb, Holy Basil and Turmeric Rhizome), (TR + OB)2, (TR+ HB)3, (OB +HB)4, (TR + OB + HB)5 and the experiment were carried out in triplicates. Clarias gariepinus were smoked in a smoking kiln at 40ºC- 60ºC and 120-150ºC for 6 and 18 hours respectively. Proximate composition, biochemical parameters, organoleptic assessment, and microbial analysis were carried out using standard methods. The result showed that the crude protein of C. gariepinus was higher in the treated groups compared to the control. Also, the result shows that the biochemical parameters, organoleptic assessment and the microbial loads in smoked C. gariepinus were reduced in the treated groups than in the control at 1 day, 28 days and 56 days storage respectively. The results indicated that using onion bulb, holy basil, and turmeric rhizome in combination may be useful in improving the shelf life, consumer acceptability of smoked C. gariepinus and preventing bacteria pathogens in smoked C. gariepinus.

Keywords: Catfish, Onion Bulb, Holy Basil, Microbial Loads, Turmeric Rhizome

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Abdul, M.S., M. Aktar. 2011. Changes of nutritional value of three marine dry fishes (Johniusdus sumieri, Harpodon nehereus and Lepturacanthus savala) during storage. Food and Nutrition Sciences, 2: 1082-1087.

Adeniyi, O.V., F.E. Olaifa, B.O. Emikpe, S.T. Ogunbanwo. 2021. Effects of Tamarindus indica (Linnaeus 1753) pulp-fortified diets on the gut microflora and morphometry in African catfish Clarias gariepinus (Burchell 1822). Aceh Journal of Animal Science, 6 (2): 45 – 51.

Akter, T., A.T.A. Ahmed., M.A. Khaleque, M. Begum. 2013. Effect of drying on quality of Tengra (Mystus vittatus) treated with turmeric and salt. Unique Research Journal of Biological Science, 1: 1-5.

Alex,T. ,K. Eagappan. 2017. An empirical study on the effect of spices and herbs in the shelf life of dried Indian achory fish. IOSR Journal of Agriculture and Veterinary Science, 10 (3):1-7.

Amuneke K.E., O. R. Oguntade, F.C. Ikeogu, U.A. Nomeh. 2020. Effect of natural preservatives on the organoleptic characteristics and storage stability of smoked Heterotis Niloticus. Agro-Science, 19 (2), 31-35. DOI:

Association of Official Analytical Chemist, AOAC 2005. Official methods of analysis (W. Horwit zeachtor) 18th edition A.O.A.C Washington, DC: 129-146.

Ayoola, S.O., A.A. Bamiro. 2017. Genotoxicity, haematological and growth performance of the African catfish Clarias gariepinus fingerlings fed walnut Tetracarpidium conophorum leaves to substitute for rice bran. Aceh Journal of Animal Science, 2(2): 64-76.

Bello, O. S. 2014. Performance and antimicrobial potentials of onion (Allium cepa Linn) bulb and walnut (Tetracarpidium conophorum Mull. Arg) leaves in the diet of Clarias gariepinus. Ph.D Thesis, University of Ibadan, Ibadan, Nigeria. 187pp.

Bello, O.S, F.E. Olaifa, B.O. Emikpe, S.T. Ogunbanwo. 2013. Potentials of walnut (Tetracarpidium conophorum) leaf and onion (Allium cepa) bulb extracts as antimicrobial agents for fish. African Journal of Microbiology Research, 7 (19): 2027 – 2033. Doi: 10.5897/AJMR12.814

Bober, Z., S. Agnieszka, A., David, O. Lukasz, B. Dovota. 2018. Review on medicinal benefits from the use of black pepper, curcuma and ginger. European Journal of Clinical and Eperimental Medicine, 16 92):133 - 145

Campos,T., S. Barreto, R. Queiros, S. Ricardo-Rodrigues, M.R. Felix, M. Laurayo 2015. Conservacao de morangos com utiliacao de oleos essenciais, AGROTEC, 18:90 -96.

Ekelemu, J.K., A.A. Nwabueze, A.E. Irabor, N.J. Otuye. 2021. Spicing: A means of improving organoleptic quality and shelf life of smoked catfish, Scientific African, 13: 1-7.

Embuscado, M.E. 2015. Spices and herbs: Natural sources of antioxidants – a mini review, Journal of Functional Foods, 18: 811–819.

Farid, F.B, G.A. Latifa, M.N. Nahid, M. Begum. 2014. Effect of sun-drying on proximate composition and pH of Shoal fish (C. striatus; Bloch, 1801) treated with Salt and Salt-turmeric storage at Room Temperature (270 - 300C). IOSR Journal of Agriculture and Veterinary, 7(9): 1-8.

Hassan, M.N. M. Rahman, M.M. Hossain, A.A.K.M. Nowsad, M.B. Hossain. 2013. Post-Harvest Loss and Shelf Life of Traditionally Smoked Shrimp Products Produced in Bangladesh. World J. of Fish and Marine Sci. 5 (1): 14-19.

Isaac, A.O, S.E. Olusola, O.A. Oyelese. 2014. Microbial safety and quality of smoked Clarias gariepinus Burchell, 1822 on different concentrations of preservatives during ambient storage. International Journal of Aquaculture, 4 (21): 123 – 130. doi: 10.5376/ija.2014.04.0021

Kiin-Kabari, D.B, I.S. Barimalaa, S.C. Achinewhu, T.A. Adeniji. 2011. Effects of extracts from three indigenous spices on the chemical stability of smoke-dried catfish (Clarias lezera) during storage. African Journal of Food, Agriculture, Nutrition and Development, 11(6): 5335 – 5343.

Kumolu-Johnson C.A., P.E. Ndimele, A.O. Ayorinde T.I. Ojikutu. 2015. Preliminary study on the antioxidative and anti-fungal effects of ginger oil on the shelf life of hot smoked fish. American Journal of Food Technology, 10: 74-78.

Marimuthu, K., H. Palaniandy, Z. A. Muchlisin. 2019. Effect of different water pH on hatching and survival rates of African catfish Clarias gariepinus (Pisces: Clariidae). Aceh Journal of Animal Science, 4(2): 80-88.

Olusola, S.E. 2021. Influence of smoking and natural preservatives on shelf – life and microbial quality of Clarias gariepinus during storage, Ife Journal of Science, 23(1): 145-151.

Taniya, A., E. Kannan. 2016. Effect of Spices and Herb for Enhancing Microbial Quality and Shelf Life of Dried Indian Oil Sardine (sardinella longiceps) Fish during Storage at Room Temperature, IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT), 10 (1): 44-51.

Wakoli, A.B., D.A.O. Onyango, P.J. Rotich. 2014. Effect of selected spices on food spoilage rate. Global Journal of Biology, Agriculture and Health Sciences, 3(4): 160-162.

Yoganathan, T. 2020. Effects of turmeric on quality attributes of brine salted dried Altantic Pollock (Pollachious virens) UNESCO GRO Fisheries Training Programme, Iceland, final project, 47pp



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