Efektivitas pemberian suplemen omega-3 terhadap kadar kolesterol total pada tikus galur wistas (Rattus norvegicus) yang diinduksi aloksan

Abstract

Diabetes mellitus (DM) as a risk factor for hypercholesterolemia which triggers coronary heart disease (CHD) as the second highest cause of death after stroke in Indonesia. This study aims to determine the effectiveness of omega-3 supplement in reducing total cholesterol levels in alloxan-induced Wistar rats. This research method is posttest with control group design. Samples were 30 male rats, aged 2-3 month, weight 150-200 grams. Rats were grouped into 6 groups with (K1) normal control, (K2) high-fat diet and alloxan-induced group, (K3) high-fat diet group, alloxan and simvastatin induction, (P1) high-fat diet group, alloxan induction and omega-3 supplements at a dose 36 mg/kg, (P2) high-fat diet group, alloxan induction and omega-3 supplements at a dose 72 mg/kg, and (P3) high-fat diet group, alloxan induction and omega-3 supplements at a dose 144 mg/kg. Giving omega-3 supplements were given for 7 days orally, then the total blood cholesterol levels were measured using a spectrophotometer from the rats. One Way Anova test showed that there was an effectiveness of omega-3 supplements to reduce total cholesterol levels (p= 0,003). Post-Hoc Least Significance Different (LSD) test results that there was no difference between the positive group and treatment group one (P1) because it had the same effect (p= 0.798), so it could be concluded that the provision of omega-3 supplements at a dose of 36 mg / kgBB is best in reducing total cholesterol levels because omega-3 can affect the mechanism of lipoprotein production in the liver which is secreted in the blood.