The Effect Of Kirinyuh Leaves Aqueous Extract (Chromolaena odorata (L) R.M.King & H.Rob.) on Phase 2 Collagen Density Wound Healing in Mice (Mus musculus)
Abstract
Introduction: Various plants have been extensively researched for their ability to hasten wound healing. Kirinyuh plants (Chromolaena odorata (L) R.M.King & H.Rob.) includes several secondary metabolite chemicals that can help to accelerate wound healing.
Objective: To determine the effect of water extract of kirinyu leaves (C. odorata) on collagen density at the end of phase 2 wound healing in mice (Mus musculus) and the amount of concentration of water extract of kirinyu leaves (C. odorata) that is most effective in collagen formation.
Methods: A total of 28 male mice aged 2-3 months were given an incision wound on the back and separated into four treatment groups by giving kirinyuh leaf aqueous extract (C. odorata). Group I received no therapy (control), Group II received 2.5% extract administration, Group III received 5% extract administration, and Group IV received 7.5% extract administration. Wound healing was observed histologically by measuring the density of collagen using Mallory's phosphotungstic acid hematoxylin stain solution with aniline blue. The ANOVA test was used statistically to examine variations in collagen density across groups.
Results : The histology analysis revealed that the average quantity of collagen sequentially was 67.5% for group I, 59.5% for group II, 64.9% for group III, and 71.0% for group IV. Meanwhile, statistical tests revealed that there was no significant difference between the groups, with p>0.05 significance.
Conclusion: Administrating kirinyuh leaf aqueous extract (C. odorata) did not have a significant effect on the density of collagen in mice (Mus musculus).
Keywords
References
Anggraeni, L., & Bratadiredja, M. A. (2018). Review Artikel: Tanaman Obat yang Memiliki Aktivitas Terhadap Luka Bakar. Farmaka, 16(2), 51–59.
Aro, S. O., Osho, I. B., Aletor, V. A., & Tewe, O. O. (2009). Chromolaena odorata in livestock nutrition. Journal of Medicinal Plants Research, 3(13), 1253–1257.
Cañedo-Dorantes, L., & Cañedo-Ayala, M. (2019). Skin acute wound healing: a comprehensive review. International Journal of Inflammation, 2019.
Enoch, S., & Leaper, D. J. (2008). Basic science of wound healing. Surgery (Oxford), 26(2), 31–37.
Gogoi, P., Laskar, M. A., Sen, S., Dey, B. K., Choudhury, R. A., & Borgohain, R. (2021). Ethno medicinal plants used for wound healing properties in Tinsukia district, Assam: A comprehensive review. J Pharm Res Int, 33(1), 270–292.
Guo, S. al, & DiPietro, L. A. (2010). Factors affecting wound healing. Journal of Dental Research, 89(3), 219–229.
Gwynn-Jones, D., Jones, A. G., Waterhouse, A., Winters, A., Comont, D., Scullion, J., Gardias, R., Graee, B. J., Lee, J. A., & Callaghan, T. V. (2012). Enhanced UV-B and Elevated CO2 Impacts Sub-Arctic Shrub Berry Abundance, Quality and Seed Germination. AMBIO, 41(3), 256–268.
Heinäaho, M., Pusenius, J., & Julkunen-Tiitto, R. (2006). Effects of Different Organic Farming Methods on the Concentration of Phenolic Compounds in Sea Buckthorn Leaves. Journal of Agricultural and Food Chemistry, 54(20), 7678–7685.
Isenhath, S. N., Fukano, Y., Usui, M. L., Underwood, R. A., Irvin, C. A., Marshall, A. J., Hauch, K. D., Ratner, B. D., Fleckman, P., & Olerud, J. E. (2007). A mouse model to evaluate the interface between skin and a percutaneous device. Journal of Biomedical Materials Research Part A: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials, 83(4), 915–922.
Kumar, B., Vijayakumar, M., Govindarajan, R., & Pushpangadan, P. (2007). Ethnopharmacological approaches to wound healing—Exploring medicinal plants of India. Journal of Ethnopharmacology, 114(2), 103–113.
Lavola, A., Nybakken, L., Rousi, M., Pusenius, J., Petrelius, M., Kellomäki, S., & Julkunen-Tiitto, R. (2013). Combination treatment of elevated UVB radiation, CO2 and temperature has little effect on silver birch (Betula pendula) growth and phytochemistry. Physiologia Plantarum, 149(4), 499–514.
Ngozi, I. M., Jude, I. C., & Catherine, I. C. (2009). Chemical profile of Chromolaena odorata L.(King and Robinson) leaves. Pakistan Journal of Nutrition, 8(5), 521–524.
Owoyele, V. B., Adediji, J. O., & Soladoye, A. O. (2005). Anti-inflammatory activity of aqueous leaf extract of Chromolaena odorata. Inflammopharmacology, 13(5), 479–484.
Pandith, H., Zhang, X., Liggett, J., Min, K.-W., Gritsanapan, W., & Baek, S. J. (2013). Hemostatic and wound healing properties of Chromolaena odorata leaf extract. International Scholarly Research Notices, 2013.
Rao, M. J., Ahmed, U., Ahmed, M. H., Duan, M., Wang, J., Wang, Y., & Wang, L. (2021). Comparison and quantification of metabolites and their antioxidant activities in young and mature leaves of sugarcane. ACS Food Science & Technology, 1(3), 362–373.
Sirinthipaporn, A., & Jiraungkoorskul, W. (2017). Wound healing property review of siam weed, Chromolaena odorata. Pharmacognosy Reviews, 11(21), 35.
Stanley, M. C., Ifeanyi, O. E., Nwakaego, C. C., & Esther, I. O. (2014). Antimicrobial effects of Chromolaena odorata on some human pathogens. International Journal of Current Microbiology and Applied Sciences, 3(3), 1006–1012.
Sterehi, D. L., & Keefer, L. R. (1998). Modified Mallory Aniline Blue Stain for Bone, Cartilage, and Other Connective Tissues. Journal of Histotechnology, 21(2), 129–133.
Stunova, A., & Vistejnova, L. (2018). Dermal fibroblasts—A heterogeneous population with regulatory function in wound healing. Cytokine & Growth Factor Reviews, 39, 137–150.
Suvarna, K. S., Layton, C., & Bancroft, J. D. (2018). Bancroft’s theory and practice of histological techniques E-Book. Elsevier health sciences.
Thang, P. T., Teik, L. S., & Yung, C. S. (2001). Anti-oxidant effects of the extracts from the leaves of Chromolaena odorata on human dermal fibroblasts and epidermal keratinocytes against hydrogen peroxide and hypoxanthine–xanthine oxidase induced damage. Burns, 27(4), 319–327.
Velnar, T., Bailey, T., & Smrkolj, V. (2009). The wound healing process: an overview of the cellular and molecular mechanisms. Journal of International Medical Research, 37(5), 1528–1542.
Vijayaraghavan, K., Rajkumar, J., Bukhari, S. N. A., Al Sayed, B., & Seyed, M. A. (2017). Chromolaena odorata: A neglected weed with a wide spectrum of pharmacological activities. Molecular Medicine Reports, 15(3), 1007–1016.
Vijayaraghavan, K., Rajkumar, J., & Seyed, M. A. (2017). Efficacy of Chromolaena odorata leaf extracts for the healing of rat excision wounds. Veterinární Medicína, 62(10), 565–578.
Vital, P. G., & Rivera, W. L. (2009). Antimicrobial activity and cytotoxicity of Chromolaena odorata (L. f.) King and Robinson and Uncaria perrottetii (A. Rich) Merr. Extracts. Journal of Medicinal Plants Research, 3(7), 511–518.
Yudhika, I. (2020). Pengaruh Ekstrak Jeli Daun Chromolaena odorata dalam Penyembuhan Luka Fase I dan Fase II pada Tikus Putih (Rattus novergicus). Universitas Syiah Kuala.
DOI: 10.24815/jks.v24i1.34823
Refbacks
- There are currently no refbacks.