DETECTION OF MULTIDRUG-RESISTANT Klebsiella pneumoniae IN THE UPPER RESPIRATORY TRACT OF BROILERS AND LAYER CHICKENS FROM COMMUNITY FARM IN ACEH BESAR, INDONESIA

Abstract

This study aimed to investigate the presence of multidrug-resistant (MDR) Klebsiella pneumoniae in upper respiratory tract of broiler chickens
and laying hens from community farms in Aceh Besar. Tracheal swab samples were collected and cultured on eosin methylene blue agar for bacterial isolation. Suspected isolates were identified through biochemical testing and confirmed via PCR targeting the rpoB gene. Identified isolates were tested for antibiotic susceptibility against ciprofloxacin, tetracycline, gentamicin, erythromycin, and chloramphenicol, using the disc diffusion
method. The results revealed K. pneumoniae in 6% of broiler and 14% of laying hens, with widespread multidrug resistance. All isolates were
resistant to erythromycin (100%), gentamicin (100%), tetracycline (85%), ciprofloxacin (40%), and chloramphenicol (15%). These findings
concluded that there has been the spread of MDR of K. pneumoniae in broiler chickens and laying hens within Aceh Besar’s community farms in
Aceh Besar, Indonesia.