HISTOMORPHOLOGY OF PANCREATIC TISSUE IN MEDAKA FISH (Oryzias javanicus): STUDY OF DIABETIC ANIMAL MODEL DEVELOPMENT

Muhammad Ichlasul Akmal, Sri Rahmatul Laila, Tutik Wresdiyati

Abstract


This study aimed to explore the histomorphology of the pancreatic tissue of medaka fish to develop medaka fish as a diabetic animal model. Adult medaka fish were obtained with a body length of 2.53±0.22 cm and a weight of 265.8±25 g. Medaka fish were fixed using Boiun's fixative solution. Macro anatomical observations of the pancreas were conducted using a stereomicroscope. Histological preparations were made according to the Hematoxylin-eosin staining standard procedure. Pancreatic histomorphology of medaka fish was carried out using a light microscope with 100x and 400x magnification. The macro anatomy observations of the pancreas showed the interconnection with several tissues, such as spleen, gallbladder, liver, fat, and intestines. The pancreas was located toward the liver, oversees the intestines, and covered with fat. Pancreatic histomorphological observations showed exocrine cells composed of serous pyramidal acinar cells and endocrine cells (islets of Langerhans). Intralobular and intercalated ducts (ducts of Boll) were composed of simple cuboidal epithelium. This study concluded that the pancreas organ in medaka fish is a single organ, and histomorphologically has both of exocrine and endocrine cells.


Keywords


histomorphology; langerhans; medaka fish; oryzias; pancreas

Full Text:

PDF

References


Benchoula, K., Khatib, A., Quzwain, F. M. C., Che Mohamad, C. A., Wan Sulaiman, W. M. A., Wahab, R. A., Ahmed, Q. U., Ghaffar, M. A., Saiman, M. Z., Alajmi, M. F., dan El-Seedi, H. (2019). Optimization of hyperglycemic induction in zebrafish and evaluation of its blood glucose level and metabolite fingerprint treated with psychotria malayana Jack Leaf extract. Molecules. 24(8): 1506

Biemar, F., Argenton, F., Schmidtke, R., Epperlein, S., Peers, B., dan Driever, W. (2001). Pancreas development in zebrafish: Early dispersed appearance of endocrine hormone expressing cells and their convergence to form the definitive islet. Developmental Biology. 230(2): 189-203

Chen, S., Li, C., Yuan, G., dan Xie, F. (2007). Anatomical and histological observation on the pancreas in adult zebrafish. Pancreas. 34(1): 120-5

Furutani-Seiki, M., dan Wittbrodt, J. (2004). Medaka and zebrafish, an evolutionary twin study. Mechanisms of Development. 121(7): 629–637.

Greek, C. R., dan Greek, J. S. (2003). Sacred Cows and Golden Geese: The Human Cost of Experiments on Animals. Bloomsbury Academic, New York

Hall, J. E., dan Hall, M. E. (2021). Guyton and Hall: Textbook of Hall, J. E., dan Hall, M. E. (2021). Guyton and Hall: Textbook of Medical Physiology 14th Edition. Elsevier, 973–989..

Handajani, A., Roosihermiatie, B., dan Maryani, H. (2012). Faktor-Faktor Yang Berhubungan Dengan Pola Kematian Pada Penyakit Degeneratif Di Indonesia. Buletin Penelitian Sistem Kesehatan, 13(1).

International Diabetes Federation. (2021). IDF Diabetes Atlas|Tenth Edition (Tenth Edit). International Diabetes Federation.

Kesuma Sari, D., Andriani, I., dan Yaqin, K. (2018). Micromorphological Observation of the Anterior Gut of Sulawesi Medaka Fish (Oryzias celebensis). International Journal of Current Microbiology and Applied Sciences, 7(2), 2942–2946.

Khazaee, M., dan Ng, C. A. (2018). Evaluating parameter availability for physiologically based pharmacokinetic (PBPK) modeling of perfluorooctanoic acid (PFOA) in zebrafish. Environmental Science: Processes and Impacts. 20(1):105-119

Kiernan, J. (2008). Histological and Histochemical Methods: Theory and Practice, 4th edition. Cold Spring Harbor Laboratory Press.

Lawrence, C., Adatto, I., Best, J., James, A., dan Maloney, K. (2012). Generation time of zebrafish (Danio rerio) and medakas (Oryzias latipes) housed in the same aquaculture facility. Lab Animal. 41(2012): 158-165

Magtoon, W., dan Termvidchakorn, A. (2009). A Revised Taxonomic Account of Ricefish Oryzias ( Beloniformes ; Adrianichthyidae ), in Thailand , Indonesia and Japan. Fisheries Research, 9(1): 35–68.

Menke, A. L., Spitsbergen, J. M., Wolterbeek, A. P. M., dan Woutersen, R. A. (2011). Normal anatomy and histology of the adult zebrafish. Toxicologic Pathology, 39(5): 759–775.

Palazón-Fernández, J. L., Peiro Suso, M., Miguel Mancera, J., dan Sarasquete, C. (2011). Immunohistochemical study of the principal pancreatic islet of the toadfish, Halobatrachus didactylus (Pisces: Batrachoididae). Acta Histochemica. 113(3):256-61

Pan, H., Guo, R., Zhu, J., Wang, Q., Ju, Y., Xie, Y., Zheng, Y., Wang, Z., Li, T., Liu, Z., Lu, L., Li, F., Tong, B., Xiao, L., Xu, X., Li, R., Yuan, Z., Yang, H., Wang, J., … Liu, L. (2018). A gene catalogue of the Sprague-Dawley rat gut metagenome. GigaScience. 7(5): 055

Qadori, Y. T. (2011). Histological Studies on Pancreatic Tissue in Diabetic Rats by Using Wild Cherry. The Iraqi Postgraduate Medical Journal. 10(3): 421-425

Rahardjo, M. F., Affandi, R., Sjafei, D. S., Sulistiono, dan Hutabarat, J. (2011). Iktiology (p. 395).

Sadat, A., dan Nurunnabi, M. (2012). Animal Research in Medical Science: Pros and Cons. The Journal of Popular Medical College. 2(1): 28-33

Taylor, K., dan Alvarez, L. R. (2019). An Estimate of the Number of Animals Used for Scientific Purposes Worldwide in 2015. Alternatives to Laboratory Animals : ATLA. 47(5-6):196-213

The Institutional Animal Care and Use Committee. (2018). Guide For The Care and Use of Laboratory Animals (J. C. Garber, R. W. Barbee, J. T. Bielitzki, L. A. Clayton, J. C. Donovan, C. F. M. Hendriksen, dan D. F. Kohn (eds.); 8th ed.). The National Academies Press.

Wang, X., Yang, X. liang, Liu, K. chun, Sheng, W. long, Xia, Q., Wang, R. chun, Chen, X. qiang, dan Zhang, Y. (2020). Effects of streptozotocin on pancreatic islet β-cell apoptosis and glucose metabolism in zebrafish larvae. Fish Physiology and Biochemistry. 46(3):1025-1038

Zhu, T., Gui, L., Zhu, Y., Li, Y., dan Li, M. (2018). Dnd is required for primordial germ cell specification in Oryzias celebensis. Gene, 679(August), 36–43.




DOI: https://doi.org/10.21157/j.ked.hewan.v17i1.26884

Article Metrics

Abstract view : 0 times
PDF - 0 times

Refbacks

  • There are currently no refbacks.


Copyright (c) 2023 by author and J. Kedokt. Hewan

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indexed by:

           
  

p-ISSN: 1978-225X e-ISSN: 2502-5600 Copyright© 2007-2021