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Perbandingan kecepatan pertumbuhan Escherichia coli non ESBL dengan Escherichia coli ESBL

Branandito Putra, Linda Dewanti, Eddy Bagus Wasito

Abstract


Abstrak. Penelitian ini bertujuan untuk mengidentifikasi perbedaan kecepatan pertumbuhan antara kelompok bakteri Escherichia coli NON ESBL dengan kelompok Escherichia coli ESBL. Jenis dan rancangan penelitian ini adalah penelitian eksperimental. Besar sampel sebanyak 13 isolat bakteri Escherihcia coli. Teknik sampling yang digunakan non random sampling. Variabel dalam penelitian ini meliputi Isolat Escherichia coli, media cair, fotometer, suhu inkubasi. Kecepatan pertumbuhan bakteri Escherichia coli NON ESBL, yaitu 2,206 ± 0,789. Sedangkan kecepatan pertumbuhan bakteri Escherichia coli ESBL, yaitu 1,963 ± 0,674. Nilai signifikasi Uji T sampel independen perbandingan kecepatan pertumbuhan bakteri Escherichia coli NON ESBL dengan kecepatan pertumbuhan bakteri Escherichia coli ESBL (p = 0,257) yang artinya tidak ada perbedaan yang signifikan.Hal tersebut terjadi karena jumlah sampel penelitian yang kurang dari jumlah minimal sampel yang diperlukan.

Kata kunci: Escherichia coli, ESBL, Pertumbuhan.

Abstract. This study aims to identify a difference of growth’s velocity between NON ESBL Escherichia coli with ESBL Escherichia coli. Type and design of this study was experimental. The sample size was 13 isolate of Escherichia coli. Sampling technique used was non random sampling. Variables in this study were Isolate of Escherichia coli, liquid media, photometer, temperature of incubation. The velocity of growth in NON ESBL Escherichia coli, was 2,206 ± 0,789. Meanwhile, The velocity of growth in ESBL Escherichia coli, was 1,963 ± 0,674. Significant value of T independent sample test  comparison of growth velocity between NON ESBL Escherichia coli  with ESBL Escherichia coli (p =  0,257) which means there’s no different significant. .It happens because an amount of total sample less than minimum standards amount of sample.

 

Keywords: Escherichia coli, ESBL, Growth.


Keywords


Kata kunci: Escherichia coli, ESBL, Pertumbuhan; Escherichia coli, ESBL, Growth

References


Daftar pustaka

Andersson DI, Hughes D. 2010. Antibiotic Resistance and It’s Cost : Is It Possible to Reverse Resistance?,Vol.8. Nature Review Microbiology. Uppsala: Macmillan Publishers Ltd., pp260-271.

Bradford, P.A. (2001). Extended spectrum beta-lactamases in the 21st century: characterization, epidemiology and the detection of this important resistance threat. Clin Microbiol Rev,14,933-951.

Imaniah, B.A., 2015. Peta Kuman dan Resistensinya terhadap Antibiotika pada Penderita Infeksi Saluran Kemih (ISK) di RSUD Dr. Moewardi Tahun 2014, Skripsi, Fakultas Farmasi Universitas Muhammadiyah Surakarta.

Jawetz, E., Melnick, J. L., & Adelberg, E. A., 2001, Medical Microbiology, Twenty Second ED., diterjemahkan oleh Bagian Mikrobiologi Fakultas Kedokteran Airlangga,224-225,317-349, 352, Jakarta, Salemba Medika.

Kementerian Kesehatan Republik Indonesia, 2015. Pedoman Program Pengendalian Resistensi Antimikroba di Rumah Sakit. Direktorat Jenderal Bina Kefarmasian dan Alat Kesehatan, Jakarta, hal.10-13.

Madigan M.T. and Martinko J.M., 2005. Brock Biology of Microorganisms 11th ed., Prentice Hall, New Jersey.

Martinez JL, Baquero F. 2002. Interactions among Strategies Associated with Bacterial Infection : Pathogenicity, Epidemicity, and Antibiotic Resistance.Clin. Microbiol, 15(4):647.

Redway K., 2012, Virulence and Pathogenicity. (Online)

Samirah, Darwati, Windarwati, and Hardioeno, 2006, Pola dan Sensitivitas Kuman di Penderita Infeksi Saluran Kemih, Indonesian Journal of Clinical Pathology and Medical Laboratory,12 (8),111.

Purnomo, B.B.,2011. Dasar-dasar Urologi, CV Sagung Seto, Jakarta.

Paterson, D.L, and Bonomo, R.A. (2005). Extended-spectrum beta-lactamases:a clinical update. Clin Microbiol Rev,18,657-686.


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DOI: 10.24815/jks.v20i2.18498

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