THREE-WAY STOP COCKS AS BREATHING CIRCUIT FOR ANESTHETIC PROCEDURES OF ANIMAL STUDY ON WISTAR RATS
Abstract
General anesthesia procedures used in experimental animals are not limited to research in the field of anesthesia. Ventilators and breathing circuit systems for research involving anesthesia procedures in rats are limited in Indonesia. The limitations of ventilators and breathing circuit systems are one of the reasons for the lack of complex and advanced animal experimental studies in Indonesia. Therefore, the authors would like to discuss a general anesthesia procedure for intubation in rats using tools and materials that are readily available in the clinical setting.
An endotracheal tube insertion procedure can use a Miller size 0 laryngoscope. The endotracheal tube uses a 16 G intravenous cannula in which the needle is replaced with a small wire. The 3-way stop cock system can be used as a replacement for the Mapleson E system for the breathing circuit system. The fresh gas flow (FGF) source will be connected to the angled-port, while the other two ports will be connected to the reservoir and the intravenous cannula to the experimental animal. We use FGF 3-5 times as much as minute ventilation and use of a reservoir capacity similar to tidal volume for spontaneous ventilation. Therefore, the oxygen flow rate is set to about 1-1.5 L / min. Reservoir is not required for controlled ventilation.
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References
Bhindi R, Witting PK, McMahon AC, Khachigian LM, Lowe HC. Rat models of myocardial infarction. Pathogenetic insights and clinical relevance. Thromb Haemost. 2006;96(5):602-610. DOI: 10.1160/TH05-07-0514
Snow SJ, McGee MA, Henriquez A, et al. Respiratory Effects and Systemic Stress Response Following Acute Acrolein Inhalation in Rats. Toxicol Sci. 2017;158(2):454-464. DOI: 10.1093/toxsci/kfx108
Whitehead G, Kimmel E, Reboulet J, Still K. Pulmonary Function in Normal Rats. Naval Health Research Center Detachment (Toxicology); 1999. Accessed August 10, 2020. https://www.researchgate.net/publication/235155283_Pulmonary_Function_in_Normal_Rats
Breuer T, Bleilevens C, Rossaint R, et al. Dexmedetomidine Impairs Diaphragm Function and Increases Oxidative Stress but Does Not Aggravate Diaphragmatic Atrophy in Mechanically Ventilated Rats. Anesthesiology. 2018;128(4):784-795. DOI: 10.1097/ALN.0000000000002081
Proctor E, Fernando AR. Oro-endotracheal intubation in the rat. Br J Anaesth. 1973;45(2):139-142. DOI: 10.1093/bja/45.2.139
Yu G, Segel I, Zhang Z, Hogan QH, Pan B. Dorsal Root Ganglion Stimulation Alleviates Pain-related Behaviors in Rats with Nerve Injury and Osteoarthritis. Anesthesiology. 2020;133(2):408-425. DOI: 10.1097/ALN.0000000000003348
Maia L de A, Samary CS, Oliveira MV, et al. Impact of Different Ventilation Strategies on Driving Pressure, Mechanical Power, and Biological Markers During Open Abdominal Surgery in Rats. Anesth Analg. 2017;125(4):1364-1374. DOI: 10.3389/fphys.2019.01513
Rivard AL, Simura KJ, Mohammed S, et al. Rat intubation and ventilation for surgical research. J Investig Surg Off J Acad Surg Res. 2006;19(4):267-274. DOI:10.1080/08941930600778297
Stark RA, Nahrwold ML, Cohen PJ. Blind oral tracheal intubation of rats. J Appl Physiol. 1981;51(5):1355-1356. DOI: 10.1152/jappl.1981.51.5.1355
Schaefer CF, Brackett DJ, Downs P, Tompkins P, Wilson MF. Laryngoscopic endotracheal intubation of rats for inhalation anesthesia. J Appl Physiol. 1984;56(2):533-535. DOI: 10.1152/jappl.1984.56.2.533
Kaul TK, Mittal G. Mapleson’s Breathing Systems. Indian J Anaesth. 2013;57(5):507-515. DOI:10.4103/0019-5049.120148
Inkster JS. The T-Piece Technique in Anaesthesia: An Investigation into the Inspired Gas Concentrations. Br J Anaesth. 1956;28(11):512-519. DOI: 10.1093/bja/28.11.512
Harrison GA. Ayre’s T-Piece: A Review of Its Modifications. Br J Anaesth. 1964;36:115-120. DOI: 10.1093/bja/36.2.115
Butterworth J, Wasnick J, Mackey D. Morgan and Mikhail’s Clinical Anesthesiology. 6th ed. New York: McGraw-Hill; 2018. ISBN-13: 9781259834424
Nugroho J, Yuniarti WM, Wardhana A, Ghea C. Modification on acute myocardial infarction model through left anterior descending coronary artery ligation: An experimental study on rats (Rattus norvegicus) using readily available materials. Vet World. 2019;12(9):1448-1453. doi: 10.14202/vetworld.2019.1448-1453
DOI: 10.24815/jks.v22i2.23561
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