RELIABILITY OF AMYLOID DETECTION IMMUNOASSAY KITS FOR ALZHEIMER’S DISEASE SCREENING: A PRELIMINARY STUDY

Indah Aprianti Putri, Emilna Mega Ningrum, Rachmitasari Noviana, Silmi Mariya, Uus Saepuloh, Elok Budi Retnani, Muhammad Hamdan, Jusak Nugraha, Huda Shalahudin Darusman

Abstract


Alzheimer’s Disease is a progressive neurodegenerative disease that damages the brain and is part of dementia. Alzheimer’s dementia is caused by various factors that until now the proper therapy is uncertain. Kit production as a diagnostic tool to support the development of screening for Alzheimer’s disease is a strategic effort. Screening based on detection with Enzyme-Linked Immunosorbent Assay (ELISA) can work effectively and accurately to optimize a specific antibody material or monoclonal antibody to the Aβ42 peptide. This study aimed to research the reliability of the amyloid detection kit which was developed as an alternative screening test for Alzheimer’s disease compared to commercially available kits. Based on the results and interpretation of  inter and intra-assay coefficients, the in-house ELISA kit has comparable Aβ42 detection to the commercially available ELISA kit

Keywords


Aβ42, Alzheimer’s disease, ELISA, screening, reliability kit

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DOI: https://doi.org/10.21157/j.ked.hewan.v18i3.31109

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