THREE-WAY STOP COCKS AS BREATHING CIRCUIT FOR ANESTHETIC PROCEDURES OF ANIMAL STUDY ON WISTAR RATS

Ardyan Wardhana, Johanes Nugroho

Abstract


General anesthesia procedures used in experimental animals are not limited to research in the field of anesthesia. Ventilators and breathing circuit systems for research involving anesthesia procedures in rats are limited in Indonesia. The limitations of ventilators and breathing circuit systems are one of the reasons for the lack of complex and advanced animal experimental studies in Indonesia. Therefore, the authors would like to discuss a general anesthesia procedure for intubation in rats using tools and materials that are readily available in the clinical setting. 

An endotracheal tube insertion procedure can use a Miller size 0 laryngoscope. The endotracheal tube uses a 16 G intravenous cannula in which the needle is replaced with a small wire. The 3-way stop cock system can be used as a replacement for the Mapleson E system for the breathing circuit system. The fresh gas flow (FGF) source will be connected to the angled-port, while the other two ports will be connected to the reservoir and the intravenous cannula to the experimental animal. We use FGF 3-5 times as much as minute ventilation and use of a reservoir capacity similar to tidal volume for spontaneous ventilation. Therefore, the oxygen flow rate is set to about 1-1.5 L / min. Reservoir is not required for controlled ventilation. 


Keywords


anesthesia; animal model research; breathing circuit; rats; research laboratory

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DOI: 10.24815/jks.v22i2.23561

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