Antidiabetic Test of Ethanolic Extract of Ant Nest Plants (Myrmecodia sp.) Against the α-Glucosidase Enzyme In Vitro

Daniel Daniel; Muhammad Isa; Muhammad Hasan; Wahyu Eka Sari; Rosmaidar Rosmaidar; Siti Aisyah; Farida Athaillah; Frengki Frengki

doi:10.21157/j.med.vet..v18i1.37431

Antidiabetic Test of Ethanolic Extract of Ant Nest Plants (Myrmecodia sp.) Against the α-Glucosidase Enzyme In Vitro

Daniel Daniel, Muhammad Isa, Muhammad Hasan, Wahyu Eka Sari, Rosmaidar Rosmaidar, Siti Aisyah, Farida Athaillah, Frengki Frengki

Abstract

The use of plants, one of the biological sources, as an alternative medicine for degenerative diseases is increasingly being studied. Usually, the biological effects of these plants come from the secondary metabolite components contained therein. Ant nest is one of them which is reported to contain compounds belonging to the class of flavonoids, tannins, and polyphenols. This research was conducted in vitro by observing the antidiabetic potential of the ethanolic extract of ant nests against the α-glucosidase enzyme following the modified "Wako Pure Chemical Industries Ltd., Japan" protocol. The results showed that the inhibitory power of α-glucosidase of the extract was stronger than that of the quercetin control with IC₅₀ values of 10.59 µg/mL and 38.09 µg/mL, respectively. These results proved that the ethanolic extract of ant nest had very strong α-glucosidase inhibitory property. Predictive analysis of the pharmacokinetics and toxicity of the bioactive compounds contained in the ant nest extract showed that rutin has similar characteristics to acarbose® as a first-line anti-diabetic drug of choice through an α-glucosidase inhibitor mechanism, so rutin is worthy of consideration for development as an anti-diabetic candidate.

Keywords

Antidiabebetes; in vitro; Myrmecodia sp; α-glucosidase

Full Text:

PDF

References

Basuki T, Indah D, Nina A, Kardono LBS. (2002). Evaluasi aktivitas daya hambat enzim α-glukosidase dari ekstrak kulit batang, daun, bunga dan buah kemuning [Murraya Paniculata (L.) Jack.]. Prosiding Seminar Nasional Tumbuhan Obat Indonesia XXI. Surabaya: Fakultas Farmasi Universitas Surabaya.

Cheng N, Yi WB, Wang QQ, et al. (2014). Synthesis and a-glucosidase inhibitory activity of chrysin, diosmetin, apigenin, and luteolin derivatives. Chin Chem Lett;25:1094–8.

Dewi RT., Iskandar YM, Hanafi M, et al., (2007). Inhibitory effect of Koji Aspergillus terreus on α-glucosidase activity and postprandial hyperglycemia. Pakistan Journal of Biological Science, 18, 3131-3135.

Engida A.M., Kasim N.S., Tsigie Y.A., Ismadji S., Huinh L.H., Ju y.H. (2013) Extraction, identification and quantitative HPLC analysis of flavonoids from sarang semut (Myrmecodia pendan). Industrial Crops and Products, 41: 392–396

Hanh M.T.T., Thao B.T.N. (2015). Study on Chemical and Biological Activities of Hydnophytum formicarum Extracts and Their Applications in Biscuits. The University Of Danang. Univ. Danang J. Sci. Technol. 91(6), 10

Hanh N.P., Phan T., Thuan N.T.D., Hanh T.T.H., Vien L.T., Thao N.P., Thanh N.P., Cuong N., Binh N.Q., Nam N.H., Kiem P.V., Kim Y.H., Minh C.V. (2016). Two new iridoid compounds from the ant-plant Myrmecodia tuberosa and their antimicrobial effects. Natural Product Research, 30: 2071-2076

Hao S W., Di-Feng Z., Chang-Xin Z., Chu-Rui F., Yi-Jia L., Bo Yanga, Qiao- Jun H.. (2009). Insulin Sensitizing Activity of Ethyl Acetate fraction of Acorus calamus L. in vitro and in vivo. Journal of Ethnopharmacology, 123, 288- 292

Hongxiang H., Tang, G., dan Liang, V.W. (2009). Hypoglycemic herbs and their_action_mechanisms._Chinese_Medicine.http://www.cmjournal.org/content/4/1/10

International Diabetes Federation. IDF Diabetes Atlas, 2015. Available from: http://www.diabetesatlas.org/

Kim, Y.M., Jeong YK., Wang MH., Lee WY., Rhee HI. (2005). Inhibitory effect of Pine extract on α-glucosidase activity and postprandial hyperglycemia. Nutrition, 21, 756-761

Koolman, J., Roehm, K.J. (2005). Color Atlas of Biochemistry (2nd ed.). New York: Thieme.

Lawson, M. A., Parrott, J. M., Mccusker, R. H., Dantzer, R., Kelley, K. W., & Connor, J. C. O. (2013). Intracerebroventricular administration of lipopolysaccharide induces indoleamine-2,3-. Journal of Neuroinflammation, 10(1), 1–9. https://doi.org/10.1186/1742-2094-10-87

Li Y., Wen S., Kota BP., Peng G., Li GQ., Yamahara J., Roufogalis BD. (2005). Punica granatum flower extract, a potent α-glucosidase inhibitor, improves postprandial hyperglycemia in Zucker diabetic fatty rats. Journal Ethnopharmacology, 99, 239-244.

Nadjib A, Hartati S, Elya B. (2011). In Vitro Bioassay of n-buthanol Isolate of Acorus calamus L. on Inhibitory of Activity α-Glucosidase. International Journal of PharmTech Research, 3(4): pp 2085-2088

Nofiyanti SH, Susilo B, Lastriyanto A. (2018). Ekstraksi Polifenol Dan Flavonoid Dari Tanaman Sarang Semut (Myrmecodia Pendans) Dengan Pretreatment Ohmic Heating. Jurnal Keteknikan Pertanian Tropis dan Biosistem; 6(3): 207-217

Olokoba AB, Obateru OA, Olokoba LB. (2012). Type 2 diabetes mellitus: a review of current trends. Oman Med J;27:269–73.

Pereira DF, Cazarolli LH, Lavado C, et al. (2011). Effects of flavonoids on a-glucosidase activity: potential targets for glucose homeostasis. Nutrition;27:1161–7.

Rasemi S, Yen K, Ahmad R, Hasan M. (2014). Total phenolic contents, antioxidant, anticancer and antidiabetic proprerties of Myrmecodia tuberosa (Rubiaceae). Council for Innovative Research, 9(3): 2000-2004

Subroto, A. dan Saputro, H. (2006). Gempur Penyakit Sarang Semut. Penerbit Swadaya, Depok

Sugiwati, S., Setiasi, S., Afifah, E. (2009). Antihyperglycemic activity of the mahkota dewa [Phaleria macrocarpa (Scheff.) boerl.] leaf extracts as an alpha-glucosidase inhibitor. Makara, Kesehatan, 13, 2, 74-78.

Taylor SI, Accili D, Imai Y. (1994). Insulin resistance or insulin deficiency. Which is the primary cause of NIDDM? Diabetes;43:735–40.

Wilke T, Boettger B, Berg B, et al. (2015) Epidemiology of urinary tract infections in type 2 diabetes mellitus patients: an analysis based on a large sample of 456,586 German T2DM patients. J Diabetes Complicat; 29:1015–23.

DOI: https://doi.org/10.21157/j.med.vet..v18i1.37431