26. Histopathology view of thymus in broiler chickhen Infected by Escherichia coli And Given Kemangi Leaves (Ocimum basilicum ) Extract

Doly Ihsan Siregar; Hamdani Budiman; Dwina Aliza; Ummu Balqis; Nuzul Asmilia; Ismail Ismail

doi:10.21157/j.med.vet..v13i2.3549

26. Histopathology view of thymus in broiler chickhen Infected by Escherichia coli And Given Kemangi Leaves (Ocimum basilicum ) Extract

Doly Ihsan Siregar, Hamdani Budiman, Dwina Aliza, Ummu Balqis, Nuzul Asmilia, Ismail Ismail

Abstract

The aim of this study was to determined effect of kemangi leaves extract on level of doses to changes of microcsopic structure of broiler thymus which infected by Escherichia coli (E. coli). This study used 15 DOC that raised for 34 days. Broiler divided into 5 treatment group with 3 repetition. Negative control (A0) given standard feed, positive control (A1) given standard feed and infected with E.coli. Group A2, A3, and A4 given standard feed and infected with E. coli, added with kemangi leaves extract in concentration of 2,5%, 3,5%, and 4,5%. E. coli infected through intra peritonium as much of 0,25 ml on 15th days. Kemangi leaves extract were given from 7th days to 34th days. Observation on A1 showed many empty room. A2 and A3 showed a bit repairement on thymus microscopic structure. In A4 thymus showed approaching normal appearance as in A0. In conclusion, kemangi leaves extract capable to repair microscopic structure of broiler thymus which infected with E. coli. Kemangi leaves administration at 4,5% dose had a better effectivity rather than dose of 2,5% and 3,5%.

Keywords

Broiler; E. coli; Kemangi leaves (Ocimum basilicum); Thymus

Full Text:

PDF

References

Amrullah, I.K. 2003. Nutrisi Ayam Broiler. Lembaga Satu Gunungbudi, Bogor.

Barton, M.D. and W.S. Hart. 2001. Public health risks: Antibiotic resistance. Asian-Aus J. Animal Sci. 14:414-422.

Cushnie, T.P. dan Lamb, A.J. 2005. Antimicrobial activity of flavonoid. J. Nat. Prod. 26:343-356.

Kim, J.M., M. Marshall, R. Coenell, J.F. Bostom, and C.I. Wei. 1995. Antibacteria activity of cacrol, citral, and geraniol against Salmonella typhimurium in culture medium and on fish cubes. J. Food Sci. 43:1365-1368.

Libriani, R. 2007. Kajian Imunopatologi Sistem Limforetikular Mencit (Mus musculus) pada Persembuhan Luka Operasi dengan Pemberian Minyak Obat Luka Rantau. Skripsi. Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Bogor.

Maryati, R.S. Fauzia, dan T. Rahayu. 2007. Uji Aktivitas Antibakteri Minyak Atsiri Daun Kemangi (Ocimum basilicum) Terhadap Staphylococcus dan Escherichia coli. Jurnal Penelitian Sains dan Teknologi. 8(1):30-38.

Nurhayati, D. 2001. Imunomodulator Pada Infeksi Bakteri. Thesis. Program Studi Magister Ilmu Biomedik Universitas Diponegoro. Semarang.

Putra, R.P. 2015. Struktur Mikroskopik Hati Ayam Broiler

yang Diinfeksikan Escherichia coli dan Diberikan Ekstrak Daun Kemangi (Ocimum basilicum). Skripsi. Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh.

Sastroamodjojo. 2001. Obat Asli Indonesia Edisi ke 6. Dian Rakyat, Jakarta.

Schmidt, R.E., D.R. Reavill, and D.N. Phimen. 2003. Pathology of Pet and Aviary Birds. Blacwell Publishing. Iowa.

Siregar, C.J. 2009. Gambaran Respon Kebal Terhadap Infectious Bursal Disease (IBD) pada Ayam Pedaging yang Divaksinasi IBD Kille Setengah Dosis dan Ditantang dengan Virus IBD. Skripsi. Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Bogor.

Tabbu, C.R. 2000. Kolibasilosis dalam Penyakit Ayam dan Penanggulangannya. Kanisius. Yogyakarta.

Tarmudji. 2003. Kolibasilosis pada ayam: etiologi, patologi dan pengendaliannya. Wartazoa. 13(2). 65-73.

Wahyuni, N.Y., N. Mayasari dan Abun. 2012. Pengaruh penggunaan ekstrak kulit jengkol (Pithecellobium jiringa (Jack) Prain) dalam ransum terhadap nilai hematologi ayam broiler. Jurnal Universitas Padjajaran. 1(1): 1-5.

DOI: https://doi.org/10.21157/j.med.vet..v13i2.3549