9. Total  colony of cellulolitic bacteria in the rumen of aceh cattle

Safika Safika; Siti Wardianah Matondang; Darmawi Darmawi; Mahdi Abral; Erina Erina; M Jalaluddin

doi:10.21157/j.med.vet..v11i1.3411

9. Total colony of cellulolitic bacteria in the rumen of aceh cattle

Safika Safika, Siti Wardianah Matondang, Darmawi Darmawi, Mahdi Abral, Erina Erina, M Jalaluddin

Abstract

The aim of this study was to count the total sum of cellulolitic bacteria colony in aceh cattle rumen. The sample used was aceh cattle rumen liquid which taken from salughtered house in Banda Aceh. Aceh cattle rumen liquid were diluted from 10^-2to 10^-5, then inoculated to agar BHM-CMC media with pour plate method. Petri dish was entered to candle jar to be incubated in an incubator with temperature 37^oC about 96 hours. Colony of cellulolitic bacteria which were grown then were observed by using colony bacteria criteria (colour, position, edge, and diameter of colony), counting total of bacteria which was grown, and Gram staining. The results showed that there is a white translucent colony morphology, growth inside agar, smooth periphery, Gram-positive bacil. The average number bacteria of total bacteria in the cattle rumen liquid was 4,81 x 10⁵cfu/ml

Keywords

cellulolitic bacteria; morphology; liquid; colony; aceh cattle.

Full Text:

PDF

References

Abdullah, M.A.N., R.R. Noor, H. Martojo, D.D. Solihin, dan E. Handiwirawan. 2007. Keragaman Fenotipik Sapi Aceh di Nangroe Aceh Darussalam. J. Indon. Trop. Anim Agric. 32 (1): 11-21.

Aklyosov. 2004. Cellulase Enzyme That Degrades Celulose From Fungi. Application of Microbial. 522-529.

Ambriyanto, K. S. 2010. Isolasi dan Karakterisasi Bakteri Aerob Pendegradasi Selulosa dari Serasah Daun Rumput Gajah (Pennisetum purpureum schaum). Skripsi. Institut Teknologi Sepuluh Nopember, Surabaya.

Basri, H. 2006. Penelusuran Arah Pembibitan Sapi Aceh. Jurusan Peternakan Fakultas Pertanian Universitas Syiah Kuala Darussalam, Banda Aceh.

BPTU-HPT. 2014. Keunggulan Sapi Aceh. bptuhptindrapuri.com/keunggulan-sapi-aceh. Diakses: 3 Oktober 2015.

Cappucino, J.G., and N. Sherman. 2001. Microbiology: A Laboratory Manual, Benjamin Cummings Publishing, United State of America.

Dilly, O, S. Bartsch, P Rosenbrock, F Buscot, J.C Munch. 2001. Shifts in physiological capabilities of the microbiota during the decomposition of leaf litter in a black alder forest. Soil. Biol. Biochem. 33:921–930.

Fitria, L. dan Y. Yasmin. 2011. Isolasi dan pengamatan morfologi koloni bakteri kitinolotik. Jurnal Ilmiah Pendidikan Biologi. 3(2):20-25.

Hernawati, T., M. Lamid, H.A. Hermadi, S.H. Warsito. 2010. Bakteri seluloltik untuk meningkatkan kualitas pakan komplit berbasis limbah pertanian. Veterinaria Medika. 3(3): 43-53.

Lukman, D.W., M. Sudarwanto, A.W. Sanjaya, T. Purnawarman, H. Latif, dan Soejoedono. 2009. Higiene pangan. Laporan Penelitian. Fakultas Kedokteran Hewan Institut Pertanian Bogor, Bogor.

Lynd, L.R, P.J. Weimer, W.H.V. Zyl. I.S. and Pretorius. 2002. Microbial cellulose utilization: fundamental sand biotechnology. Microbiol Mol. Biol. 6(6): 506-577.

Marganingtyas, D.D. 2011. Potensi Selulolitik Indigenous Mangrove Terhadap Komposisi Limbah Tambak Udang. Skripsi. Fakultas Pertanian Universitas Brawijaya, Malang.

Martojo, H. 2003. Indigenous Bali Cattle: The Best Suited Cattle Breed for Sustainable Small Farms in Indonesia. Laboratory of Animal Breeding and Genetics, Faculty of Animal Science, Bogor Agricultural University, Bogor.

Meryandini, A. 2009. Isolasi bakteri selulolitik dan karakteristik enzimnya. Makara Sains 13(1):32-38

Noor, R.R. 2008. Genetika Ternak. Penebar Swadaya, Jakarta.

Pattanachomsri U, P. Kanokratana, L. Eurwilaichitr, Y. Igarashi, and Champreda V. 2011. Culture-independent phylogenetic analysis of the microbial community in industrial sugarcane bagasse feedstock piles. Biosci Biotechol Biochem 75:232–239.

Preston, T.R. and R.A. Leng. 1987. Matching Ruminant Production Sistems with Available Resources in the Tropic and Sub-Tropic. International Colour Production. Stanthorpe, Queensland, Australia.

Silva , J.R., Silva, F. J. L, And I. Sazima. 2005. Rest, nurture, sex, release, and play: diurnal underwater behaviour of the spinner dolphin at Fernando de Noronha Archipelago, SW Atlantic. Aqua Journal of Ichthyology and Aquatic Biology. 9:161-176.

Singh, S., V.S. Moholkar. and A. Goyal. 2013. Isolation, identification and characterization of a cellulolytic bacillus amyloliquefaciens strain SS35 102. J. Kaunia. 10 (2): 92-102.

Ulrich, A, G. Klimke and S. Wirth. 2008. Diversity and activity of cellulose-decomposing bacteria, isolated from a sandy and a loamy soil after long-term manure application. Microb Ecol 55: 512–522.

Waluyo, L. 2004. Mikrobiologi Umum. UMM Press, Malang.

DOI: https://doi.org/10.21157/j.med.vet..v11i1.3411