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  2. Vol 22, No 4 (2022)
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Volume minimum saliva untuk deteksi Human Beta Globin sebagai penanda DNA Genom Manusia

Zulham Yamamoto, Abidah Nur, Tetty Aman Nasution

Abstract


Saliva can be a source of DNA since it contains human cells from the oral mucosa. In addition, saliva contains a variety of normal flora. Recently, saliva has become important as a diagnostic fluid. Saliva is an easily accessible fluid that is similar to blood in many aspects, and can be collected non-invasively. The presence of normal flora, including genomic DNA from microorganisms and food, may interfere the use of saliva as a source of DNA for molecular biology approach of the human genome. The purpose of this study was to determine the minimum volume of saliva for sampling and detection of human genomic DNA. This is an experimental study with a cross-sectional design. A total of 10 subjects were involved in this study. Unstimulated saliva was collected in a saliva pot by spitting. DNA from fresh saliva was extracted using spin column method. PCR of the human beta globin (HBB) gene from the 0.5, 1, 2, and 5-mL saliva volume groups was performed. The amplicons of the HBB gene (335 bp) were found in all saliva volume groups. Conclusion: The minimum volume of saliva required for detection of the human genome is 0.5 mL.

Keywords


beta-globin, biomarker, genome, minimum volume, saliva

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DOI: 10.24815/jks.v22i4.23386

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