Histopathology of Heart Rats (Rattus norvegicus) Due to the Use of Cooking Oil

Abstract

This research aims to study the histopathological picture of rats (Rattus norvegicus) heart as a result of the provision of waste cooking oil
3x, 6x, and 9x frying. This study used 20 male white rats (Rattus norvegicus) weighing of ± 300 g. Rats were grouped into 4 treatment groups,
adapted for one week, feed and drink ad libitum. The second week to eight week rats were given orally cooking oil every day with a dose of 1 ml.
A group of rats was given cooking oil (negative control). Group B, C, and D were given cooking oil 3x, 6x, and 9x frying. Mi ce were euthanized
after 60 days, then necropsied to collect the heart. Heart was then put into Neutral Buffered Formalin (NBF) for fixation, then follow with
histopathology preparation. Parameters measured were hyperemia, hemorrhage, degeneration, necrosis of the heart. Results were analyzed
descriptively with the scoring system. Test results are grouped into three categories, namely, mild (+), moderate (++), and weight (+++). The
hystopathological results of the heart found were an increasing number of hyperemia, hemorrhage, degeneration, necrosis as a result of the
frequency of frying.
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Key words: heart, cooking oil, rats