Resistance Testing of Salmonella sp. Isolated from Broiler Chicken  against Antibiotics

Martiana Syafitri; Erina Erina; M Daud AK; Teuku Reza Ferasyi; Abdullah Hamzah; Nazaruddin Nazaruddin; Ismail Ismail

doi:10.21157/j.med.vet..v17i1.20301

Resistance Testing of Salmonella sp. Isolated from Broiler Chicken against Antibiotics

Martiana Syafitri, Erina Erina, M Daud AK, Teuku Reza Ferasyi, Abdullah Hamzah, Nazaruddin Nazaruddin, Ismail Ismail

Abstract

Antibiotic resistance has become a global problem, this happens because strains of bacteria in the body are resistant to antibiotics, one of which is caused by improper use. This study aims to determine the resistance of Salmonella sp. isolated from broiler chickens to antibiotics. The method used for this study is the Kirby Bauer disc diffusion method. The research samples were eight isolates from broiler chickens collected by the Laboratory of Microbiology, Faculty of Veterinary Medicine, Universitas Syiah Kuala. This study used antibiotics amoxicillin, streptomycin, chloramphenicol, ciprofloxacin, gentamicin, and tetracycline. The data obtained are processed descriptively. The results showed five isolates (62.5%) showed multidrug resistance and very high levels of resistance to antibiotics from eight samples tested, namely tetracycline 87.5% (7/8), chloramphenicol 75% (6/8), amoxicillin 62.5% (5/8), and streptomycin 62.5% (5/8). Antibiotics that are still sensitive are gentamicin 62.5% (5/8) and ciprofloxacin 50% (4/8). Therefore, it can be concluded that Salmonella sp. has been resistant to several types of antibiotics.

Keywords

Salmonella sp; broiler chicken; antibiotic resistance

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References

[CLSI] Clinical and Laboratory Standards Institute. 2012. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Second Informational Supplement. West Valley (US): Clinical and Laboratory Standards Institute.

Alam, S.B., Mahmud, M., Akter, R., Hasan, M., Sobur, A., Nazir, K.N.H., Noreddin, A., Rahman, T., Mohamed, E., Zowalaty, E. and Rahman, M. 2020. Molecular detection of multidrug resistant Salmonella species isolated from broiler farm in Bangladesh. Pathogens, 9(201): 2- 12.

American Meat Science Association. 2015. Salmonella Fact Sheet. Department of Animal Sciences and Food Research Institute, University of Wisconsin-Madison.

Aulia, R., Handayani, T dan Yennie, Y. 2015. Isolasi, identifikasi dan enumerasi bakteri Salmonella spp. pada hasil perikanan serta resistensinya terhadap antibiotik. BIOMA II, (1):15-33.

Carter, G.R. and Wise, D.J. 2004. Veterinary Bacteriologi and Micologi. State Press, Iowa USA. Cavalieri, S.J., Ronald, J.H., Yvette, S.M., Jose, O., Ivonne, D.R., Robert, L.S., Susan, E.S dan Carol, A.S. (2005). Manual Of Antimicrobial Susceptibility Testing. American Society for Microbiology, USA.

Chotiah, S. dan Darmayanti, R. 2014. Infeksi Salmonella enteritidis pada ayam pedaging dan pola resistensi terhadap antibiotik. Seminar Nasional Teknologi Peternakan dan Veteriner, Balai Besar Penelitian Veteriner.

Cosby, D.E., Cox, N.A., Harrison, M.A., Wilson, J.L., Buhr, R.J. and Cray, P.J.F. 2015. Salmonella and antimicrobial resistance in broilers: A review. J. Appl. Poult. Res, 1-19.

Dewi, E.S., Latifa, E.S., Fawwarahly dan Kautsar, R. 2016. Kualitas mikrobiologi daging unggas di RPA dan yang beredar di pasaran. Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan, 4(3): 379-385.

Hossain, M.A., Amin, M.R., Khan, M.D.I., Mollah, M.L. and M. A. Amin, M.A. 2015. Occurrences, treatment and antibiotic resistant pattern of colibacillosis and salmonellosis in broiler. Journal of Bioscience and Agriculture Research, 4(02):67-73.

Humaida, R. 2014. Strategy to handle resistance of antibiotik. J Majority, 3(7):113-

Hur, J., Jawale, C. and Lee, J.H. 2012. Antimikrobial resistance of Salmonella isolated from food animals: A review. Food. Res. Int, 45(2):819-830.

Indijah, S.W. dan Fajri, P. 2016. Farmakologi. Pusdik SDM Kesehatan, Jakarta.

Kholifah, L.N., Dharma, B. dan Situmeang, R. 2016. Cemaran Salmonella pada daging ayam di beberapa rumah potong ayam dan pasar tradisional Kota Samarinda dengan metode compact dry. Jurnal Kesehatan masyarakat veteriner, 1(3):383-387.

Lay, W.B. 1994. Analisis Mikroba di Laboratorium. PT Raja Grafindo Persada, Jakarta. Maka, L. and Popowska, M. (2016). Antimicrobial resistance of salmonella spp. isolated from food. Rocz Panstw Zakl Hig, 67(4):343-358.

Miol, L., Parizotto, L., Calasans, M., Dickel, E., Pilatto, F., Rodrigues, L.B., Nascimento, V.P. dan Santoso, L.R. 2016. Effect of antimicrobial on Salmonella spp. Strains isolated from poultry processing. Brazilian Journal of poultry Science,18(2):337-342.

Muharlien, Achmanu dan Rahmawati, R. 2011. Meningkatkan produksi ayam pedaging melalui pengaturan proporsi sekam, pasir dan kapur sebagai litter. Jurnal Ternak Tropika, 12(1):38- 45.

Murti, N.I.K. dan Budayanti, N.N.S. 2017. Prevalensi Salmonella sp. pada cilok di sekolah dasar di Denpasar. E-Jurnal Medika, 6(5):36-41.

Perdana, R dan Setyawati,T. 2016. Uji in-vitro sensitivitas antibiotik terhadap bakteri Salmonella Typhi di Kota Palu. Jurnal Ilmiah Kedokteran, 3(1):11-22.

Pratiwi, T., 2008. Mikrobiologi Farmasi. Erlangga, Jakarta.

Safitri, E. Hidayati, N.A. dan Hartati, R. 2019. Prevalensi bakteri Salmonella pada ayam potong yang dijual di pasar tradisional Pangkalpinang. Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi, 4(1):25-30.

Safitri, R. dan Novel, S.S. 2010. Medium Analisis Mikroorganisme (Isolat dan Kultur). Trans Info Media, Jakarta.

Siriken, B., Turk, H., Yildirim, T., Durupinar, B. And Erol, I. 2015. Prevalence and characterization of Salmonella isolated from chicken meat in Turkey. Journal of Food Science, 80(5):1044- 1050.

Soleha, T,R. 2015. Uji kepekaan terhadap antibiotik. Juke Unila, 5(9):120-123.

Timotius, K.H. 1982. Mikrobiologi Dasar. Universitas Kristen Satya Wacana Salatiga, Jawa Tengah.

Tripathi, K. D. 2003. Antimicrobial Drugs: General Consideration Essential of Medical Pharmacology. Fifth Edition. Jaypee Brothers Medical Publishers.

Umam, M.K., Prayogi, H.S. dan Nurgiartiningsih,V.M.A. 2014. Penampilan produksi ayam pedaging yang dipelihara pada sistem lantai kandang panggung dan kandang bertingkat. Jurnal Ilmu–Ilmu Peternakan, 24(3):79-87.

World Heath Organization. 2015. Antimicrobial resistance: Globa Report on Surveilance. World Healt Organization, Swizerland.

Zelpina, E., Purnawarman, T. dan Lukman, D.W. 2018. Keberadaan Salmonella sp. pada daging ayam suwir bubur ayam yang dijual di lingkar kampus institut pertanian Bogor dramaga Bogor. Jurnal Penelitian Pasca Panen Pertanian, 15(2):73-79.

DOI: https://doi.org/10.21157/j.med.vet..v17i1.20301