Kemampuan Daya Hambat Beberapa Macam Sabun Antiseptik Terhadap Pertumbuhan Staphylococcus aureus dan Escherichia coli

Abstract

This research is  aimed to find out ability  some kind antiseptic soaps inhibiting the growth of Staphylococcus aureus and Eschericia coli bacteria. The research method used Randomized Completely Block Design Sub Sampling with 6 treament and 3 replication, they are: antiseptic soap A, B, C, D, phenol 2% and aquadest. The group consist of S. aureus and E. coli bacteria. The inhibit experiment was done by using Kirby-Bauer methode. The parameter analyzed by using Analysis of Variance (ANOVA) continue with the Duncan’s Multiple Range Test (DMRT). The result showed that there were  significant differences of inhibit zone that formed from both bacterias . The result of the antiseptic soap suspend inhibit zone, S. aureus bacteria has larger diameter than E. coli. Soap A, B, C and D with 50% concentrate toward both bacteria, each has average diameter 25, 16 mm, 15,6 mm, 11,5 mm and 9,83 mm while phenol  has 15,0 mm. This result showed that soap A, B, and C has the equal ability to phenol 2%. Base on Duncan Multiple Range Test (DMRT), it is found out that the real differences of the inhibit zone between the two bacteria as well the real differences of treatment forward those bacteria (P<0,05).