The Effect of Vitamin E Supplementation on the Histopathological Changes of The Hearts Mice Given High Fat Diet

Abstract

The aim of this study was to determine the protective effect of vitamin E on mice heart. Twentyfive
male
mice
weighing
±
40
g
at
the
age
of
3
months
were
used.
The
mice
were
adapted
for
a
week
and

fed
basal
diet.
The

mice
were
fed
normal
diet
as
control
(group
1),
high
fat
+
50
IU
Vitamin
E
(group
II);

high

fat + 100 IU Vitamin E (group III) high fat + 200 IU Vitamin E (group IV); high fat + 400 IU
Vitamin E (Group V). Diet was given about 10% body weight, water was given ad libitum every day.
After 3 months, all animal were then killed. The hearts were collected for routine histopathological
examination. The result indicate that lesions in the heart consist of swollen eosinophilic hyalinization of
cytoplasm, vacuolization, and necrosis myocardium (cardiomiopathy) in all mice receiving high fat diet
with less than 400 IU Vitamin E.

Keywords: high fat diet, vitamin E, cardiovascular diseases