Nurul Isnaini, Tri Harsi, Devi Maharani


This study was carried out to evaluate the seasonal variation on semen characteristics of Murrah buffalo bulls in Indonesia. A total of three Murrah buffalo bulls were included in this study. The semen was collected once a week during the rainy season (from January to April and from October to December) and the dry season (from May to September). Immediately after collection, the semen volume, sperm concentration, total sperm output, individual motility, post-thawing motility, and straw production were evaluated. The results showed that the season did not change (P>0.05) the semen volume, individual motility, and post-thawing motility. However, sperm concentration, total sperm output, and straw production were decreased in the dry season (P<0.05) as compared to the rainy season. In conclusion, several semen characteristics of Murrah buffalo bulls may vary depending on the season of collection. The semen collection during the rainy season is more profitable than the dry season.


artificial insemination; Bubalus bubalis; dry season; rainy season; semen cryopreservation

Full Text:



Abdolghafour, B. and A. Saghir. 2014. Buffalo: a potential animal for quality meat production-A review. Livest. Res. Int. 2: 19-29.

Bhakat, M., T.K. Mohanty, A.K. Gupta, S. Prasad, A.K. Chakravarty and H.M. Khan. 2015. Effect of season on semen quality parameters in Murrah buffalo bulls. Buffalo Bull. 34: 100-112.

Cárdenas‐Gallegos, M.A., J.R. Aké‐López, F. Centurión‐Castro and J.G. Magaña‐Monforte. 2012. The breed and season effects on scrotal circumference and semen characteristics of hair sheep rams under tropical conditions. Reprod. Domest. Anim. 47: e92-e94.

Directorate General of Livestock and Animal Health. 2018. Livestock and Animal Health Statistics. Ministry of Agriculture, Jakarta, Indonesia.

Golher, D.M., A. Kumaresan, K.K. Saraf, S. Chhillar, S. Nayak, U.K. Tripathi, C.N. Bhaskar, S.S. Lathwal and T.K. Mohanty. 2018. Influence of season and climatic variables on testicular cytology, semen quality and melatonin concentrations in crossbred bucks reared under subtropical climate. Int. J. Biometeorol. 62: 1709-1719.

Hoque, M.R., M.S. Rana, S.B. Nayan, M.F.H. Miraz, G.K. Deb, T.N. Nahar, R. Habib and M.S.R. Siddiki. 2018. Influence of multiple showering on quality of buffalo semen during hot-humid season. J. Adv. Vet. Anim. Res. 5: 12-18.

Isnaini, N., S. Wahjuningsih and E. Adhitama. 2019. Seasonal effects on semen quality of Ongole crossbred and Simmental bulls used for artificial insemination. Livest. Res. Rural Dev. 31: #Article 16.

Koonjaenak, S., V. Chanatinart, S. Aiumlamai, T. Pinyopumimintr and H. Rodriguez‐Martinez. 2007. Seasonal variation in semen quality of swamp buffalo bulls (Bubalus bubalis) in Thailand. Asian J. Androl. 9: 92-101.

Losano, J.D., D.S. Angrimani, A. Dalmazzo, C.C. Rocha, M.M. Brito, E.G. Perez, R.H. Tsunoda, P.A. Góes, C.M. Mendes, M.E. Assumpção and V.H. Barnabe. 2018. Effect of vitamin E and polyunsaturated fatty acids on cryopreserved sperm quality in Bos taurus bulls under testicular heat stress. Anim. Biotechnol. 29: 100-109.

Marai, I.F.M. and A.A.M. Haeeb. 2010. Buffalo's biological functions as affected by heat stress—A review. Livest. Sci. 127: 89-109.

Olurode, S.A., V.O. Sekoni, I.O. Oyenekan and A.S. Adetomiwa. 2018. Effect of season on reproduction in west African Dwarf Bucks. Niger. Vet. J. 39: 35-44.

Silva, L.K.X., J.S. Sousa, A.O.A. Silva, J.B. Lourenço Junior, C. Faturi, L.G. Martorano, I.M. Franco, M.H.A. Pantoja, D.V. Barros and A.R. Garcia. 2018. Testicular thermoregulation, scrotal surface temperature patterns and semen quality of water buffalo bulls reared in a tropical climate. Andrologia. 50: e12836.

Soren, S., S.V. Singh and P. Singh. 2016. Influence of season on seminal antioxidant enzymes in Karan Fries bulls under tropical climatic conditions. Turk. J. Vet. Anim. Sci. 40: 797-802.

Van Tilburg M.F., M.G. Salles, M.M. Silva, R.A. Moreira, F.B. Moreno, A.C. Monteiro-Moreira, J.A. Martins, M.J. Cândido, A.A. Araújo and A.A. Moura. 2015. Semen variables and sperm membrane protein profile of Saanen bucks (Capra hircus) in dry and rainy seasons of the northeastern Brazil (3° S). Int. J. Biometeorol. 59: 561-573.


Article Metrics

Abstract view : 0 times
PDF - 0 times


  • There are currently no refbacks.

Indexed by:


p-ISSN: 1978-225X e-ISSN: 2502-5600 Copyright© 2007-2021