Cytoskeleton of Bovine Oocytes after Vitrification Using Ethylene Glycol Cryoprotectant

Abstract

The objective of this research is to know the influence of vitrification method toward the
condition of bovine oocytes cytoskeleton based on microtubules using the immunohistochemistry
technique. Oocytes classified in two treatments such as fresh oocytes (control) and oocytes with
vitrification treatments of 10, 20, 30, 40, and 50% of ethylene glycol (EG). Data were analyzed by
descriptive analyzing. The results of this research indicated that vitrification treatments were affected to
microtubules organization of oocytes. Concentration of EG 10, 20, 40, and 50% of EG could not repair
cytoskeleton after vitrification, but cytoskeleton structure could be protected by EG 30%.